Mixed lineage leukemia‑septin 5 fusion transcript in de novo adult acute myeloid leukemia with t(11;22)(q23;q11.2): A case report

Gao,Wen; Wang,Tong; Wu,Yin; Liu,Hong  Xing; Li,Yan  Chen; Chen,Wen  Ming

doi:10.3892/ol.2014.1971

June-2014 Volume 7 Issue 6

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June-2014 Volume 7 Issue 6

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Case Report

Mixed lineage leukemia‑septin 5 fusion transcript in de novo adult acute myeloid leukemia with t(11;22)(q23;q11.2): A case report

Authors:
- Wen Gao
- Tong Wang
- Yin Wu
- Hong Xing Liu
- Yan Chen Li
- Wen Ming Chen
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Affiliations: Department of Hematology, Myeloma Research Center of Beijing, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China, Center of Lu Dao‑Pei Hematology·Neoplasm, He‑Bei Yan Da Hospital, Langfang, Hebei 065201, P.R. China, Center of Lu Dao‑Pei Hematology·Neoplasm, He‑Bei Yan Da Hospital, Langfang, Hebei 065201, P.R. China
Pages: 1930-1932
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Published online on: March 14, 2014

https://doi.org/10.3892/ol.2014.1971
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Abstract

The current report presents a case of de novo acute myeloid leukemia (AML) in a 32‑year‑old male. Cytogenetic analysis showed that the karyotype of the bone marrow cells was as follows: 46,XY,t(11;22)(q23;q11.2)[13]/46,X,‑Y,+10,t(11;22)(q23;q11.2)[7]/47,XY,+10,t(11;22)(q23;q11.2)[1]/46,XY[1]. Fluorescence in situ hybridization analysis using a mixed lineage leukemia (MLL)‑specific probe showed a split in the MLL gene. Reverse transcription polymerase chain reaction (PCR) analysis demonstrated an MLL‑septin 5 (SEPT5) fusion transcript in the patient. Nucleotide sequencing analysis of the PCR product confirmed the fusion between the MLL exon 9 and SEPT5 exon 3, and the product was 521 bp in length. The present study reviewed the clinical and molecular features of the AML with an MLL‑SEPT5 fusion gene.

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1	Pui CH, Kane JR and Crist WM: Biology and treatment of infant leukemias. Leukemia. 9:762–769. 1995.PubMed/NCBI
2	Balgobind BV, Raimondi SC, Harbott J, et al: Novel prognostic subgroups in childhood 11q23/MLL-rearranged acute myeloid leukemia: results of an international retrospective study. Blood. 114:2489–2496. 2009. View Article : Google Scholar
3	Meyer C, Schneider B, Jakob S, et al: The MLL recombinome of acute leukemias. Leukemia. 20:777–784. 2006. View Article : Google Scholar
4	Meyer C, Kowarz E, Hofmann J, et al: New insights to the MLL recombinome of acute leukemias. Leukemia. 23:1490–1499. 2009. View Article : Google Scholar : PubMed/NCBI
5	Cerveira N, Bizarro S and Teixeira MR: MLL-SEPTIN gene fusions in hematological malignancies. Biol Chem. 392:713–724. 2011. View Article : Google Scholar : PubMed/NCBI
6	Launay E, Henry C, Meyer C, et al: MLL-SEPT5 fusion transcript in infant acute myeloid leukemia with t(11;22)(q23;q11). Leuk Lymphoma. Jun 3–2013.(Epub ahead of print).
7	Abdou SM, Jadayel DM, Min T, et al: Incidence of MLL rearrangement in acute myeloid leukemia, and a CALM-AF10 fusion in M4 type acute myeloblastic leukemia. Leuk Lymphoma. 43:89–95. 2002. View Article : Google Scholar : PubMed/NCBI
8	Tatsumi K, Taki T, Taniwaki M, et al: The CDCREL1 gene fused to MLL in de novo acute myeloid leukemia with t(11;22)(q23;q11.2) and its frequent expression in myeloid leukemia cell lines. Genes Chromosomes Cancer. 30:230–235. 2001.
9	Megonigal MD, Rappaport EF, Jones DH, et al: t(11;22)(q23;q11.2) in acute myeloid leukemia of infant twins fuses MLL with hCDCrel, a cell division cycle gene in the genomic region of deletion in DiGeorge and velocardiofacial syndromes. Proc Natl Acad Sci USA. 11:6413–6418. 1998. View Article : Google Scholar
10	Baer MR, Stewart CC, Lawrence D, et al: Acute myeloid leukemia with 11q23 translocations: myelomonocytic immunophenotype by multiparameter flow cytometry. Leukemia. 12:317–325. 1998. View Article : Google Scholar : PubMed/NCBI
11	Marukawa O, Akao Y, Inazawa J, et al: Molecular cloning of the breakpoint of t(11;22) (q23;q11) chromosome translocation in an adult acute myelomonocytic leukaemia. Br J Haematol. 92:687–691. 1996. View Article : Google Scholar : PubMed/NCBI
12	Kobayashi H, Miyachi H, Ogawa T and Jimbo M: Translocation t(11;22)(q23;q11) in an adult with acute monoblastic leukemia. Jpn J Med. 29:527–532. 1990. View Article : Google Scholar : PubMed/NCBI
13	Shaffer LG, Slovak ML and Campbell LJ: An International System for Human Cytogenetic Nomenclature. S. Karger; Basel: pp. 1382009