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Article

microRNA‑503 inhibits gastric cancer cell growth and epithelial‑to‑mesenchymal transition

  • Authors:
    • Yang Peng
    • Yan‑Min Liu
    • Lu‑Chun Li
    • Lu‑Lu Wang
    • Xiao‑Ling Wu
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China
  • Pages: 1233-1238
    |
    Published online on: February 11, 2014
       https://doi.org/10.3892/ol.2014.1868
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Abstract

Epithelial‑to‑mesenchymal transition (EMT) is believed to be associated with cancer cell malignancy, and also to cause cancer invasion and metastasis. Recent evidence indicates that small non‑protein coding RNA [microRNAs (miRNAs/miRs)] may act as powerful regulators of EMT. The present study aimed to systematically delineate miR‑503 expression in gastric cancer and analyse the function of miR‑503 in gastric cancer EMT. In the present study, miR‑503 expression was detected in gastric cancer cell lines and gastric cancer tissues by quantitative polymerase chain reaction. Gastric cancer cell migration, invasion and proliferation capabilities were analysed by Transwell, MTT and clonability assays. The expression of mesenchymal markers, including fibronectin, vimentin, N‑cadherin, SNAIL and the epithelial marker, E‑cadherin, was examined by immunoblot analysis following miR‑503 transfection. miR‑503 expression was found to be reduced in gastric cancer cell lines compared with normal gastric mucosa cell lines, and the expression of miR‑503 was upregulated in non‑metastatic‑derived gastric cancer cell lines compared with metastatic‑derived lines. miR‑503 expression levels were significantly reduced in tumour tissues in comparison with adjacent normal mucosa tissues, and the miR‑503 expression levels in patients with metastases were significantly lower than those in patients without. miR‑503 inhibited gastric cancer cell migration, invasion and proliferation. Fibronectin, vimentin, N‑cadherin and SNAIL protein levels were decreased, but E‑cadherin expression was increased in an AGS cell line transfected with miR‑503. Taken together, the present findings indicate that miR‑503 acts as a novel tumour suppressor gene in gastric cancer and can inhibit EMT in gastric cancer cells.
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1 

Rana TM: Illuminating the silence: understanding the structure and function of small RNAs. Nat Rev Mol Cell Biol. 8:23–36. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Valencia-Sanchez MA, Liu J, Hannon GJ and Parker R: Control of translation and mRNA degradation by miRNAs and siRNAs. Genes Dev. 20:515–524. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Pillai RS, Bhattacharyya SN and Filipowicz W: Repression of protein synthesis by miRNAs: how many mechanisms? Trends Cell Biol. 17:118–126. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Lu J, Getz G, Miska EA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Thomson JM, Newman M, Parker JS, Morin-Kensicki EM, Wright T and Hammond SM: Extensive post-transcriptional regulation of microRNAs and its implications for cancer. Genes Dev. 20:2202–2207. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Kalluri R and Weinberg RA: The basics of epithelial-mesenchymal transition. J Clin Invest. 119:1420–1428. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Thiery JP, Acloque H, Huang RY, et al: Epithelial-mesenchymal transitions in development and disease. Cell. 139:871–890. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Gibbons DL, Lin W, Creighton CJ, et al: Contextual extracellular cues promote tumor cell EMT and metastasis by regulating miR-200 family expression. Genes Dev. 23:2140–2151. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Zhao X, Dou W, He L, et al: MicroRNA-7 functions as an anti-metastatic microRNA in gastric cancer by targeting insulin-like growth factor-1 receptor. Oncogene. 32:1363–1372. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Kong W, Yang H, He L, et al: MicroRNA-155 is regulated by the transforming growth factor beta/Smad pathway and contributes to epithelial cell plasticity by targeting RhoA. Mol Cell Biol. 28:6773–6784. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Korpal M, Lee ES, Hu G and Kang Y: The miR-200 family inhibits epithelial-mesenchymal transition and cancer cell migration by direct targeting of E-cadherin transcriptional repressors ZEB1 and ZEB2. J Biol Chem. 283:14910–14914. 2008. View Article : Google Scholar

12 

Gebeshuber CA, Zatloukal K and Martinez J: miR-29a suppresses tristetraprolin, which is a regulator of epithelial polarity and metastasis. EMBO Rep. 10:400–405. 2009. View Article : Google Scholar

13 

Cottonham CL, Kaneko S and Xu L: miR-21 and miR-31 converge on TIAM1 to regulate migration and invasion of colon carcinoma cells. J Biol Chem. 285:35293–35302. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Eades G, Yao Y, Yang M, Zhang Y, Chumsri S and Zhou Q: miR-200a regulates SIRT1 expression and epithelial to mesenchymal transition (EMT)-like transformation in mammary epithelial cells. J Biol Chem. 286:25992–26002. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Gregory PA, Bert AG, Paterson EL, et al: The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1. Nat Cell Biol. 10:593–601. 2008. View Article : Google Scholar

16 

Du R, Sun W, Xia L, et al: Hypoxia-induced down-regulation of microRNA-34a promotes EMT by targeting the Notch signaling pathway in tubular epithelial cells. PLoS One. 7:e307712012. View Article : Google Scholar

17 

Xu YY, Wu HJ, Ma HD, Xu LP, Huo Y and Yin LR: MicroRNA-503 suppresses proliferation and cell cycle progression of endometrioid endometrial cancer via negatively regulating cyclin D1. FEBS J. 280:3768–3779. 2013. View Article : Google Scholar

