A novel cyclic pentapeptide, H‑10, inhibits B16 cancer cell growth and induces cell apoptosis

Zhang,Geng; Liu,Shouxin; Liu,Yunjiang; Wang,Feifei; Ren,Jingwen; Gu,Jifeng; Zhou,Kaixuan; Shan,Baoen

doi:10.3892/ol.2014.2121

July-2014 Volume 8 Issue 1

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July-2014 Volume 8 Issue 1

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Article

A novel cyclic pentapeptide, H‑10, inhibits B16 cancer cell growth and induces cell apoptosis

Authors:
- Geng Zhang
- Shouxin Liu
- Yunjiang Liu
- Feifei Wang
- Jingwen Ren
- Jifeng Gu
- Kaixuan Zhou
- Baoen Shan
View Affiliations / Copyright

Affiliations: Research Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China, State Key Laboratory Breeding Base‑Hebei Province Key Laboratory of Molecular Chemistry for Drug, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, P.R. China, Breast Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
Pages: 248-252
|
Published online on: May 8, 2014

https://doi.org/10.3892/ol.2014.2121
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Abstract

Sansalvamide A, a cyclic depsipeptide isolated from a marine fungus of the Fusarium genus, exhibits significant antitumor activity. In the present study, H‑10 (molecular formula, C38H55N5O6; molecular weight, 677.8732), a novel sansalvamide A derivative, demonstrated an inhibitory effect on the proliferation of murine melanoma B16 cells. It was confirmed that H‑10 induced the apoptosis of the B16 cells. The inhibitory rate of various concentrations of H‑10 on the B16 cells was measured by sulforhodamine B colorimetric assay, and the results revealed that the inhibitory effect of H‑10 on the B16 cells occurred in a concentration‑dependent manner. In addition, a growth curve model of the B16 cells treated with 50 µM H‑10 revealed that the effect of H‑10 also occurred in a time‑dependent manner. The apoptotic morphology of the B16 cells was observed using an optical microscope. Following the treatment of the cells with 50 µM H‑10 for 24 h, the cell apoptosis rate was analyzed using flow cytometry. The expression levels of caspase‑3, ‑8 and ‑9 were analyzed by western blot analysis, and the results indicated that H‑10 may induce the apoptosis of B16 cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

A novel cyclic pentapeptide, H‑10, inhibits B16 cancer cell growth and induces cell apoptosis

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Abstract

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