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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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2014-April Volume 7 Issue 4

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Effect of CXCL12/CXCR4 on increasing the metastatic potential of non‑small cell lung cancer in vitro is inhibited through the downregulation of CXCR4 chemokine receptor expression

  • Authors:
    • Songping Xie
    • Wenhui Zeng
    • Guohua Fan
    • Jie Huang
    • Ganjun Kang
    • Qing Geng
    • Bangchang Cheng
    • Wei Wang
    • Ping Dong
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 941-947
    |
    Published online on: January 29, 2014
       https://doi.org/10.3892/ol.2014.1837
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Abstract

Lung cancer ranks as the most common type of cancer in males worldwide. Although great advances have been achieved in chemotherapy and radiotherapy, the long‑term survival rate of lung cancer patients has not improved significantly. Dissemination of lung cancer in the thoracic cavity and metastatic spread to the liver, bone and brain are characteristic of non‑small cell lung cancer (NSCLC), constituting the primary source of morbidity and mortality in lung cancer. Increasing evidence also indicates that the CXC chemokine receptor 4 (CXCR4)/chemokine CXC motif ligand 12 (CXCL12) chemokine axis is important for the cell invasion and migration of lung cancer. CXCR4 is a G protein‑coupled receptor with a major role in lymphocyte homing. Its ligand, CXCL12, is secreted by target organs and functions as a highly efficient chemotactic factor for T cells, monocytes, pre‑B cells, dendritic cells and myeloid bone marrow‑derived cells. In the current study, recombinant CXCR4‑specific small interfering RNA‑pBSilence1.1 plasmids were constructed and transfected into the A549 NSCLC cell line in vitro. Reverse transcription polymerase chain reaction and western blotting revealed that CXCR4 was downregulated in transfected cells compared with control cells. The results of MTT and Transwell migration assays indicated that the specific downregulation of CXCR4 inhibited cell growth, invasiveness and migration. Thus, siRNA targeting of CXCR4 may effectively inhibit the effect of CXCL12/CXCR4 on increasing the metastatic potential of NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Xie S, Zeng W, Fan G, Huang J, Kang G, Geng Q, Cheng B, Wang W and Dong P: Effect of CXCL12/CXCR4 on increasing the metastatic potential of non‑small cell lung cancer in vitro is inhibited through the downregulation of CXCR4 chemokine receptor expression. Oncol Lett 7: 941-947, 2014.
APA
Xie, S., Zeng, W., Fan, G., Huang, J., Kang, G., Geng, Q. ... Dong, P. (2014). Effect of CXCL12/CXCR4 on increasing the metastatic potential of non‑small cell lung cancer in vitro is inhibited through the downregulation of CXCR4 chemokine receptor expression. Oncology Letters, 7, 941-947. https://doi.org/10.3892/ol.2014.1837
MLA
Xie, S., Zeng, W., Fan, G., Huang, J., Kang, G., Geng, Q., Cheng, B., Wang, W., Dong, P."Effect of CXCL12/CXCR4 on increasing the metastatic potential of non‑small cell lung cancer in vitro is inhibited through the downregulation of CXCR4 chemokine receptor expression". Oncology Letters 7.4 (2014): 941-947.
Chicago
Xie, S., Zeng, W., Fan, G., Huang, J., Kang, G., Geng, Q., Cheng, B., Wang, W., Dong, P."Effect of CXCL12/CXCR4 on increasing the metastatic potential of non‑small cell lung cancer in vitro is inhibited through the downregulation of CXCR4 chemokine receptor expression". Oncology Letters 7, no. 4 (2014): 941-947. https://doi.org/10.3892/ol.2014.1837
Copy and paste a formatted citation
x
Spandidos Publications style
Xie S, Zeng W, Fan G, Huang J, Kang G, Geng Q, Cheng B, Wang W and Dong P: Effect of CXCL12/CXCR4 on increasing the metastatic potential of non‑small cell lung cancer in vitro is inhibited through the downregulation of CXCR4 chemokine receptor expression. Oncol Lett 7: 941-947, 2014.
APA
Xie, S., Zeng, W., Fan, G., Huang, J., Kang, G., Geng, Q. ... Dong, P. (2014). Effect of CXCL12/CXCR4 on increasing the metastatic potential of non‑small cell lung cancer in vitro is inhibited through the downregulation of CXCR4 chemokine receptor expression. Oncology Letters, 7, 941-947. https://doi.org/10.3892/ol.2014.1837
MLA
Xie, S., Zeng, W., Fan, G., Huang, J., Kang, G., Geng, Q., Cheng, B., Wang, W., Dong, P."Effect of CXCL12/CXCR4 on increasing the metastatic potential of non‑small cell lung cancer in vitro is inhibited through the downregulation of CXCR4 chemokine receptor expression". Oncology Letters 7.4 (2014): 941-947.
Chicago
Xie, S., Zeng, W., Fan, G., Huang, J., Kang, G., Geng, Q., Cheng, B., Wang, W., Dong, P."Effect of CXCL12/CXCR4 on increasing the metastatic potential of non‑small cell lung cancer in vitro is inhibited through the downregulation of CXCR4 chemokine receptor expression". Oncology Letters 7, no. 4 (2014): 941-947. https://doi.org/10.3892/ol.2014.1837
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