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Article

Transcription factor Snai1‑1 induces osteosarcoma invasion and metastasis by inhibiting E‑cadherin expression

  • Authors:
    • Huiguang Yang
    • Yunqing Zhang
    • Zhengming Zhou
    • Xuefeng Jiang
    • Aidong Shen
  • View Affiliations / Copyright

    Affiliations: Department of Orthopaedics, The Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin, Jiangsu 214400, P.R. China
  • Pages: 193-197
    |
    Published online on: April 22, 2014
       https://doi.org/10.3892/ol.2014.2079
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Abstract

Osteosarcoma (OS) is a type of primary malignant bone tumor with a high propensity for local recurrence and distant metastasis. A previous study showed Snail‑1 is highly expressed in OS cells. The present study aimed to investigate the association between the transcription factor Snai1 and E‑cadherin in OS. SaOS2 OS cells were transfected either with a plasmid expressing short hairpin RNA (shRNA) specific for the Snai1‑1 gene (SaOS2‑shRNA) or a negative control plasmid (SaOS2‑Mock). The expression levels of E‑cadherin and Snai1‑1 in the transfected and control cells were determined by quantitative polymerase chain reaction and western blot analysis. In addition, the study was extended to evaluate the migratory and invasive properties of the cells through a Transwell experiment. The results show that E‑cadherin was expressed at a high level in the SaOS2‑shRNA cells, which were much less migratory and invasive than the control cells. Overexpression of Snai1‑1 in OS is associated with tumor progression, possibly through the suppression of E‑cadherin expression and induction of the process of epithelial‑mesenchymal transition, which contributes to the proceeding invasion and metastasis of OS cells.
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Copy and paste a formatted citation
Spandidos Publications style
Yang H, Zhang Y, Zhou Z, Jiang X and Shen A: Transcription factor Snai1‑1 induces osteosarcoma invasion and metastasis by inhibiting E‑cadherin expression. Oncol Lett 8: 193-197, 2014.
APA
Yang, H., Zhang, Y., Zhou, Z., Jiang, X., & Shen, A. (2014). Transcription factor Snai1‑1 induces osteosarcoma invasion and metastasis by inhibiting E‑cadherin expression. Oncology Letters, 8, 193-197. https://doi.org/10.3892/ol.2014.2079
MLA
Yang, H., Zhang, Y., Zhou, Z., Jiang, X., Shen, A."Transcription factor Snai1‑1 induces osteosarcoma invasion and metastasis by inhibiting E‑cadherin expression". Oncology Letters 8.1 (2014): 193-197.
Chicago
Yang, H., Zhang, Y., Zhou, Z., Jiang, X., Shen, A."Transcription factor Snai1‑1 induces osteosarcoma invasion and metastasis by inhibiting E‑cadherin expression". Oncology Letters 8, no. 1 (2014): 193-197. https://doi.org/10.3892/ol.2014.2079
Copy and paste a formatted citation
x
Spandidos Publications style
Yang H, Zhang Y, Zhou Z, Jiang X and Shen A: Transcription factor Snai1‑1 induces osteosarcoma invasion and metastasis by inhibiting E‑cadherin expression. Oncol Lett 8: 193-197, 2014.
APA
Yang, H., Zhang, Y., Zhou, Z., Jiang, X., & Shen, A. (2014). Transcription factor Snai1‑1 induces osteosarcoma invasion and metastasis by inhibiting E‑cadherin expression. Oncology Letters, 8, 193-197. https://doi.org/10.3892/ol.2014.2079
MLA
Yang, H., Zhang, Y., Zhou, Z., Jiang, X., Shen, A."Transcription factor Snai1‑1 induces osteosarcoma invasion and metastasis by inhibiting E‑cadherin expression". Oncology Letters 8.1 (2014): 193-197.
Chicago
Yang, H., Zhang, Y., Zhou, Z., Jiang, X., Shen, A."Transcription factor Snai1‑1 induces osteosarcoma invasion and metastasis by inhibiting E‑cadherin expression". Oncology Letters 8, no. 1 (2014): 193-197. https://doi.org/10.3892/ol.2014.2079
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