Expression of RECK and MMP‑2 in salivary adenoid cystic carcinoma: Correlation with tumor progression and patient prognosis

Zhou,Xiaoqing; Huang,Shengyun; Jiang,Licheng; Zhang,Shizhou; Li,Wengang; Chen,Zhanwei; Zhang,Dongsheng

doi:10.3892/ol.2014.1906

Expression of RECK and MMP‑2 in salivary adenoid cystic carcinoma: Correlation with tumor progression and patient prognosis

Authors:
- Xiaoqing Zhou
- Shengyun Huang
- Licheng Jiang
- Shizhou Zhang
- Wengang Li
- Zhanwei Chen
- Dongsheng Zhang
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Affiliations: Department of Oral and Maxillofacial Surgery, First People's Hospital of Jining, Jining, Shandong 272111, P.R. China, Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Oral and Maxillofacial Surgery, Liaocheng Hospital, Liaocheng, Shandong 252000, P.R. China
Published online on: February 21, 2014 https://doi.org/10.3892/ol.2014.1906
Pages: 1549-1555

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Abstract

Reversion‑inducing cysteine‑rich protein with Kazal motifs (RECK), a glycosylphosphatidylinositol‑anchored glycoprotein, inhibits the enzymatic activities of certain matrix metalloproteinases (MMPs). RECK has been studied in numerous human tumors, but the expression of RECK in salivary adenoid cystic carcinoma (SACC), and its correlation with patient prognosis, has never been investigated thus far. In the present study, the expression of RECK and MMP‑2 was evaluated in two ACC cell lines and in 83 patients with SACC. The results of quantitative polymerase chain reaction and western blot analysis revealed that the ACC‑2 and ACC‑M cell lines expressed RECK and MMP‑2 mRNA and protein. The immunohistochemical staining in the patients demonstrated that positive expression of RECK and MMP‑2 was observed in 21/83 (25.3%) and 69/83 (83.1%) cases, respectively, and that RECK expression was significantly associated with the tumor‑node‑metastasis stage, histological grade and perineural invasion of patients with SACC (P<0.05). Furthermore, there was a significant association between the positive expression of RECK and that of MMP‑2 (P<0.0001). Univariate and multivariate analyses confirmed that a lack of RECK expression was an independent and significant factor for the prediction of a poor prognosis. In conclusion, RECK is a promising prognostic marker and potential therapeutic agent in SACC.

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