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Article

Biological characteristics of prostate cancer cells are regulated by hypoxia‑inducible factor 1α

  • Authors:
    • Xiang Huang
    • Jianhua Zhou
    • Junyan Liu
    • Binzhi Tang
    • Fengyan Zhao
    • Yi Qu
  • View Affiliations / Copyright

    Affiliations: Department of Urology, Sichuan Academy of Medical Sciences and Sichuan Provincial People's Hospital, Chengdu, Sichuan 610041, P.R. China, Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 1217-1221
    |
    Published online on: June 17, 2014
       https://doi.org/10.3892/ol.2014.2259
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Abstract

Hypoxia‑inducible factor (HIF)‑1α has been reported to be associated with malignancy in a number of types of cancer. However, the role of HIF‑1 α in the regulation of prostate cancer (PCa) growth has yet to be elucidated. The present study aimed to investigate the effect of HIF‑1α on the biological characteristics of the PCa PC3 cell line. Full‑length (fL) HIF‑1α and dominant‑negative (dn) HIF‑1α were transfected into PC3 cells. The expression of HIF‑1α and its downstream genes, including vascular endothelial growth factor (VEGF), erythropoietin (EPO) and CXC chemokine receptor 4 (CXCR4), were detected using western blot analysis. Cell proliferation, apoptosis and migration were assessed using MTT, terminal deoxynucleotidyl transferase‑mediated dUTP nick end labeling and Boyden chamber assays. The expression of VEGF, EPO and CXCR4 was found to be upregulated in the fL HIF‑1α‑transfected PC3 cells and downregulated in the dn HIF‑1α‑transfected PC3 cells. The overexpression of HIF‑1α was observed to enhance cell proliferation and migration and decrease docetaxol‑induced cell apoptosis. However, dn HIF‑1α was found to attenuate cell proliferation and migration, and promote docetaxol‑induced cell apoptosis. These findings indicate that HIF‑1α regulates the proliferation, apoptosis and migration of PC3 cells, at least in part, by regulating the expression of its target genes, including VEGF, EPO and CXCR4. Thus, the use of HIF‑1α inhibitors may be a promising therapy for the treatment of PCa.
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1 

Fukuda R, Zhang H, Kim JW, et al: HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells. Cell. 129:111–122. 2007.

2 

Hirota K and Semenza GL: Regulation of angiogenesis by hypoxia-inducible factor 1. Crit Rev Oncol Hematol. 59:15–26. 2006.

3 

Kilic M, Kasperczyk H, Fulda S and Debatin KM: Role of hypoxia inducible factor-1 alpha in modulation of apoptosis resistance. Oncogene. 26:2027–2038. 2007.

4 

Esteban MA, Tran MG, Harten SK, et al: Regulation of E-cadherin expression by VHL and hypoxia-inducible factor. Cancer Res. 66:3567–3575. 2006.

5 

Li J, Shi M, Cao Y, et al: Knockdown of hypoxia-inducible factor-1alpha in breast carcinoma MCF-7 cells results in reduced tumor growth and increased sensitivity to methotrexate. Biochem Biophys Res Commun. 342:1341–1351. 2006.

6 

Huang LE, Gu J, Schau M and Bunn HF: Regulation of hypoxia-inducible factor 1alpha is mediated by an O2-dependent degradation domain via the ubiquitin-proteasome pathway. Proc Natl Acad Sci USA. 95:7987–7992. 1998.

7 

Stiehl DP, Jelkmann W, Wenger RH and Hellwig-Bürgel T: Normoxic induction of the hypoxia-inducible factor 1alpha by insulin and interleukin-1beta involves the phosphatidylinositol 3-kinase pathway. FEBS Lett. 512:157–162. 2002.

8 

Semenza G: Signal transduction to hypoxia-inducible factor 1. Biochem Pharmacol. 64:993–998. 2002.

9 

Bárdos JI and Ashcrof M: Negative and positive regulation of HIF-1: a complex network. Biochim Biophys Acta. 1755:107–120. 2005.

10 

Zhong H, De Marzo AM, Laughner E, et al: Overexpression of hypoxia-inducible factor 1alpha in common human cancers and their metastases. Cancer Res. 59:5830–5835. 1999.

11 

Maxwell PH, Pugh CW and Ratcliffe PJ: Activation of the HIF pathway in cancer. Curr Opin Genet Dev. 11:293–299. 2001.

12 

Semenza GL: Targeting HIF-1 for cancer therapy. Nat Rev Cancer. 3:721–732. 2003.

13 

Semenza GL: Hypoxia-inducible factors: mediators of cancer progression and targets for cancer therapy. Trends Pharmacol Sci. 33:207–214. 2012.

