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Article

786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro

  • Authors:
    • Gang Chen
    • Yao Zhang
    • Xiaohou Wu
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China
  • Pages: 1576-1580
    |
    Published online on: March 11, 2014
       https://doi.org/10.3892/ol.2014.1962
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Abstract

Emerging evidence indicates that cancer‑derived exosomes contribute to angiogenesis, tumor immunology and invasion. However, whether cancer cell‑derived exosomes regulate the migration and invasion of the cancer cell itself, and the underlying mechanisms are not well understood. In the present study, exosomes derived from the 786‑0 human renal cancer cell line were isolated, purified and 100 µg̸ml were co‑cultured with 786‑0 cells for 24 h. The 786‑0 cells were harvested for a cell invasion and migration assay. The expression of chemokine receptor type 4 (CXCR4) and matrix metalloproteinase‑9 (MMP‑9) in the 786‑0 cells was examined by western blot analysis and revealed that the migration and invasion capabilities of the 786‑0 cells were increased, however, the cell adhesion abilities were decreased as a result of the 24‑h treatment with 786‑0‑derived exosomes. Furthermore, the expression levels of CXCR4 and MMP‑9 in the 786‑0 cells were increased. In conclusion, the 786‑0 renal cancer cell line‑derived exosomes increased migration and invasion, however, they decreased the adhesion ability of the 786‑0 cells. The exosomes may have increased the CXCR4 and MMP‑9 expression levels in the 786‑0 cells. These findings indicated that renal tumor‑derived exosomes may contribute to renal cancer development and progression.
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1 

Ferlay J, Parkin DM and Steliarova-Foucher E: Estimates of cancer incidence and mortality in Europe in 2008. Eur J Cancer. 46:765–781. 2010. View Article : Google Scholar : PubMed/NCBI

2 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics. CA Cancer J Clin. 60:277–300. 2010.

3 

Milella M and Felici A: Biology of metastatic renal cell carcinoma. J Cancer. 2:369–373. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Théry C, Ostrowski M and Segura E: Membrane vesicles as conveyors of immune responses. Nat Rev Immunol. 9:581–593. 2009.PubMed/NCBI

5 

Balaj L, Lessard R, Dai L, Cho YJ, Pomeroy SL, Breakefield XO and Skog J: Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences. Nat Commun. 2:1802011. View Article : Google Scholar : PubMed/NCBI

6 

Valadi H, Ekström K, Bossios A, Sjöstrand M, Lee JJ and Lötvall JO: Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 9:654–659. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Kahlert C and Kalluri R: Exosomes in tumor microenvironment influence cancer progression and metastasis. J Mol Med (Berl). 91:431–437. 2013. View Article : Google Scholar

8 

Yang L, Wu XH, Wang D, Luo CL and Chen LX: Bladder cancer cell-derived exosomes inhibit tumor cell apoptosis and induce cell proliferation in vitro. Mol Med Rep. 8:1272–1278. 2013.PubMed/NCBI

9 

Zhang Y, Luo CL, He BC, Zhang JM, Cheng G and Wu XH: Exosomes derived from IL-12-anchored renal cancer cells increase induction of specific antitumor response in vitro: A novel vaccine for renal cell carcinoma. Int J Oncol. 36:133–140. 2010.

10 

Weiss C, Schulze B, Ottinger A and Rödel C: To combine or not combine: the role of radiotherapy and targeted agents in the treatment for renal cell carcinoma. World J Urol. May 8–2013.(Epub ahead of print).

11 

Coppin C, Kollmannsberger C, Le L, Porzsolt F and Wilt TJ: Targeted therapy for advanced renal cell cancer (RCC): a Cochrane systematic review of published randomised trials. BJU Int. 108:1556–1563. 2011. View Article : Google Scholar

12 

Yang C and Robbins PD: The roles of tumor-derived exosomes in cancer pathogenesis. Clin Dev Immunol. 2011:8428492011. View Article : Google Scholar : PubMed/NCBI

13 

Samara GJ, Lawrence DM, Chiarelli CJ, Valentino MD, Lyubsky S, Zucker S and Vaday GG: CXCR4-mediated adhesion and MMP-9 secretion in head and neck squamous cell carcinoma. Cancer Lett. 214:231–241. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Yu T, Wu Y, Helman JI, Wen Y, Wang C and Li L: CXCR4 promotes oral squamous cell carcinoma migration and invasion through inducing expression of MMP-9 and MMP-13 via the ERK signaling pathway. Mol Cancer Res. 9:161–172. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Park B, Sung B, Yadav VR, Cho SG, Liu M and Aggarwal BB: Acetyl-11-keto-β-boswellic acid suppresses invasion of pancreatic cancer cells through the downregulation of CXCR4 chemokine receptor expression. Int J Cancer. 129:23–33. 2011.

