Involvement of RbAp48 in erythroid differentiation of murine erythroleukemia cells induced by sodium butyrate

Qi,Wu‑Lin; Cao,Ling‑Ling; Hu,Jiang‑Jiang; Xue,Jian‑You; Sang,Ting‑Ting; Zheng,Ya‑Juan; Chen,Tao; Wang,Jie; Zhao,Fu‑Kun; Zhang,Shi‑Fu

doi:10.3892/ol.2014.2015

June-2014 Volume 7 Issue 6

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June-2014 Volume 7 Issue 6

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Involvement of RbAp48 in erythroid differentiation of murine erythroleukemia cells induced by sodium butyrate

Authors:
- Wu‑Lin Qi
- Ling‑Ling Cao
- Jiang‑Jiang Hu
- Jian‑You Xue
- Ting‑Ting Sang
- Ya‑Juan Zheng
- Tao Chen
- Jie Wang
- Fu‑Kun Zhao
- Shi‑Fu Zhang
View Affiliations / Copyright

Affiliations: College of Life Sciences, Zhejiang Sci‑Tech University, Hangzhou, Zhejiang 310018, P.R. China
Pages: 1785-1789
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Published online on: March 31, 2014

https://doi.org/10.3892/ol.2014.2015
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Abstract

Normal mammalian terminal erythroid differentiation is a precisely regulated process during which the progenitor cells execute particular programs to form a mature erythrocytic phenotype. In the present study, it was found that RbAp48, a histone‑binding protein associated with retinoblastoma protein, was upregulated during terminal erythroid maturation in vivo and in vitro. This indicated that RbAp48, at least in part, participated in the regulation of murine erythropoiesis. Following sodium butyrate (SB) induction, murine erythroleukemia (MEL) cells began to re‑enter erythroid differentiation and the ratio of differentiated cells reached ~80% at 72 h. The erythroid maturation‑related mRNA expression of α‑globin, β‑globin and glycophorin A (GPA) was increased markedly, which indicated that SB induced MEL differentiation. During MEL differentiation, the RbAp48 level showed a 1.5‑fold increase at 72 h, and the globin transcription factor (GATA)‑1 level was also upregulated in the early stage of differentiation. By contrast, the c‑Myc level was gradually downregulated in MEL differentiation. Using an immunofluorescence assay, the results of the study directly showed that the average fluorescence intensity of RbAp48 in each cell reached an almost 1.7‑fold increase at 72 and 96 h. This was consistent with the western blot results of RbAp48 during MEL differentiation. In addition, reduced expression of RbAp48 by RNA inference decreased SB‑induced MEL differentiation by ~20%, indicating that a high level of RbAp48 was essential for MEL differentiation. Taken together, these results established a functional link between RbAp48 and erythroid differentiation.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Involvement of RbAp48 in erythroid differentiation of murine erythroleukemia cells induced by sodium butyrate

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