miR‑449a and CDK6 in gastric carcinoma
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- Published online on: July 22, 2014 https://doi.org/10.3892/ol.2014.2370
- Pages: 1533-1538
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Abstract
The present study aimed to identify the association between microRNA (miR/miRNA)‑449a, the cyclin‑dependent kinase (CDK)6 protein and gastric carcinoma, and discuss the effect of miR‑449a on the expression of the CDK6 protein. Quantitative (q)PCR and western blot analysis were used to analyze the expression of the miR‑449a and the CDK6 protein in gastric carcinoma and tumor‑adjacent normal tissues. The real‑time cell analyzer and the DAPI staining test were used to monitor the different miR‑449a levels regulating the proliferation and apoptosis of the MGC‑803 cell line. Immunofluorescence and western blot analyses were used to detect the expression level of the CDK6 protein in the cells of the miR‑449a upregulation and downregulation groups, and a control group. A scratch test was used to study the effects of miR‑449a expression on migration and invasion. It was found that the expression of miR‑449a was downregulated and the expression of CDK6 protein was upregulated in gastric carcinoma tissue. The level of MGC‑803 cell proliferation was decreased and the apoptosis level was increased by the upregulation of miR‑449a expression, and the opposite effect was shown by the downregulation of expression. The expression of the CDK6 protein in the MGC‑803 cells was downregulated by upregulating the expression of miR‑449a. The distance of the scratch was shortened markedly after 12 h by downregulating the expression of miR‑449a in the MGC‑803 cells. The present study identified that a lower expression level of miR‑449 and a higher expression level of CDK6 may contribute to the occurrence and development of gastric cancer. Furthermore, it was shown that miR‑449a is able to regulate the expression of the CDK6 protein.
