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Article

Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells

  • Authors:
    • Gulperi Oktem
    • Ayhan Bilir
    • Ruchan Uslu
    • Sevinc V. Inan
    • Sirin B. Demiray
    • Harika Atmaca
    • Sule Ayla
    • Ogun Sercan
    • Aysegul Uysal
  • View Affiliations / Copyright

    Affiliations: Department of Histology and Embryology, Faculty of Medicine, Ege University, Bornova, Izmir 35100, Turkey, Department of Histology and Embryology, Istanbul Medical Faculty, Istanbul University, Capa, Istanbul 34098, Turkey, Department of Medical Oncology, Faculty of Medicine, Ege University, Bornova, Izmir 35100, Turkey, Department of Histology and Embryology, Faculty of Medicine, Celal Bayar University, Manisa 45030, Turkey, Department of Biology, Faculty of Science and Art, Celal Bayar University, Manisa 45030, Turkey, Zeynep Kamil Gynecology and Maternity Training and Research Hospital, Istanbul 34668, Turkey, Department of Medical Biology, Faculty of Medicine, Dokuz Eylul University, Bornova, Izmir 35340, Turkey
  • Pages: 2103-2109
    |
    Published online on: March 24, 2014
       https://doi.org/10.3892/ol.2014.1992
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Abstract

Cancer stem cells (CSC) isolated from multiple tumor types differentiate in vivo and in vitro when cultured in serum; however, the factors responsible for their differentiation have not yet been identified. The first aim of the present study was to identify CD133high/CD44high DU145 prostate CSCs and compare their profiles with non‑CSCs as bulk counterparts of the population. Subsequently, the two populations continued to be three‑dimensional multicellular spheroids. Differentiation was then investigated with stem cell‑related genomic characteristics. Polymerase chain reaction array analyses of cell cycle regulation, embryonic and mesenchymal cell lineage‑related markers, and telomerase reverse transcriptase (TERT) and Notch signaling were performed. Immunohistochemistry of CD117, Notch1, Jagged1, Delta1, Sox2, c‑Myc, Oct4, KLF4, CD90 and SSEA1 were determined in CSC and non‑CSC monolayer and spheroid subcultures. Significant gene alterations were observed in the CD133high/CD44high population when cultured as a monolayer and continued as spheroid. In this group, marked gene upregulation was determined in collagen type 9 α1, Islet1 and cyclin D2. Jagged1, Delta‑like 3 and Notch1 were respectively upregulated genes in the Notch signaling pathway. According to immunoreactivity, the staining density of Jagged1, Sox2, Oct4 and Klf‑4 increased significantly in CSC spheroids. Isolated CSCs alter their cellular characterization over the course of time and exhibit a differentiation profile while maintaining their former surface antigens at a level of transcription or translation. The current study suggested that this differentiation process may be a mechanism responsible for the malignant process and tumor growth.
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1 

Clarke MF, Dick JE, Dirks PB, Eaves CJ, Jamieson CH, Jones DL, Visvader J, Weissman IL and Wahl GM: Cancer stem cells - perspectives on current status and future directions: AACR Workshop on cancer stem cells. Cancer Res. 66:9339–9344. 2006. View Article : Google Scholar

2 

Vermeulen L, Sprick MR, Kemper K, Stassi G and Medema JP: Cancer stem cells - old concepts, new insights. Cell Death Differ. 15:947–958. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Hill RP and Perris R: ‘Destemming’ cancer stem cells. J Natl Cancer Inst. 99:1435–1440. 2007.

