Characterization of sonic hedgehog inhibition in gastric carcinoma cells

Bai,Ruxue; Zhao,Hongchuan; Zhang,Xiang; Du,Shiyu

doi:10.3892/ol.2014.1964

Characterization of sonic hedgehog inhibition in gastric carcinoma cells

Authors:
- Ruxue Bai
- Hongchuan Zhao
- Xiang Zhang
- Shiyu Du
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Affiliations: Department of Gastroenterology, China‑Japan Friendship Hospital, Chaoyang, Beijing 100029, P.R. China, Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, USA
Published online on: March 11, 2014 https://doi.org/10.3892/ol.2014.1964
Pages: 1381-1384

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Abstract

Aberrant activation of the sonic hedgehog (Shh) signaling pathway plays an important role in gastric cancer. The exact mechanisms defining how the Shh pathway promotes tumorigenesis or regulates its downstream targets remains elusive. In the present study, the effects of inhibiting the Shh signaling pathway in gastric cancer AGS cells was examined. It was identified that the Shh antagonist, cyclopamine, inhibited cancer proliferation, migration and invasion in a dose‑ and time‑dependent manner. Additionally, it was revealed that several key targets that are activated by the Shh signaling pathway, Gli1 and CXCR4, were downregulated at an RNA and protein level by cyclopamine. The results from the present study may be of benefit in facilitating the development of novel therapeutic strategies to treat gastric cancer in human patients.

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