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Article

Epithelial cell adhesion molecule is overexpressed in hypopharyngeal carcinoma and suppresses the metastasis and proliferation of the disease when downregulated

  • Authors:
    • Yakui Mu
    • Na Sa
    • Liang Yu
    • Sumei Lu
    • Haibo Wang
    • Wei Xu
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology‑Head and Neck Surgery, Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China, Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Jinan, Shandong 250014, P.R. China
  • Pages: 175-182
    |
    Published online on: May 13, 2014
       https://doi.org/10.3892/ol.2014.2140
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Abstract

The epithelial cell adhesion molecule (EpCAM) is overexpressed in the majority of human epithelial carcinomas, and its overexpression is associated with proliferation and neoplastic transformation. However, the precise molecular mechanism involved in EpCAM‑related proliferation and metastasis in hypopharyngeal carcinoma is unknown. The aim of the present study was to identify the role of EpCAM in the metastasis and proliferation of hypopharyngeal carcinoma. An immunohistochemical staining assay indicated that EpCAM was overexpressed in primary hypopharyngeal carcinoma tissues, and that this overexpression correlated with the tumor size and lymph node metastasis. In the following treatment of the hypopharyngeal carcinoma FaDu cell line with EpCAM, the downregulation of EpCAM was found to significantly suppress cell metastasis and proliferation, as detected by Transwell, clone formation and MTT assays. Additionally, western blot analysis revealed that EpCAM downregulation increased the expression of the adhesion‑ and proliferation‑related factors, E‑cadherin, α‑catenin and β‑catenin, in the cytoskeleton, as well as β‑catenin expression in the nucleus. In conclusion, the present study indicated that EpCAM is a potential oncogene and contributes to the metastasis of hypopharyngeal carcinoma. The current study is the first to provide evidence for the potential value of targeting EpCAM in hypopharyngeal carcinoma therapy.
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1 

Clark JI, Hofmeister C, Choudhury A, et al: Phase II evaluation of paclitaxel in combination with carboplatin in advanced head and neck carcinoma. Cancer. 92:2334–2340. 2001.

2 

Hoffman HT, Porter K, Karnell LH, et al: Laryngeal cancer in the United States: changes in demographics, patterns of care, and survival. Laryngoscope. 116(Suppl 111): S1–S13. 2006.

3 

Ma J, Lu S, Yu L, et al: FaDu cell characteristics induced by multidrug resistance. Oncol Rep. 26:1189–1195. 2011.

4 

Baeuerle PA and Gires O: EpCAM (CD326) finding its role in cancer. Br J Cancer. 96:417–423. 2007.

5 

Schnell U, Cirulli V and Giepmans BN: EpCAM: structure and function in health and disease. Biochim Biophys Acta. 1828:1989–2001. 2013.

6 

Went P, Vasei M, Bubendorf L, et al: Frequent high-level expression of the immunotherapeutic target Ep-CAM in colon, stomach, prostate and lung cancers. Br J Cancer. 94:128–135. 2006.

7 

Osta WA, Chen Y, Mikhitarian K, et al: EpCAM is overexpressed in breast cancer and is a potential target for breast cancer gene therapy. Cancer Res. 64:5818–5824. 2004.

8 

Flatmark K, Borgen E, Nesland JM, et al: Disseminated tumour cells as a prognostic biomarker in colorectal cancer. Br J Cancer. 104:1434–1439. 2011.

9 

Ni J, Cozzi PJ, Duan W, et al: Role of the EpCAM (CD326) in prostate cancer metastasis and progression. Cancer Metastasis Rev. 31:779–791. 2012.

10 

Ji J, Yamashita T, Budhu A, et al: Identification of microRNA-181 by genome-wide screening as a critical player in EpCAM-positive hepatic cancer stem cells. Hepatology. 50:472–480. 2009.

11 

Pauli C, Münz M, Kieu C, et al: Tumor-specific glycosylation of the carcinoma-associated epithelial cell adhesion molecule EpCAM in head and neck carcinomas. Cancer Lett. 193:25–32. 2003.

12 

Litvinov SV, Velders MP, Bakker HA, Fleuren GJ and Warnaar SO: Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol. 125:437–446. 1994.

13 

Balzar M, Prins FA, Bakker HA, Fleuren GJ, Warnaar SO and Litvinov SV: The structural analysis of adhesions mediated by Ep-CAM. Exp Cell Res. 246:108–121. 1999.

14 

Winter MJ, Nagelkerken B, Mertens AE, Rees-Bakker HA, Briaire-de Bruijn IH and Litvinov SV: Expression of Ep-CAM shifts the state of cadherin-mediated adhesions from strong to weak. Exp Cell Res. 285:50–58. 2003.