4 

Miki J and Rhim JS: Prostate cell cultures as in vitro models for the study of normal stem cells and cancer stem cells. Prostate Cancer Prostatic Dis. 11:32–39. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Botchkina GI, Zuniga SZ, Das M, Wang Y, Wang H, Zhu S, Savitt AG, Rowehl RA, Leyfman Y, Ju J, et al: New-generation taxoid SB-T-1214 inhibits stem cell-related gene expression in 3D cancer spheroids induced by purified colon tumor-initiating cells. Mol Cancer. 9:1922010. View Article : Google Scholar : PubMed/NCBI

6 

Shackleton M, Quintana E, Fearon ER and Morrison SJ: Heterogeneity in cancer: cancer stem cells versus clonal evolution. Cell. 138:822–829. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Tang DG: Understanding cancer stem cell heterogeneity and plasticity. Cell Res. 22:457–472. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Li Y and Laterra J: Cancer stem cells: distinct entities or dynamically regulated phenotypes? Cancer Res. 72:576–580. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Singh SK, Clarke ID, Terasaki M, Bonn VE, Hawkins C, Squire J and Dirks PB: Identification of a cancer stem cell in human brain tumors. Cancer Res. 63:5821–5828. 2003.PubMed/NCBI

10 

Liu G, Yuan X, Zeng Z, Tunici P, Ng H, Abdulkadir IR, Lu L, Irvin D, Black KL and Yu JS: Analysis of gene expression and chemoresistance of CD133+ cancer stem cells in glioblastoma. Mol Cancer. 5:672006. View Article : Google Scholar : PubMed/NCBI

11 

Bussolati B, Bruno S, Grange C, Buttiglieri S, Deregibus MC, Cantino D and Camussi G: Isolation of renal progenitor cells from adult human kidney. Am J Pathol. 166:545–555. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Suetsugu A, Nagaki M, Aoki H, Motohashi T, Kunisada T and Moriwaki H: Characterization of CD133+ hepatocellular carcinoma cells as cancer stem/progenitor cells. Biochem and Biophys Res Commun. 351:820–824. 2006.

13 

Yin S, Li J, Hu C, Chen X, et al: CD133 positive hepatocellular carcinoma cells posess high capacity for tumorigenicity. Int J Cancer. 120:1444–1450. 2007. View Article : Google Scholar : PubMed/NCBI

14 

O’Brien CA, Pollett A, Gallinger S and Dick J: A human colon cancer cell capable of initiating timor growth in immunodeficient mice. Nature. 445:106–110. 2007.PubMed/NCBI

15 

Hermann PC, Huber SL, Herrler T, Aicher A, Ellwart JW, Guba M, Bruns CJ and Heeschen C: Distinct populations of cancer stem cells determine tumor growth and metastatic activity in human pancreatic cancer. Cell Stem Cell. 1:313–323. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Maitland NJ and Collins AT: Prostate cancer stem cells: a new target for therapy. J Clin Oncol. 26:2862–2870. 2008. View Article : Google Scholar

17 

Shmelkov SV, St Clair R, Lyden D and Rafii S: AC133/CD133/Prominin-1. Int J Biochem Cell Biol. 37:715–719. 2005. View Article : Google Scholar : PubMed/NCBI

18 

Shmelkov SV, Butler JM, Hooper AT, Hormigo A, Kushner J, Milde T, St Clair R, et al: CD133 expression is not restricted to stem cells, and both CD133+ and CD133− metastatic colon cancer cells initiate tumors. J Clin Invest. 118:2111–2120. 2008.PubMed/NCBI

19 

Beier D, Hau P, Proescholdt M, Lohmeier A, Wischhusen J, Oefner PJ, Aigner L, Brawanski A, Bogdahn U and Beier CP: CD133+ and CD133− glioblastoma-derived cancer stem cells show differential growth characteristics and molecular profiles. Cancer Res. 67:4010–4015. 2007.PubMed/NCBI

20 

Joo KM, Kim SY, Jin X, Song SY, Kong DS, Lee JI, Jeon JW, Kim MH, Kang BG, Jung Y, et al: Clinical and biological implications of CD133-positive and CD133-negative cells in glioblastomas. Lab Invest. 808:808–815. 2008.PubMed/NCBI

21 

Orian-Rousseau V: CD44, a therapeutic target for metastasising tumours. Eur J Cancer. 46:1271–1277. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Ponta H, Sherman L and Herrlich PA: CD44: from adhesion molecules to signaling regulators. Nat Rev Mol Cell Biol. 4:33–45. 2003. View Article : Google Scholar : PubMed/NCBI

