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Article

Synergy of 1,25‑dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV‑3 cells

  • Authors:
    • Zengli Zhang
    • Hemei Zhang
    • Zhiyong Hu
    • Ping Wang
    • Jianmei Wan
    • Bingyan Li
  • View Affiliations / Copyright

    Affiliations: Department of Toxicology, School of Public Health, Soochow University, Suzhou, Jiangsu 215123, P.R. China, Li Shui Center for Disease Control and Prevention, Lishui, Zhejiang 323000, P.R. China
  • Pages: 1348-1354
    |
    Published online on: July 2, 2014
       https://doi.org/10.3892/ol.2014.2307
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Abstract

1α,25-Dihydroxyvitamin D3 [1,25(OH)2D3] has been demonstrated to inhibit the growth of cancer cells. However, carboplatin is the most widely used chemotherapeutic agent to treat cancer. We hypothesized that vitamin D may enhance the antiproliferative effects of carboplatin, and tested this hypothesis in ovarian cancer SKOV‑3 cells treated with carboplatin and 1,25(OH)2D3. Cell viability was determined by Cell Counting Kit‑8, while cell cycle distribution, apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) were analyzed by flow cytometry. In these experiments, 1,25(OH)2D3 and carboplatin each provided dose‑dependent suppression of SKOV‑3 growth, and synergy was demonstrated between 10 nM 1,25(OH)2D3 and carboplatin. The proportion of cells in G0/G1 phase was markedly reduced by the drug combination, while the proportion of cells in G2/M phase was increased. Apoptosis did not increase in ovarian cancer cells treated with 10 nM 1,25(OH)2D3 alone; however, 1,25(OH)2D3 evidently enhanced carboplatin‑induced apoptosis. Similarly, ROS production was evidently higher and MMP was lower in cells treated with the two drugs than in those treated with each drug alone. The results suggested that 1,25(OH)2D3 suppresses SKOV‑3 growth and enhances the antiproliferative effect of carboplatin. The drugs function synergistically by inducing cell cycle arrest, increasing apoptosis and ROS production, and reducing MMP.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Zhang H, Hu Z, Wang P, Wan J and Li B: Synergy of 1,25‑dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV‑3 cells. Oncol Lett 8: 1348-1354, 2014.
APA
Zhang, Z., Zhang, H., Hu, Z., Wang, P., Wan, J., & Li, B. (2014). Synergy of 1,25‑dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV‑3 cells. Oncology Letters, 8, 1348-1354. https://doi.org/10.3892/ol.2014.2307
MLA
Zhang, Z., Zhang, H., Hu, Z., Wang, P., Wan, J., Li, B."Synergy of 1,25‑dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV‑3 cells". Oncology Letters 8.3 (2014): 1348-1354.
Chicago
Zhang, Z., Zhang, H., Hu, Z., Wang, P., Wan, J., Li, B."Synergy of 1,25‑dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV‑3 cells". Oncology Letters 8, no. 3 (2014): 1348-1354. https://doi.org/10.3892/ol.2014.2307
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Zhang H, Hu Z, Wang P, Wan J and Li B: Synergy of 1,25‑dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV‑3 cells. Oncol Lett 8: 1348-1354, 2014.
APA
Zhang, Z., Zhang, H., Hu, Z., Wang, P., Wan, J., & Li, B. (2014). Synergy of 1,25‑dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV‑3 cells. Oncology Letters, 8, 1348-1354. https://doi.org/10.3892/ol.2014.2307
MLA
Zhang, Z., Zhang, H., Hu, Z., Wang, P., Wan, J., Li, B."Synergy of 1,25‑dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV‑3 cells". Oncology Letters 8.3 (2014): 1348-1354.
Chicago
Zhang, Z., Zhang, H., Hu, Z., Wang, P., Wan, J., Li, B."Synergy of 1,25‑dihydroxyvitamin D3 and carboplatin in growth suppression of SKOV‑3 cells". Oncology Letters 8, no. 3 (2014): 1348-1354. https://doi.org/10.3892/ol.2014.2307
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