15 

Maetzel D, Denzel S, Mack B, et al: Nuclear signalling by tumour-associated antigen EpCAM. Nat Cell Biol. 11:162–171. 2009.

16 

Visvader JE and Lindeman GJ: Cancer stem cells in solid tumours: accumulating evidence and unresolved questions. Nat Rev Cancer. 8:755–768. 2008.

17 

Gires O, Klein CA and Baeuerle PA: On the abundance of EpCAM on cancer stem cells. Nat Rev Cancer. 9:1432009.

18 

van der Gun BT, Melchers LJ, Ruiters MH, de Leij LF, McLaughlin PM and Rots MG: EpCAM in carcinogenesis: the good, the bad or the ugly. Carcinogenesis. 31:1913–1921. 2010.

19 

Kroepil F, Dulian A, Vallböhmer D, et al: High EpCAM expression is linked to proliferation and lauren classification in gastric cancer. BMC Res Notes. 6:2532013.

20 

Yanamoto S, Kawasaki G, Yoshitomi I, Iwamoto T, Hirata K and Mizuno A: Clinicopathologic significance of EpCAM expression in squamous cell carcinoma of the tongue and its possibility as a potential target for tongue cancer gene therapy. Oral Oncol. 43:869–877. 2007.

21 

Litvinov SV, Bakker HA, Gourevitch MM, Velders MP and Warnaar SO: Evidence for a role of the epithelial glycoprotein 40 (Ep-CAM) in epithelial cell-cell adhesion. Cell Adhes Commun. 2:417–428. 1994.

22 

Dufour S, Mège RM and Thiery JP: α-catenin, vinculin, and F-actin in strengthening E-cadherin cell-cell adhesions and mechanosensing. Cell Adh Migr. 7:345–350. 2013.

23 

Chaves-Pérez A, Mack B, Maetzel D, et al: EpCAM regulates cell cycle progression via control of cyclin D1 expression. Oncogene. 32:641–650. 2013.

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Copy and paste a formatted citation
Spandidos Publications style
Mu Y, Sa N, Yu L, Lu S, Wang H and Xu W: Epithelial cell adhesion molecule is overexpressed in hypopharyngeal carcinoma and suppresses the metastasis and proliferation of the disease when downregulated. Oncol Lett 8: 175-182, 2014.
APA
Mu, Y., Sa, N., Yu, L., Lu, S., Wang, H., & Xu, W. (2014). Epithelial cell adhesion molecule is overexpressed in hypopharyngeal carcinoma and suppresses the metastasis and proliferation of the disease when downregulated. Oncology Letters, 8, 175-182. https://doi.org/10.3892/ol.2014.2140
MLA
Mu, Y., Sa, N., Yu, L., Lu, S., Wang, H., Xu, W."Epithelial cell adhesion molecule is overexpressed in hypopharyngeal carcinoma and suppresses the metastasis and proliferation of the disease when downregulated". Oncology Letters 8.1 (2014): 175-182.
Chicago
Mu, Y., Sa, N., Yu, L., Lu, S., Wang, H., Xu, W."Epithelial cell adhesion molecule is overexpressed in hypopharyngeal carcinoma and suppresses the metastasis and proliferation of the disease when downregulated". Oncology Letters 8, no. 1 (2014): 175-182. https://doi.org/10.3892/ol.2014.2140
Copy and paste a formatted citation
x
Spandidos Publications style
Mu Y, Sa N, Yu L, Lu S, Wang H and Xu W: Epithelial cell adhesion molecule is overexpressed in hypopharyngeal carcinoma and suppresses the metastasis and proliferation of the disease when downregulated. Oncol Lett 8: 175-182, 2014.
APA
Mu, Y., Sa, N., Yu, L., Lu, S., Wang, H., & Xu, W. (2014). Epithelial cell adhesion molecule is overexpressed in hypopharyngeal carcinoma and suppresses the metastasis and proliferation of the disease when downregulated. Oncology Letters, 8, 175-182. https://doi.org/10.3892/ol.2014.2140
MLA
Mu, Y., Sa, N., Yu, L., Lu, S., Wang, H., Xu, W."Epithelial cell adhesion molecule is overexpressed in hypopharyngeal carcinoma and suppresses the metastasis and proliferation of the disease when downregulated". Oncology Letters 8.1 (2014): 175-182.
Chicago
Mu, Y., Sa, N., Yu, L., Lu, S., Wang, H., Xu, W."Epithelial cell adhesion molecule is overexpressed in hypopharyngeal carcinoma and suppresses the metastasis and proliferation of the disease when downregulated". Oncology Letters 8, no. 1 (2014): 175-182. https://doi.org/10.3892/ol.2014.2140
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