23 

Ugolkov AV, Eisengart LJ, Luan C and Yang XJ: Expression analysis of putative stem cell markers in human benign and malignant prostate. Prostate. 71:18–25. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Collins AT, Berry PA, Hyde C, Stower MJ and Maitland NJ: Prospective identification of tumorigenic prostate cancer stem cells. Cancer Res. 65:10946–10951. 2005. View Article : Google Scholar : PubMed/NCBI

25 

Robertson FM, Ogasawara MA, Ye Z, Chu K, Pickei R, Debeb BG, Woodward WA, Hittelman WN, Cristofanilli M and Barsky SH: Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype. J Biomol Screen. 15:820–829. 2010. View Article : Google Scholar : PubMed/NCBI

26 

Acker H: Microenvironmental conditions in multicellular spheroids grown under liquid-overlay tissue culture conditions. Recent Results Cancer Res. 95:116–133. 1984. View Article : Google Scholar : PubMed/NCBI

27 

Oktem G, Sanci M, Bilir A, Yildirim Y, Kececi SD, Ayla S and Inan S: Cancer stem cell and embryonic development-associated molecules contribute to prognostic significance in ovarian cancer. Int J Gynecol Cancer. 22:23–29. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Lai EC: Notch signaling: control of cell communication and cell fate. Development. 131:965–973. 2004. View Article : Google Scholar : PubMed/NCBI

29 

Parsons P, Gilbert SJ, Vaughan-Thomas A, Sorrell DA, Notman R, Bishop M, Hayes AJ, Mason DJ and Duance VC: Type IX collogen interacts with fibronectin providing an important molecular bridge in articular cartilage. J Biol Chem. 286:34986972011. View Article : Google Scholar : PubMed/NCBI

30 

Huang CC, Wang TC, Lin BH, Wang YW, Johnson SL and Yu J: Collagen IX is required for the integrity of the collagen II fibrils and the regulation of vascular plexus formation in zebrafish caudalfins. Dev Biol. 332:360–370. 2009. View Article : Google Scholar : PubMed/NCBI

31 

Piotrowski A, Benetkiewicz M, Menzel U, et al: Microarray-based survey of CpG islands identifies concurrent hyper- and hypomethylation patterns in tissues derived from patients with breast cancer. Genes Chromosomes Cancer. 45:656–667. 2006. View Article : Google Scholar : PubMed/NCBI

32 

Schmitt AM, Riniker F, Anlauf M, Schmid S, Soltermann A, Moch H, Heitz PU, Klöppel G, Komminoth P and Perren A: Islet 1 (Isl1) expression is a reliable marker for pancreatic endocrine tumorsand their metasteses. Am J Surg Pathol. 32:420–425. 2008. View Article : Google Scholar : PubMed/NCBI

33 

Barzelay A, Ben-Shoshan J, Entin-Meer M, Maysel-Auslender S, Afek A, Barshack Keren G and George J: A potential role for islet-1 in post-natal angiogenesis and vasculogenesis. Thromb Haemost. 103:188–197. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Li JL and Harris AL: Crosstalk of VEGF and Notch pathways in tumour angiogenesis: therapeutic implications. Front Biosci. 14:3094–3110. 2009.PubMed/NCBI

35 

Sethi N, Dai X, Winter CG and Kang Y: Tumor-derived JAGGED1 promotes osteolytic bone metastasis of breast cancer by engaging notch signaling in bone cells. Cancer Cell. 19:192–205. 2011. View Article : Google Scholar : PubMed/NCBI

36 

Reedijk M, Odorcic S, Chang L, Zhang H, Miller N, McCready DR, et al: High-level coexpression of JAG1 and NOTCH1 is observed in human breast cancer and is associated with poor overall survival. Cancer Res. 65:8530–8537. 2005. View Article : Google Scholar : PubMed/NCBI

