Open Access

EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells

  • Authors:
    • Peijie Chen
    • Yan Huang
    • Bo Zhang
    • Qiuquan Wang
    • Peiming Bai
  • View Affiliations

  • Published online on: April 25, 2014     https://doi.org/10.3892/ol.2014.2093
  • Pages: 41-46
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Abstract

EphA2 is persistently overexpressed and functionally changed in numerous human cancers. However, to the best of our knowledge, the role that EphA2 plays in prostate cancer is not entirely clear. To investigate the roles of EphA2 in the development and progression of prostate cancer, the present study initially evaluated the roles of the EphA2 protein in LNCaP prostate cancer cells using recombinant plasmid, western blot analysis, flow cytometry, Matrigel invasion chamber and the cell counting kit‑8 assay. An immunohistochemistry assay was also conducted to observe the effects of EphA2 in prostate cancer tissues. The results demonstrated that the LNCaP human prostate cancer cells that were transfected with pcDNA3.1(+) plasmid‑mediated pcDNA3.1(+)‑EphA2, markedly enhanced the cell growth and invasion in vitro. Additionally, EphA2 was overexpressed in prostate cancer specimens and the expression of EphA2 was significantly associated with Gleason grade, total prostate‑specific antigen, advanced clinical stage and lymph node metastasis. Collectively, these results demonstrate that EphA2 is involved in malignant cell behavior and is a potential therapeutic target in human prostate cancer.
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July-2014
Volume 8 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
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Spandidos Publications style
Chen P, Huang Y, Zhang B, Wang Q and Bai P: EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells. Oncol Lett 8: 41-46, 2014.
APA
Chen, P., Huang, Y., Zhang, B., Wang, Q., & Bai, P. (2014). EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells. Oncology Letters, 8, 41-46. https://doi.org/10.3892/ol.2014.2093
MLA
Chen, P., Huang, Y., Zhang, B., Wang, Q., Bai, P."EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells". Oncology Letters 8.1 (2014): 41-46.
Chicago
Chen, P., Huang, Y., Zhang, B., Wang, Q., Bai, P."EphA2 enhances the proliferation and invasion ability of LNCaP prostate cancer cells". Oncology Letters 8, no. 1 (2014): 41-46. https://doi.org/10.3892/ol.2014.2093