Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression

Hwang,Tsann‑Long; Changchien,Tzu‑Tsung; Wang,Chee‑Chan; Wu,Chi‑Ming

doi:10.3892/ol.2014.2295

Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression

Authors:
- Tsann‑Long Hwang
- Tzu‑Tsung Changchien
- Chee‑Chan Wang
- Chi‑Ming Wu
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Affiliations: Department of Surgery, Chang Gung Memorial Hospital, Tao‑Yuan 33305, Taiwan, R.O.C., Department of Cosmetic Science, Vanung University, Tao‑Yuan 32061, Taiwan, R.O.C.
Published online on: June 27, 2014 https://doi.org/10.3892/ol.2014.2295
Pages: 1367-1371

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Abstract

Claudin‑4 is a member of a large family of transmembrane proteins known as claudins, which are essential for the formation and maintenance of tight junctions. Our previous studies have revealed that claudin‑4 proteins are overexpressed in metastatic gastric cancer. To clarify the roles of claudin‑4 in gastric cancer metastasis, human gastric adenocarcinoma (AGS) cells constitutively expressing wild‑type claudin‑4 were generated. Expression of claudin‑4 in AGS cells was found to increase cell invasion and migration, as measured by Boyden invasion chamber assays. Moreover, the claudin‑4‑expressing AGS cells were found to have increased matrix metalloproteinase (MMP)‑2 and ‑9 expression, indicating that claudin‑mediated increased invasion may be mediated through the activation of the MMP protein. Overall, the results suggest that claudin‑4 overexpression may promote gastric cancer metastasis through the increased invasion of gastric cancer cells.

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