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Article Open Access

Shikonin promotes autophagy in BXPC‑3 human pancreatic cancer cells through the PI3K/Akt signaling pathway

  • Authors:
    • Shuqing Shi
    • Haimei Cao
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China
  • Pages: 1087-1089
    |
    Published online on: June 26, 2014
       https://doi.org/10.3892/ol.2014.2293
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Abstract

The present study aimed to investigate the effect of shikonin on autophagy in BXPC‑3 human pancreatic cancer cells and its underlying mechanism. Cell viability was assessed using the Cell Counting Kit‑8 assay and the expression of light chain (LC) 3, p62, phosphoinositide 3‑kinase (PI3K), Akt, phosphorylated (p)‑PI3K and p‑Akt was analyzed using western blot analysis. Following treatment with 1 µmol/l shikonin for 48 h and 2.5 and 5 µmol/l shikonin for 24 and 48 h, the viability of the BXPC‑3 cells was found to be significantly reduced and the protein expression of LC3‑II/LC3‑I was observed to be increased, while the protein expression of p62, PI3K, Akt, p‑PI3K and p‑Akt was decreased. These findings suggest that shikonin promotes autophagy in BXPC‑3 cells and that the underlying mechanism may be associated with the PI3K/Akt signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Shi S and Cao H: Shikonin promotes autophagy in BXPC‑3 human pancreatic cancer cells through the PI3K/Akt signaling pathway. Oncol Lett 8: 1087-1089, 2014.
APA
Shi, S., & Cao, H. (2014). Shikonin promotes autophagy in BXPC‑3 human pancreatic cancer cells through the PI3K/Akt signaling pathway. Oncology Letters, 8, 1087-1089. https://doi.org/10.3892/ol.2014.2293
MLA
Shi, S., Cao, H."Shikonin promotes autophagy in BXPC‑3 human pancreatic cancer cells through the PI3K/Akt signaling pathway". Oncology Letters 8.3 (2014): 1087-1089.
Chicago
Shi, S., Cao, H."Shikonin promotes autophagy in BXPC‑3 human pancreatic cancer cells through the PI3K/Akt signaling pathway". Oncology Letters 8, no. 3 (2014): 1087-1089. https://doi.org/10.3892/ol.2014.2293
Copy and paste a formatted citation
x
Spandidos Publications style
Shi S and Cao H: Shikonin promotes autophagy in BXPC‑3 human pancreatic cancer cells through the PI3K/Akt signaling pathway. Oncol Lett 8: 1087-1089, 2014.
APA
Shi, S., & Cao, H. (2014). Shikonin promotes autophagy in BXPC‑3 human pancreatic cancer cells through the PI3K/Akt signaling pathway. Oncology Letters, 8, 1087-1089. https://doi.org/10.3892/ol.2014.2293
MLA
Shi, S., Cao, H."Shikonin promotes autophagy in BXPC‑3 human pancreatic cancer cells through the PI3K/Akt signaling pathway". Oncology Letters 8.3 (2014): 1087-1089.
Chicago
Shi, S., Cao, H."Shikonin promotes autophagy in BXPC‑3 human pancreatic cancer cells through the PI3K/Akt signaling pathway". Oncology Letters 8, no. 3 (2014): 1087-1089. https://doi.org/10.3892/ol.2014.2293
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