16 

Raman D, Baugher PJ, Thu YM and Richmond A: Role of chemokines in tumor growth. Cancer Lett. 256:137–165. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Orimo A, Gupta PB, Sgroi DC, Arenzana-Seisdedos F, Delaunay T, Naeem R, Carey VJ, Richardson AL and Weinberg RA: Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Cell. 121:335–348. 2005. View Article : Google Scholar

18 

Manu KA, Shanmugam MK, Rajendran P, Li F, Ramachandran L, Hay HS, Kannaiyan R, Swamy SN, Vali S, Kapoor S, et al: Plumbagin inhibits invasion and migration of breast and gastric cancer cells by downregulating the expression of chemokine receptor CXCR4. Mol Cancer. 10:1072011. View Article : Google Scholar : PubMed/NCBI

19 

Wang L, Chen W, Gao L, Yang Q, Liu B, Wu Z, Wang Y and Sun Y: High expression of CXCR4, CXCR7 and SDF-1 predicts poor survival in renal cell carcinoma. World J Surg Oncol. 10:2122012. View Article : Google Scholar : PubMed/NCBI

20 

Gahan JC, Gosalbez M, Yates T, Young EE, Escudero DO, Chi A, Garcia-Roig M, Satyanarayana R, Soloway MS, Bird VG and Lokeshwar VB: Chemokine and chemokine receptor expression in kidney tumors: molecular profiling of histological subtypes and association with metastasis. J Urol. 187:827–833. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Zhao M, Gao Y, Wang L, Liu S, Han B, Ma L, Ling Y, Mao S and Wang X: Overexpression of integrin-linked kinase promotes lung cancer cell migration and invasion via NF-κB-mediated upregulation of matrix metalloproteinase-9. Int J Med Sci. 10:995–1002. 2013.

22 

Jia W, Gao XJ, Zhang ZD, Yang ZX and Zhang G: S100A4 silencing suppresses proliferation, angiogenesis and invasion of thyroid cancer cells through downregulation of MMP-9 and VEGF. Eur Rev Med Pharmacol Sci. 17:1495–1508. 2013.

23 

Shiau MY, Fan LC, Yang SC, Tsao CH, Lee H, Cheng YW, Lai LC and Chang YH: Human papillomavirus up-regulates MMP-2 and MMP-9 expression and activity by inducing interleukin-8 in lung adenocarcinomas. PLoS One. 8:e544232013. View Article : Google Scholar : PubMed/NCBI

24 

Dai ZJ, Wang BF, Lu WF, Wang ZD, Ma XB, Min WL, Kang HF, Wang XJ and Wu WY: Total flavonoids of Scutellaria barbata inhibit invasion of hepatocarcinoma via MMP/TIMP in vitro. Molecules. 18:934–950. 2013.

25 

Wang X, Lee SO, Xia S, Jiang Q, Luo J, Li L, Yeh S and Chang C: Endothelial cells enhance prostate cancer metastasis via IL-6→androgen receptor →TGF-β →MMP-9 signals. Mol Cancer Ther. 12:1026–1037. 2013.PubMed/NCBI

26 

Leifler KS, Svensson S, Abrahamsson A, Bendrik C, Robertson J, Gauldie J, Olsson AK and Dabrosin C: Inflammation induced by MMP-9 enhances tumor regression of experimental breast cancer. J Immunol. 190:4420–4430. 2013. View Article : Google Scholar : PubMed/NCBI

27 

DI Carlo A: Evaluation of neutrophil gelatinase-associated lipocalin (NGAL), matrix metalloproteinase-9 (MMP-9) and their complex MMP-9/NGAL in sera and urine of patients with kidney tumors. Oncol Lett. 5:1677–1681. 2013.

28 

Xue YJ, Xiao RH, Long DZ, Zou XF, Wang XN, Zhang GX, Yuan YH, Wu GQ, Yang J, Wu YT, et al: Overexpression of FoxM1 is associated with tumor progression in patients with clear cell renal cell carcinoma. J Transl Med. 10:2002012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Chen G, Zhang Y and Wu X: 786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro. Oncol Lett 7: 1576-1580, 2014.
APA
Chen, G., Zhang, Y., & Wu, X. (2014). 786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro. Oncology Letters, 7, 1576-1580. https://doi.org/10.3892/ol.2014.1962
MLA
Chen, G., Zhang, Y., Wu, X."786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro". Oncology Letters 7.5 (2014): 1576-1580.
Chicago
Chen, G., Zhang, Y., Wu, X."786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro". Oncology Letters 7, no. 5 (2014): 1576-1580. https://doi.org/10.3892/ol.2014.1962
Copy and paste a formatted citation
x
Spandidos Publications style
Chen G, Zhang Y and Wu X: 786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro. Oncol Lett 7: 1576-1580, 2014.
APA
Chen, G., Zhang, Y., & Wu, X. (2014). 786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro. Oncology Letters, 7, 1576-1580. https://doi.org/10.3892/ol.2014.1962
MLA
Chen, G., Zhang, Y., Wu, X."786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro". Oncology Letters 7.5 (2014): 1576-1580.
Chicago
Chen, G., Zhang, Y., Wu, X."786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro". Oncology Letters 7, no. 5 (2014): 1576-1580. https://doi.org/10.3892/ol.2014.1962
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