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Article

Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR‑106

  • Authors:
    • Peng Zhang
    • Ling Dong
    • Hua Long
    • Tong‑Tao Yang
    • Yong Zhou
    • Qing‑Yu Fan
    • Bao‑An Ma
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China, Department of Physiology, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China
  • Pages: 127-132
    |
    Published online on: May 8, 2014
       https://doi.org/10.3892/ol.2014.2127
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Abstract

Osteosarcoma (OS) is the most frequent primary bone sarcoma and tends to develop pulmonary metastasis. Studies have shown that mesenchymal stem cells (MSCs) are involved in OS growth and metastasis, but the mechanism remains unclear. The aim of the present study was to identify whether homologous MSCs could promote the growth and metastasis of OS in rats with a normal immune system. The OS cell line, UMR‑106, which originally derives from a Sprague‑Dawley (SD) rat‑transplantable osteogenic sarcoma with an osteoblastic phenotype, has a strong carcinogenic capability and a high lung metastasis. Xenotransplanted models of UMR‑106 with or without MSCs injected through the tibia (IT) or caudal vein (IV) were established. SD rats were randomly divided into six groups: Control, UMR‑106 (IT), MSCs (IV), UMR‑106 (IT) + MSCs (IV), UMR‑106 (IV) and UMR‑106 (IV) + MSCs (IV). Following injection, all rats were sacrificed at week 5, and the volume and quantity of metastatic sarcoma and the serum alkaline phosphatase levels were measured. There was no metastatic sarcoma in the liver, spleen and kidney in all groups. The rats in the MSCs (IV) + UMR‑106 (IV) group showed a significantly higher volume and number of pulmonary metastatic tumors than those of the UMR‑106 (IV) group. In pulmonary metastatic tissues, MSCs were found in the MSCs (IV) + UMR‑106 (IV) group, but not in the UMR‑106 (IT) + MSCs (IV) group. Notably, the expression of vascular endothelial growth factor (VEGF) was increased in the MSCs + UMR‑106 cells co‑culture system. The present study indicated that MSCs can significantly promote the pulmonary metastasis of the rat OS cell line, UMR‑106, with a normal immune system, and VEGF was involved in MSC‑promoted UMR‑106 emergence and growth of pulmonary metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang P, Dong L, Long H, Yang TT, Zhou Y, Fan QY and Ma BA: Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR‑106. Oncol Lett 8: 127-132, 2014.
APA
Zhang, P., Dong, L., Long, H., Yang, T., Zhou, Y., Fan, Q., & Ma, B. (2014). Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR‑106. Oncology Letters, 8, 127-132. https://doi.org/10.3892/ol.2014.2127
MLA
Zhang, P., Dong, L., Long, H., Yang, T., Zhou, Y., Fan, Q., Ma, B."Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR‑106". Oncology Letters 8.1 (2014): 127-132.
Chicago
Zhang, P., Dong, L., Long, H., Yang, T., Zhou, Y., Fan, Q., Ma, B."Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR‑106". Oncology Letters 8, no. 1 (2014): 127-132. https://doi.org/10.3892/ol.2014.2127
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang P, Dong L, Long H, Yang TT, Zhou Y, Fan QY and Ma BA: Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR‑106. Oncol Lett 8: 127-132, 2014.
APA
Zhang, P., Dong, L., Long, H., Yang, T., Zhou, Y., Fan, Q., & Ma, B. (2014). Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR‑106. Oncology Letters, 8, 127-132. https://doi.org/10.3892/ol.2014.2127
MLA
Zhang, P., Dong, L., Long, H., Yang, T., Zhou, Y., Fan, Q., Ma, B."Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR‑106". Oncology Letters 8.1 (2014): 127-132.
Chicago
Zhang, P., Dong, L., Long, H., Yang, T., Zhou, Y., Fan, Q., Ma, B."Homologous mesenchymal stem cells promote the emergence and growth of pulmonary metastases of the rat osteosarcoma cell line UMR‑106". Oncology Letters 8, no. 1 (2014): 127-132. https://doi.org/10.3892/ol.2014.2127
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