Analysis of TMEM174 gene expression in various renal cancer types by RNA in situ hybridization
- Authors:
- Published online on: July 30, 2014 https://doi.org/10.3892/ol.2014.2393
- Pages: 1693-1696
Abstract
Introduction
Transmembrane protein 174 (TMEM174) is a type III transmembrane protein that lacks a clear signal peptide. The N and C terminals are located inside of the cell. TMEM174 was originally identified from a large gene pool by high-throughput cell screening technology. This technique is used for the isolation of functional genes and for the analysis into the mechanisms of gene function (1). Dependent on its transmembrane helices, TMME174 overexpression is able to promote the transcriptional activity of activator protein 1 (AP-1), which is partly mediated by the ERK pathway (2). Our previous study demonstrated high TMEM174 expression in the kidney and also revealed its potential involvement with renal cancer based on its capacity to stimulate cell proliferation (2). The kidney is vital for the maintenance of the salt and water balance within the body, in order to keep a stable internal environment, i.e., for homeostasis. However, the expression of TMEM174 in renal cancer and normal renal tissues remains to be elucidated. In the present study, RNA in situ hybridization was used to detect the TMEM174 gene expression in various malignant renal cancer and normal renal tissues. The aim of this study was to provide a theoretical basis for the molecular mechanisms of the development of kidney cancer.
Materials and methods
Tissue microarray
Tissue microarrays were purchased from Shaanxi Chao Ying Biotechnology, Ltd., Co. (Xi’an, China). Information on each specimen, consisting of patient age, gender, organization, pathological diagnosis, clinical grade, tumor-node-metastasis classification, clinical stage, specimen type and result information, was available. TNM staging, clinical staging and pathological grading were determined based on the American Joint Committee on Cancer manual (3). Specimens were used from a total of 208 cases, including 178 cases of renal cancer and nephritis, 20 adjacent tissues and 10 normal tissues.
Preparation of digoxigenin-labeled probes for RNA in situ hybridization
The sense and anti-sense probes that matched the TMEM174 core responding sequence were as follows: Anti-sense, 5′-GAGCATTGTGTTATTATATCAG*AATA GCCTCTAGCGAGGGAGAGAGTATATT-3′DIG and sense, 5′-ATATACTCTCTCCCTCGCTAGAGGC*TATTCTGATA TAATAACACAATGCTCA-3′DIG. The asterisks indicate that the 3′ terminals were labeled with digoxigenin. All probes were synthesized by Shanghai Sangon Biological Engineering Technology and Services Co., Ltd. (Shanghai, China).
RNA in situ hybridization
Hybridization procedures were performed in this study with the RNA&ISH kit (KD2084, Roche Diagnostics, Indianapolis, IN, USA), based on the manufacturer’s instructions, with certain modifications. The glassware was washed, rinsed in distilled deionized water and autoclaved prior to use. Gloves were worn when the glassware and slides were handled to prevent RNase contamination of the tissue. The hybridization conditions were as follows: Probe concentration, 10 ng/μl; antibody titer, 1:400; washing temperature, room temperarture; dyeing temperature, 37°C; and dyeing time, 2 h. Deparaffinized sections were mounted on Denhardt-coated glass slides (D2532; Sigma Aldrich, St. Louis, MO, USA) and treated with pepsin (0.25 mg/ml in DEPC H2O-HCl; Sigma Aldrich) for 30 min in a 37°C water bath. The treated sections were then processed for in situ hybridization at 42–47°C for 24 h. The hybridization mixture contained the labeled oligonucleotide probe, 50% formamide, 10 mmol/l Tris-HCl, 1 mmol/l vanadyl-ribonucleoside complex (94740; Sigma Aldrich), 1 mmol/l CTAB (855820; pH 7.0; Sigma Aldrich), 0.15 mol/l Nacl, 1 mmol/l EDTA (pH 7.0), 1X Denhardt’s mixture and 10% dextran sulfate. Subsequent to hybridization, the slides were washed three times, for 30 min each time, in 0.1 mol/l Tris-buffered saline (TBS) at room temperature. The slides were then treated with TBS [100 mmol/l Tris (pH 7.5) and 150 mmol/l NaCl] containing a 1% blocking reagent (Roche Diagnostics, Shanghai, China) and 0.03% Triton X-100 for 30 min at room temperature, and incubated for 30 min with antidioxigenin alkaline phosphatase-conjugated antibodies (Roche Diagnostics) diluted at 1:4,000 in TBS containing 0.03% Triton X-100 and a 1% blocking reagent. Subsequent to being washed three times, for 15 min each time in TBS and 0.05% Tween 20, the slides were rinsed in a diammonimum phosphate (DAP)-buffer [100 mmol/l Tris (pH 9.5) 100 mmol/l NaCl, 50 mmol/l MgCl2] and hybridization signals were subsequently visualized using nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate as substrates [DAP-buffer (100 mmol/l Tris, pH 9.5, 100 mmol/l NaCl and 50 mmol/l MgCl2) in 10% PVA (341584; Sigma Aldrich)].
