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Article

A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion

  • Authors:
    • Jinhua Li
    • Xianzhong Yu
    • Thomas E. Wagner
    • Yanzhang Wei
  • View Affiliations / Copyright

    Affiliations: Department of Biotechnology, Greenville Technical College, Greenville, SC 29601, USA, Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA, Orbis Health Solutions, LLC., Greenville, SC 29601, USA
  • Pages: 198-202
    |
    Published online on: April 15, 2014
       https://doi.org/10.3892/ol.2014.2067
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Abstract

Although the generation of hybrid cells by cell fusion plays a significant role in biotechnology and biomedicine, the low cell‑fusion rates and the limitation of large‑scale cell fusion for clinical applications of the two widely used approaches, polyethylene‑glycol (PEG)‑mediated cell fusion and electrofusion, hinder the application of this critical technology in certain key areas, including cancer immunotherapy. In the present study, a simple procedure that can not only significantly increase the heterologous cell fusion but is also capable of producing fused cells on a large scale is reported. A biotin‑streptavidin‑biotin (BSB) bridge was created by coating one to‑be‑fused cell with biotin and the other with biotin‑streptavidin. The BSB bridge enhances cell‑fusion rates induced with PEG fusion or electrofusion by 10‑30% depending on the cell types when compared with cell fusions without the bridge. The procedure described increases heterologous cell pairing and eliminates the alignment step required for the majority of electrofusions. Notably, it can be used to make large‑scale cell fusions for clinical applications.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Yu X, Wagner TE and Wei Y: A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion. Oncol Lett 8: 198-202, 2014.
APA
Li, J., Yu, X., Wagner, T.E., & Wei, Y. (2014). A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion. Oncology Letters, 8, 198-202. https://doi.org/10.3892/ol.2014.2067
MLA
Li, J., Yu, X., Wagner, T. E., Wei, Y."A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion". Oncology Letters 8.1 (2014): 198-202.
Chicago
Li, J., Yu, X., Wagner, T. E., Wei, Y."A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion". Oncology Letters 8, no. 1 (2014): 198-202. https://doi.org/10.3892/ol.2014.2067
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Yu X, Wagner TE and Wei Y: A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion. Oncol Lett 8: 198-202, 2014.
APA
Li, J., Yu, X., Wagner, T.E., & Wei, Y. (2014). A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion. Oncology Letters, 8, 198-202. https://doi.org/10.3892/ol.2014.2067
MLA
Li, J., Yu, X., Wagner, T. E., Wei, Y."A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion". Oncology Letters 8.1 (2014): 198-202.
Chicago
Li, J., Yu, X., Wagner, T. E., Wei, Y."A biotin‑streptavidin‑biotin bridge dramatically enhances cell fusion". Oncology Letters 8, no. 1 (2014): 198-202. https://doi.org/10.3892/ol.2014.2067
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