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Article

FBXL20 acts as an invasion inducer and mediates E‑cadherin in colorectal adenocarcinoma

  • Authors:
    • Jianjun Zhu
    • Shishan Deng
    • Jie Duan
    • Xingguo Xie
    • Shiquan Xu
    • Maocheng Ran
    • Xiaosi Dai
    • Yu Pu
    • Xiaoming Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Human Anatomy, Premedical College, North Sichuan Medical College, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Department of Neurosurgery, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China, Sichuan Key Laboratory of Medical Imaging, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, P.R. China
  • Pages: 2185-2191
    |
    Published online on: April 3, 2014
       https://doi.org/10.3892/ol.2014.2031
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Abstract

The mechanisms eliciting colorectal adenocarcinoma are not well understood and the FBXL20 gene is problematic as it exhibits an abnormal expression in colorectal cancer cells. In the present study a recombinant plasmid, pReceiver‑M03‑FBL20 expression plasmid was constructed, which overexpressed FBXL20; this was transfected into Lovo cells to form Lovo‑FBL20 cells. The FBXL20 expression level was examined by quantitative polymerase chain reaction (qPCR) and western blot analysis. The cell viability and invasion capacity were measured using cell counting kit 8, Transwell chamber and wound healing assays, respectively. The associated genes, including E‑cadherin, β‑catenin, c‑Myc, SET nuclear oncogene, protein phosphatase‑2A, Axin, p53 and caspase 3, were detected by qPCR and western blotting. It was demonstrated that the FBXL20 expression level was markedly upregulated in the Lovo‑FBL20 cells transfected with pReceiver‑M03‑FBL20 expression plasmid, compared with that of the Lovo cells. In addition, the cell viability and invasion capacity of the Lovo‑FBL20 cells were significantly increased. These increases correlated with a significant upregulation in the expression level of β‑catenin and c‑Myc, and a downregulated expression level of E‑cadherin. The results of the present study indicate that FBXL20 may mediate the ubiquitin degradation of E‑cadherin resulting in an increased invasive ability of malignant cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhu J, Deng S, Duan J, Xie X, Xu S, Ran M, Dai X, Pu Y and Zhang X: FBXL20 acts as an invasion inducer and mediates E‑cadherin in colorectal adenocarcinoma. Oncol Lett 7: 2185-2191, 2014.
APA
Zhu, J., Deng, S., Duan, J., Xie, X., Xu, S., Ran, M. ... Zhang, X. (2014). FBXL20 acts as an invasion inducer and mediates E‑cadherin in colorectal adenocarcinoma. Oncology Letters, 7, 2185-2191. https://doi.org/10.3892/ol.2014.2031
MLA
Zhu, J., Deng, S., Duan, J., Xie, X., Xu, S., Ran, M., Dai, X., Pu, Y., Zhang, X."FBXL20 acts as an invasion inducer and mediates E‑cadherin in colorectal adenocarcinoma". Oncology Letters 7.6 (2014): 2185-2191.
Chicago
Zhu, J., Deng, S., Duan, J., Xie, X., Xu, S., Ran, M., Dai, X., Pu, Y., Zhang, X."FBXL20 acts as an invasion inducer and mediates E‑cadherin in colorectal adenocarcinoma". Oncology Letters 7, no. 6 (2014): 2185-2191. https://doi.org/10.3892/ol.2014.2031
Copy and paste a formatted citation
x
Spandidos Publications style
Zhu J, Deng S, Duan J, Xie X, Xu S, Ran M, Dai X, Pu Y and Zhang X: FBXL20 acts as an invasion inducer and mediates E‑cadherin in colorectal adenocarcinoma. Oncol Lett 7: 2185-2191, 2014.
APA
Zhu, J., Deng, S., Duan, J., Xie, X., Xu, S., Ran, M. ... Zhang, X. (2014). FBXL20 acts as an invasion inducer and mediates E‑cadherin in colorectal adenocarcinoma. Oncology Letters, 7, 2185-2191. https://doi.org/10.3892/ol.2014.2031
MLA
Zhu, J., Deng, S., Duan, J., Xie, X., Xu, S., Ran, M., Dai, X., Pu, Y., Zhang, X."FBXL20 acts as an invasion inducer and mediates E‑cadherin in colorectal adenocarcinoma". Oncology Letters 7.6 (2014): 2185-2191.
Chicago
Zhu, J., Deng, S., Duan, J., Xie, X., Xu, S., Ran, M., Dai, X., Pu, Y., Zhang, X."FBXL20 acts as an invasion inducer and mediates E‑cadherin in colorectal adenocarcinoma". Oncology Letters 7, no. 6 (2014): 2185-2191. https://doi.org/10.3892/ol.2014.2031
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