37 

Wu K, Xu L, Zhang L, Lin Z and Hou J: High Jagged1 expression predicts poor outcome in clear cell renal cell carcinoma. Jpn J Clin Oncol. 41:411–416. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Gao J, Liu J, Fan D, Xu H, Xiong Y, Wang Y, Xu W, Wang Y, Cheng Y and Zheng G: Up-regulated expression of Notch1 and Jagged1 in human colon adenocarcinoma. Pathol Biol (Paris). 59:298–302. 2010. View Article : Google Scholar : PubMed/NCBI

39 

Santagata S, Demichelis F, Riva A, Varambally S, Hofer MD, Kutok JL, et al: JAGGED1 expression is associated with prostate cancer metastasis and recurrence. Cancer Res. 64:6854–6857. 2004. View Article : Google Scholar : PubMed/NCBI

40 

Boscolo E, Stewart CL, Greenberger S, Wu JK, Durham JT, Herman IM, Mulliken JB, Kitajewski J and Bischoff J: JAGGED1 signaling regulates hemangioma stem cell-to pericyte/vascular smooth muscle cell differentiation. Arterioscler Thromb Vasc Biol. 31:2181–2192. 2011. View Article : Google Scholar : PubMed/NCBI

41 

Sharma G, Mirza S, Prasad CP, Srivastava A, Gupta SD and Ralhan R: Promoter hypermethylation of p16INK4A, p14ARF, CyclinD2 and Slit2 in serum and tumor DNA from breast cancer patients. Life Sci. 80:1873–1881. 2007. View Article : Google Scholar : PubMed/NCBI

42 

Henrique R, Costa VL, Cerveira N, Carvalho AL, Hoque MO, Ribeiro FR, Oliveira J, Teixeira MR, Sidransky D and Jeronimo C: Hypermethylation of Cyclin D2 is associated with loss of mRNA expression and tumor development in prostate cancer. J Mol Med. 84:911–918. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Oktem G, Bilir A, Uslu R, Inan SV, Demiray SB, Atmaca H, Ayla S, Sercan O and Uysal A: Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells. Oncol Lett 7: 2103-2109, 2014.
APA
Oktem, G., Bilir, A., Uslu, R., Inan, S.V., Demiray, S.B., Atmaca, H. ... Uysal, A. (2014). Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells. Oncology Letters, 7, 2103-2109. https://doi.org/10.3892/ol.2014.1992
MLA
Oktem, G., Bilir, A., Uslu, R., Inan, S. V., Demiray, S. B., Atmaca, H., Ayla, S., Sercan, O., Uysal, A."Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells". Oncology Letters 7.6 (2014): 2103-2109.
Chicago
Oktem, G., Bilir, A., Uslu, R., Inan, S. V., Demiray, S. B., Atmaca, H., Ayla, S., Sercan, O., Uysal, A."Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells". Oncology Letters 7, no. 6 (2014): 2103-2109. https://doi.org/10.3892/ol.2014.1992
Copy and paste a formatted citation
x
Spandidos Publications style
Oktem G, Bilir A, Uslu R, Inan SV, Demiray SB, Atmaca H, Ayla S, Sercan O and Uysal A: Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells. Oncol Lett 7: 2103-2109, 2014.
APA
Oktem, G., Bilir, A., Uslu, R., Inan, S.V., Demiray, S.B., Atmaca, H. ... Uysal, A. (2014). Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells. Oncology Letters, 7, 2103-2109. https://doi.org/10.3892/ol.2014.1992
MLA
Oktem, G., Bilir, A., Uslu, R., Inan, S. V., Demiray, S. B., Atmaca, H., Ayla, S., Sercan, O., Uysal, A."Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells". Oncology Letters 7.6 (2014): 2103-2109.
Chicago
Oktem, G., Bilir, A., Uslu, R., Inan, S. V., Demiray, S. B., Atmaca, H., Ayla, S., Sercan, O., Uysal, A."Expression profiling of stem cell signaling alters with spheroid formation in CD133high/CD44high prostate cancer stem cells". Oncology Letters 7, no. 6 (2014): 2103-2109. https://doi.org/10.3892/ol.2014.1992
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