14 

Chen J, Zhao S, Nakada K, et al: Dominant-negative hypoxia-inducible factor-1 alpha reduces tumorigenicity of pancreatic cancer cells through the suppression of glucose metabolism. Am J Pathol. 162:1283–1291. 2003.

15 

Perrot-Applanat M and Di Benedetto M: Autocrine functions of VEGF in breast tumor cells: adhesion, survival, migration and invasion. Cell Adh Migr. 6:547–553. 2012.

16 

Elliott S and Sinclair AM: The effect of erythropoietin on normal and neoplastic cells. Biologics. 6:163–189. 2012.

17 

Zheng H, Fu G, Dai T and Huang H: Migration of endothelial progenitor cells mediated by stromal cell-derived factor-1alpha/CXCR4 via PI3K/Akt/eNOS signal transduction pathway. J Cardiovasc Pharmacol. 50:274–280. 2007.

18 

Merseburger AS, Bellmunt J, Jenkins C, et al: Perspectives on treatment of metastatic castration-resistant prostate cancer. Oncologist. 18:558–567. 2013.

19 

Zhang P, Wang Y, Hui Y, et al: Inhibition of VEGF expression by targeting HIF-1alpha with small interference RNA in human RPE cells. Ophthalmologica. 221:411–417. 2007.

20 

Melillo G: Inhibiting hypoxia-inducible factor 1 for cancer therapy. Mol Cancer Res. 4:601–605. 2006.

21 

Jensen RL, Ragel BT, Whang K and Gillespie D: Inhibition of hypoxia inducible factor-1alpha (HIF-1alpha) decreases vascular endothelial growth factor (VEGF) secretion and tumor growth in malignant gliomas. J Neurooncol. 78:233–247. 2006.

22 

Stoeltzing O, McCarty MF, Wey JS, et al: Role of hypoxia-inducible factor 1alpha in gastric cancer cell growth, angiogenesis, and vessel maturation. J Natl Cancer Inst. 96:946–956. 2004.

23 

Brown LM, Cowen RL, Debray C, et al: Reversing hypoxic cell chemoresistance in vitro using genetic and small molecule approaches targeting hypoxia inducible factor-1. Mol Pharmacol. 69:411–418. 2006.

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Copy and paste a formatted citation
Spandidos Publications style
Huang X, Zhou J, Liu J, Tang B, Zhao F and Qu Y: Biological characteristics of prostate cancer cells are regulated by hypoxia‑inducible factor 1α. Oncol Lett 8: 1217-1221, 2014.
APA
Huang, X., Zhou, J., Liu, J., Tang, B., Zhao, F., & Qu, Y. (2014). Biological characteristics of prostate cancer cells are regulated by hypoxia‑inducible factor 1α. Oncology Letters, 8, 1217-1221. https://doi.org/10.3892/ol.2014.2259
MLA
Huang, X., Zhou, J., Liu, J., Tang, B., Zhao, F., Qu, Y."Biological characteristics of prostate cancer cells are regulated by hypoxia‑inducible factor 1α". Oncology Letters 8.3 (2014): 1217-1221.
Chicago
Huang, X., Zhou, J., Liu, J., Tang, B., Zhao, F., Qu, Y."Biological characteristics of prostate cancer cells are regulated by hypoxia‑inducible factor 1α". Oncology Letters 8, no. 3 (2014): 1217-1221. https://doi.org/10.3892/ol.2014.2259
Copy and paste a formatted citation
x
Spandidos Publications style
Huang X, Zhou J, Liu J, Tang B, Zhao F and Qu Y: Biological characteristics of prostate cancer cells are regulated by hypoxia‑inducible factor 1α. Oncol Lett 8: 1217-1221, 2014.
APA
Huang, X., Zhou, J., Liu, J., Tang, B., Zhao, F., & Qu, Y. (2014). Biological characteristics of prostate cancer cells are regulated by hypoxia‑inducible factor 1α. Oncology Letters, 8, 1217-1221. https://doi.org/10.3892/ol.2014.2259
MLA
Huang, X., Zhou, J., Liu, J., Tang, B., Zhao, F., Qu, Y."Biological characteristics of prostate cancer cells are regulated by hypoxia‑inducible factor 1α". Oncology Letters 8.3 (2014): 1217-1221.
Chicago
Huang, X., Zhou, J., Liu, J., Tang, B., Zhao, F., Qu, Y."Biological characteristics of prostate cancer cells are regulated by hypoxia‑inducible factor 1α". Oncology Letters 8, no. 3 (2014): 1217-1221. https://doi.org/10.3892/ol.2014.2259
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