18 

Luo HC, Zhang ZZ, Zhang X, Ning B, Guo JJ, Nie N, Liu B and Wu XL: microRNA expression signature in gastric cancer. Chin J Cancer Res. 21:74–80. 2009. View Article : Google Scholar

19 

Zhang Y, Chen X, Lian H, et al: MicroRNA-503 acts as a tumor suppressor in glioblastoma for multiple antitumor effects by targeting IGF-1R. Oncol Rep. 31:1445–1452. 2014.PubMed/NCBI

20 

Zhao JJ, Yang J, Lin J, et al: Identification of miRNAs associated with tumorigenesis of retinoblastoma by miRNA microarray analysis. Childs Nerv Syst. 25:13–20. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Corbetta S, Vaira V, Guarnieri V, et al: Differential expression of microRNAs in human parathyroid carcinomas compared with normal parathyroid tissue. Endocr Relat Cancer. 17:135–146. 2010. View Article : Google Scholar

22 

Özata DM, Caramuta S, Velázquez-Fernández D, et al: The role of microRNA deregulation in the pathogenesis of adrenocortical carcinoma. Endocr Relat Cancer. 18:643–655. 2011.PubMed/NCBI

23 

Lu YC, Chen YJ, Wang HM, et al: Oncogenic function and early detection potential of miRNA-10b in oral cancer as identified by microRNA profiling. Cancer Prev Res (Phila). 5:665–674. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Watahiki A, Wang Y, Morris J, et al: MicroRNAs associated with metastatic prostate cancer. PLoS One. 6:e249502011. View Article : Google Scholar : PubMed/NCBI

25 

Jiang Q, Feng MG and Mo YY: Systematic validation of predicted microRNAs for cyclin D1. BMC Cancer. 9:1942009. View Article : Google Scholar : PubMed/NCBI

26 

Caporali A, Meloni M, Völlenkle C, et al: Deregulation of microRNA-503 contributes to diabetes mellitus-induced impairment of endothelial function and reparative angiogenesis after limb ischemia. Circulation. 123:282–291. 2011. View Article : Google Scholar

27 

Zhou B, Ma R, Si W, et al: MicroRNA-503 targets FGF2 and VEGFA and inhibits tumor angiogenesis and growth. Cancer Lett. 333:159–169. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Zhou J and Wang W: Analysis of microRNA expression profiling identifies microRNA-503 regulates metastatic function in hepatocellular cancer cell. J Surg Oncol. 104:278–283. 2011. View Article : Google Scholar : PubMed/NCBI

29 

Kurashige J, Kamohara H, Watanabe M, et al: MicroRNA-200b regulates cell proliferation, invasion, and migration by directly targeting ZEB2 in gastric carcinoma. Ann Surg Oncol. 19(Suppl 3): S656–S664. 2012. View Article : Google Scholar

30 

Zhang Z, Liu S, Shi R and Zhao G: miR-27 promotes human gastric cancer cell metastasis by inducing epithelial-to-mesenchymal transition. Cancer Genet. 204:486–491. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Peng Y, Liu YM, Li LC, Wang LL and Wu XL: microRNA‑503 inhibits gastric cancer cell growth and epithelial‑to‑mesenchymal transition. Oncol Lett 7: 1233-1238, 2014.
APA
Peng, Y., Liu, Y., Li, L., Wang, L., & Wu, X. (2014). microRNA‑503 inhibits gastric cancer cell growth and epithelial‑to‑mesenchymal transition. Oncology Letters, 7, 1233-1238. https://doi.org/10.3892/ol.2014.1868
MLA
Peng, Y., Liu, Y., Li, L., Wang, L., Wu, X."microRNA‑503 inhibits gastric cancer cell growth and epithelial‑to‑mesenchymal transition". Oncology Letters 7.4 (2014): 1233-1238.
Chicago
Peng, Y., Liu, Y., Li, L., Wang, L., Wu, X."microRNA‑503 inhibits gastric cancer cell growth and epithelial‑to‑mesenchymal transition". Oncology Letters 7, no. 4 (2014): 1233-1238. https://doi.org/10.3892/ol.2014.1868
Copy and paste a formatted citation
x
Spandidos Publications style
Peng Y, Liu YM, Li LC, Wang LL and Wu XL: microRNA‑503 inhibits gastric cancer cell growth and epithelial‑to‑mesenchymal transition. Oncol Lett 7: 1233-1238, 2014.
APA
Peng, Y., Liu, Y., Li, L., Wang, L., & Wu, X. (2014). microRNA‑503 inhibits gastric cancer cell growth and epithelial‑to‑mesenchymal transition. Oncology Letters, 7, 1233-1238. https://doi.org/10.3892/ol.2014.1868
MLA
Peng, Y., Liu, Y., Li, L., Wang, L., Wu, X."microRNA‑503 inhibits gastric cancer cell growth and epithelial‑to‑mesenchymal transition". Oncology Letters 7.4 (2014): 1233-1238.
Chicago
Peng, Y., Liu, Y., Li, L., Wang, L., Wu, X."microRNA‑503 inhibits gastric cancer cell growth and epithelial‑to‑mesenchymal transition". Oncology Letters 7, no. 4 (2014): 1233-1238. https://doi.org/10.3892/ol.2014.1868
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