Results
Association between TMEM174 expression and renal pathological cell types
TMEM174 gene expression in the various malignant renal cancer and normal renal tissues was detected by RNA in situ hybridization. As shown in Table I and Fig. 1, TMEM174 exhibits differential expression in renal tissues. TMEM174 had a high positive rate of expression in squamous cell carcinoma with necrosis, papillary renal cell carcinoma and transitional cell carcinoma, and a low positive rate of expression in clear cell carcinoma, interstitial nephritis, undifferentiated carcinoma, retroperitoneal metastatic clear cell carcinoma, adrenal gland metastatic clear cell carcinoma, pelvic cavity metastatic chromophobe carcinoma, severe atypical hyperplasia of transitional epithelium and hyperplasia. Extremely weak expression was observe in collecting duct carcinoma, Wilms’ tumor, chronic pyelonephritis, acute pyelonephritis, cancer adjacent normal renal tissue and normal renal tissue.
Association between TMEM174 expression and renal pathological clinical stage
The association between TMEM174 expression and renal pathological clinical stage was analyzed. As shown in Table II, the THEM174 expression rate was 58% in phase I tissue samples, 50% in phase II tissue samples, 71% in phase III tissue samples and 50% in phase IV tissue samples.
Discussion
Each year in the United States, nearly 55,000 individuals are diagnosed with kidney cancer. In order to be effectively treated, an early diagnosis and effective surgical therapy are required. In more advanced-stage cases with involvement of the renal vein and lymph nodes or invasion through the renal cortex, surgical therapy frequently fails (4–8). Kidney cancer cell progression is a coordinated process that comprises cell cycle dysregulation and a specific gene expression program to determine tissue identity (9). The TMEM174 gene is highly expressed in the kidney tissues.
The AP-1 transcription factor is a heterodimeric protein formed from proteins of the c-Fos, ATF, c-Jun and JDP families. In response to a range of stimuli, including cytokines, stress, growth factors and viral and bacterial infections, AP-1 regulates gene expression (10). The cellular transcription factor cAMP-response element binding protein (CREB) binds to specific DNA sequences referred to as cAMP response elements (CREs), thereby regulating downstream gene transcription (11). TMEM174 overexpression has been shown to enhance the transcriptional activity of AP-1 and promote cell proliferation (2). In addition, our recent studies demonstrated that the CREB and AP-1 transcription factors are involved in the transcriptional regulation of the TMME174 gene (12).
When antibodies are not available, RNA in situ hybridization is a useful method that allows the determination of the transcriptional expression pattern of a gene (13). With this technique, the expression of multiple RNA species may be assayed using distinct RNA-labeled probes, or the RNA and protein localization within larval tissues may be examined. In the present study, RNA in situ hybridization analysis was used to detect the expression of TMEM174, and the results showed that its expression is varies among differing renal tissues. These results indicate that TMEM174 may have a significant role in the development of renal cancer.
Acknowledgements
This study was supported by grants from the National Natural Science Foundation of China (nos. 81072093, 30671092 and 81302323) and the Natural Science Foundation of Hebei Province (nos. C2009001260, C2014209140 and C2013209024).
