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Article

Antitumor activity of G‑quadruplex‑interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells

  • Authors:
    • Hongli Liu
    • Changshuai Lv
    • Baijuan Ding
    • Jie Wang
    • Shan Li
    • Youzhong Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China
  • Pages: 409-413
    |
    Published online on: May 8, 2014
       https://doi.org/10.3892/ol.2014.2125
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Abstract

The aim of the present study was to investigate the potential effects of photodynamic therapy (PDT) mediated by the cationic porphyrin, 5,10,15,20‑tetra‑(N‑methyl‑4‑pyridyl)porphine (TMPyP4), on an ovarian carcinoma cell line and the underlying mechanisms by which TMPyP4‑PDT exerts its actions. The analysis of cell viability, hematoxylin and eosin staining and flow cytometric apoptosis assays revealed that TMPyP4‑PDT potently suppressed the growth of the A2780 cells in a laser energy‑ and dose‑dependent manner. Mechanically, it was observed that TMPyP4‑PDT suppressed the proliferation and motility of the A2780 cells. In addition, the expression levels of minichromosome maintenance protein‑2 (MCM2) and carbonic anhydrase IX (CA‑IX) were detected by western blot analysis. The results indicated that the TMPyP4‑PDT‑induced apoptosis and antimetastatic activity in the A2780 cells was accompanied by the inhibition of the expression of MCM2 and CA‑IX. Therefore, TMPyP4‑PDT may represent a potential therapeutic method for the treatment of ovarian carcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Lv C, Ding B, Wang J, Li S and Zhang Y: Antitumor activity of G‑quadruplex‑interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells. Oncol Lett 8: 409-413, 2014.
APA
Liu, H., Lv, C., Ding, B., Wang, J., Li, S., & Zhang, Y. (2014). Antitumor activity of G‑quadruplex‑interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells. Oncology Letters, 8, 409-413. https://doi.org/10.3892/ol.2014.2125
MLA
Liu, H., Lv, C., Ding, B., Wang, J., Li, S., Zhang, Y."Antitumor activity of G‑quadruplex‑interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells". Oncology Letters 8.1 (2014): 409-413.
Chicago
Liu, H., Lv, C., Ding, B., Wang, J., Li, S., Zhang, Y."Antitumor activity of G‑quadruplex‑interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells". Oncology Letters 8, no. 1 (2014): 409-413. https://doi.org/10.3892/ol.2014.2125
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Lv C, Ding B, Wang J, Li S and Zhang Y: Antitumor activity of G‑quadruplex‑interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells. Oncol Lett 8: 409-413, 2014.
APA
Liu, H., Lv, C., Ding, B., Wang, J., Li, S., & Zhang, Y. (2014). Antitumor activity of G‑quadruplex‑interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells. Oncology Letters, 8, 409-413. https://doi.org/10.3892/ol.2014.2125
MLA
Liu, H., Lv, C., Ding, B., Wang, J., Li, S., Zhang, Y."Antitumor activity of G‑quadruplex‑interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells". Oncology Letters 8.1 (2014): 409-413.
Chicago
Liu, H., Lv, C., Ding, B., Wang, J., Li, S., Zhang, Y."Antitumor activity of G‑quadruplex‑interactive agent TMPyP4 with photodynamic therapy in ovarian carcinoma cells". Oncology Letters 8, no. 1 (2014): 409-413. https://doi.org/10.3892/ol.2014.2125
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