Analysis of TMEM174 gene expression in various renal cancer types by RNA in situ hybridization

Zhang,Xiujun; Hu,Fen; Meng,Lijun; Gou,Lixia; Luo,Mengmeng

doi:10.3892/ol.2014.2393

October 2014 Volume 8 Issue 4

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October 2014 Volume 8 Issue 4

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Article

Analysis of TMEM174 gene expression in various renal cancer types by RNA in situ hybridization

Authors:
- Xiujun Zhang
- Fen Hu
- Lijun Meng
- Lixia Gou
- Mengmeng Luo
View Affiliations / Copyright

Affiliations: College of Life Sciences, Hebei United University, Tangshan, Hebei 063000, P.R. China, Department of Environment and Chemical Engineering, Tangshan College, Tangshan, Hebei 063000, P.R. China
Pages: 1693-1696
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Published online on: July 30, 2014

https://doi.org/10.3892/ol.2014.2393
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Abstract

Transmembrane protein 174 (TMEM174) mRNA is easily detectable in human kidney tissues and activates AP‑1 and promotes 293T cell proliferation. In the present study, RNA in situ hybridization was used to detect TMEM174 gene expression in various malignant renal cancer and normal renal tissues. The results showed that TMEM174 exhibits differential expression in renal tissues, with a high positive rate of expression in squamous cell carcinoma with necrosis, papillary renal cell carcinoma and transitional cell carcinoma, and a low positive rate of expression in clear cell carcinoma, interstitial nephritis, undifferentiated carcinoma, retroperitoneal metastatic clear cell carcinoma, adrenal gland metastatic clear cell carcinoma, pelvic cavity metastatic chromophobe carcinoma, severe atypical hyperplasia of transitional epithelium and hyperplasia. Extremely weak expression was exhibited in collecting duct carcinoma, Wilms' tumor, chronic pyelonephritis, acute pyelonephritis, cancer adjacent normal renal tissue and normal renal tissue. In conclusion, the TMEM174 gene exhibited high expression levels in certain renal carcinomas, which may indicate that TMEM174 may have a significant role in the development and progression of these renal carcinomas.

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