786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro

Chen,Gang; Zhang,Yao; Wu,Xiaohou

doi:10.3892/ol.2014.1962

786-0 Renal cancer cell line-derived exosomes promote 786-0 cell migration and invasion in vitro

Authors:
- Gang Chen
- Yao Zhang
- Xiaohou Wu
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Affiliations: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Yuzhong, Chongqing 400016, P.R. China
Published online on: March 11, 2014 https://doi.org/10.3892/ol.2014.1962
Pages: 1576-1580

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Abstract

Emerging evidence indicates that cancer‑derived exosomes contribute to angiogenesis, tumor immunology and invasion. However, whether cancer cell‑derived exosomes regulate the migration and invasion of the cancer cell itself, and the underlying mechanisms are not well understood. In the present study, exosomes derived from the 786‑0 human renal cancer cell line were isolated, purified and 100 µg̸ml were co‑cultured with 786‑0 cells for 24 h. The 786‑0 cells were harvested for a cell invasion and migration assay. The expression of chemokine receptor type 4 (CXCR4) and matrix metalloproteinase‑9 (MMP‑9) in the 786‑0 cells was examined by western blot analysis and revealed that the migration and invasion capabilities of the 786‑0 cells were increased, however, the cell adhesion abilities were decreased as a result of the 24‑h treatment with 786‑0‑derived exosomes. Furthermore, the expression levels of CXCR4 and MMP‑9 in the 786‑0 cells were increased. In conclusion, the 786‑0 renal cancer cell line‑derived exosomes increased migration and invasion, however, they decreased the adhesion ability of the 786‑0 cells. The exosomes may have increased the CXCR4 and MMP‑9 expression levels in the 786‑0 cells. These findings indicated that renal tumor‑derived exosomes may contribute to renal cancer development and progression.

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