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Article

Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression

  • Authors:
    • Tsann‑Long Hwang
    • Tzu‑Tsung Changchien
    • Chee‑Chan Wang
    • Chi‑Ming Wu
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Chang Gung Memorial Hospital, Tao‑Yuan 33305, Taiwan, R.O.C., Department of Cosmetic Science, Vanung University, Tao‑Yuan 32061, Taiwan, R.O.C.
  • Pages: 1367-1371
    |
    Published online on: June 27, 2014
       https://doi.org/10.3892/ol.2014.2295
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Abstract

Claudin‑4 is a member of a large family of transmembrane proteins known as claudins, which are essential for the formation and maintenance of tight junctions. Our previous studies have revealed that claudin‑4 proteins are overexpressed in metastatic gastric cancer. To clarify the roles of claudin‑4 in gastric cancer metastasis, human gastric adenocarcinoma (AGS) cells constitutively expressing wild‑type claudin‑4 were generated. Expression of claudin‑4 in AGS cells was found to increase cell invasion and migration, as measured by Boyden invasion chamber assays. Moreover, the claudin‑4‑expressing AGS cells were found to have increased matrix metalloproteinase (MMP)‑2 and ‑9 expression, indicating that claudin‑mediated increased invasion may be mediated through the activation of the MMP protein. Overall, the results suggest that claudin‑4 overexpression may promote gastric cancer metastasis through the increased invasion of gastric cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Hwang TL, Changchien TT, Wang CC and Wu CM: Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression. Oncol Lett 8: 1367-1371, 2014.
APA
Hwang, T., Changchien, T., Wang, C., & Wu, C. (2014). Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression. Oncology Letters, 8, 1367-1371. https://doi.org/10.3892/ol.2014.2295
MLA
Hwang, T., Changchien, T., Wang, C., Wu, C."Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression". Oncology Letters 8.3 (2014): 1367-1371.
Chicago
Hwang, T., Changchien, T., Wang, C., Wu, C."Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression". Oncology Letters 8, no. 3 (2014): 1367-1371. https://doi.org/10.3892/ol.2014.2295
Copy and paste a formatted citation
x
Spandidos Publications style
Hwang TL, Changchien TT, Wang CC and Wu CM: Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression. Oncol Lett 8: 1367-1371, 2014.
APA
Hwang, T., Changchien, T., Wang, C., & Wu, C. (2014). Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression. Oncology Letters, 8, 1367-1371. https://doi.org/10.3892/ol.2014.2295
MLA
Hwang, T., Changchien, T., Wang, C., Wu, C."Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression". Oncology Letters 8.3 (2014): 1367-1371.
Chicago
Hwang, T., Changchien, T., Wang, C., Wu, C."Claudin‑4 expression in gastric cancer cells enhances the invasion and is associated with the increased level of matrix metalloproteinase‑2 and ‑9 expression". Oncology Letters 8, no. 3 (2014): 1367-1371. https://doi.org/10.3892/ol.2014.2295
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