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Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer

  • Authors:
    • Deniz Abat
    • Osman Demirhan
    • Nihal Inandiklioglu
    • Erdal Tunc
    • Seyda Erdogan
    • Deniz Tastemir
    • Inayet Nur Uslu
    • Zuhtu Tansug
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey, Department of Medical Biology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey, Department of Pathology, Faculty of Medicine, Çukurova University, 01330 Adana, Turkey, Vocational School of Health Services, Adıyaman University, 02040 Adıyaman, Turkey
  • Pages: 25-32
    |
    Published online on: May 2, 2014
       https://doi.org/10.3892/ol.2014.2108
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Abstract

A majority of patients with bladder cancer present with superficial disease and subsequently, some patients show progression to muscle invasive or metastatic disease. Bladder cancer has a complex genetic process and identification of the genetic alterations which occur during progression may lead to the understanding of the nature of the disease and provide the possibility of early treatment. The aim of the present study was to compare the structural and numerical chromosomal differences and changes in the p16 and p53 genes between low‑grade (LG) and high‑grade (HG) bladder cancer (BC) using cytogenetic and molecular cytogenetic methods. Between March 2009 and March 2010, cytogenetic analyses were carried out on tumor and blood samples in 34 patients with transitional cell type BC, and on blood samples of 34 healthy patients as a control group. Fluorescence in situ hybridization probes for the p16 and p53 genes were also used to screen the alterations in these genes in 32 patients with BC. The patients were divided into two groups (LG and HG) and the findings were compared. A total of 11 (32.3%) patients exhibited LGBC, 22 (64.7%) exhibited HGBC and one (3%) patient exhibited carcinoma in situ. There were no differences between the LGBC and HGBC groups according to the number of chromosomal aberrations (P=0.714); however, differences between alterations of the p16 and p53 genes were significant (P=0.002 and P=0.039). Almost all structural abnormalities were found to be located to the 1q21, 1q32, 3p21 and 5q31 regions in patients with HG tumors. In conclusion, the p16 and p53 genes were altered more prominently in patients with HG tumors compared with LG tumors. The structural abnormalities in the 1q21, 1q32, 3p21 and 5q31 regions were observed more frequently in patients with HG tumors. These regions may play significant roles in the progression of BC, but further studies are required to find candidate genes for a panel of BC.
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Copy and paste a formatted citation
Spandidos Publications style
Abat D, Demirhan O, Inandiklioglu N, Tunc E, Erdogan S, Tastemir D, Uslu IN and Tansug Z: Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer. Oncol Lett 8: 25-32, 2014.
APA
Abat, D., Demirhan, O., Inandiklioglu, N., Tunc, E., Erdogan, S., Tastemir, D. ... Tansug, Z. (2014). Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer. Oncology Letters, 8, 25-32. https://doi.org/10.3892/ol.2014.2108
MLA
Abat, D., Demirhan, O., Inandiklioglu, N., Tunc, E., Erdogan, S., Tastemir, D., Uslu, I. N., Tansug, Z."Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer". Oncology Letters 8.1 (2014): 25-32.
Chicago
Abat, D., Demirhan, O., Inandiklioglu, N., Tunc, E., Erdogan, S., Tastemir, D., Uslu, I. N., Tansug, Z."Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer". Oncology Letters 8, no. 1 (2014): 25-32. https://doi.org/10.3892/ol.2014.2108
Copy and paste a formatted citation
x
Spandidos Publications style
Abat D, Demirhan O, Inandiklioglu N, Tunc E, Erdogan S, Tastemir D, Uslu IN and Tansug Z: Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer. Oncol Lett 8: 25-32, 2014.
APA
Abat, D., Demirhan, O., Inandiklioglu, N., Tunc, E., Erdogan, S., Tastemir, D. ... Tansug, Z. (2014). Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer. Oncology Letters, 8, 25-32. https://doi.org/10.3892/ol.2014.2108
MLA
Abat, D., Demirhan, O., Inandiklioglu, N., Tunc, E., Erdogan, S., Tastemir, D., Uslu, I. N., Tansug, Z."Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer". Oncology Letters 8.1 (2014): 25-32.
Chicago
Abat, D., Demirhan, O., Inandiklioglu, N., Tunc, E., Erdogan, S., Tastemir, D., Uslu, I. N., Tansug, Z."Genetic alterations of chromosomes, p53 and p16 genes in low- and high-grade bladder cancer". Oncology Letters 8, no. 1 (2014): 25-32. https://doi.org/10.3892/ol.2014.2108
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