Knockdown of phosphodiesterase 4D inhibits nasopharyngeal carcinoma proliferation via the epidermal growth factor receptor signaling pathway

  • Authors:
    • Ting Xu
    • Sihai Wu
    • Yuan Yuan
    • Guoxin Yan
    • Dajiang Xiao
  • View Affiliations

  • Published online on: August 8, 2014     https://doi.org/10.3892/ol.2014.2422
  • Pages: 2110-2116
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Abstract

Phosphodiesterase 4D (PDE4D) is a subtype of metallohydrolases, and it has been reported that PDE4D functions as a proliferation promoting factor in certain types of cancer, including head and neck cancer. The present study first investigated the function of PDE4D in nasopharyngeal carcinoma (NPC). Western blot analysis was applied to detect PDE4D expression in NPC samples and cells. A lentiviral infection technique was used to stabilize the knockdown of PDE4D, which was subsequently examined in vitro and in vivo. The results showed that PDE4D was overexpressed in the NPC tissues and cells. Knockdown of PDE4D inhibited the growth of CNE2 and 5‑8F, inducing cell cycle arrest in the G0/G1 phase in CNE2. These effects could be reversed by epidermal growth factor (EGF) stimulation. Furthermore, knockdown of PDE4D significantly inhibited the phosphorylation of epidermal growth factor receptor (EGFR) and AKT. The results were further validated in an NPC xenograft in nude mice. In conclusion, this study demonstrated that PDE4D may function as a proliferation promoting factor in NPC, by affecting the EGFR/PI3K/AKT signaling pathway. Therefore, the targeting of PDE4D may be a rational strategy in the treatment of NPC.
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November-2014
Volume 8 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Xu T, Wu S, Yuan Y, Yan G and Xiao D: Knockdown of phosphodiesterase 4D inhibits nasopharyngeal carcinoma proliferation via the epidermal growth factor receptor signaling pathway. Oncol Lett 8: 2110-2116, 2014
APA
Xu, T., Wu, S., Yuan, Y., Yan, G., & Xiao, D. (2014). Knockdown of phosphodiesterase 4D inhibits nasopharyngeal carcinoma proliferation via the epidermal growth factor receptor signaling pathway. Oncology Letters, 8, 2110-2116. https://doi.org/10.3892/ol.2014.2422
MLA
Xu, T., Wu, S., Yuan, Y., Yan, G., Xiao, D."Knockdown of phosphodiesterase 4D inhibits nasopharyngeal carcinoma proliferation via the epidermal growth factor receptor signaling pathway". Oncology Letters 8.5 (2014): 2110-2116.
Chicago
Xu, T., Wu, S., Yuan, Y., Yan, G., Xiao, D."Knockdown of phosphodiesterase 4D inhibits nasopharyngeal carcinoma proliferation via the epidermal growth factor receptor signaling pathway". Oncology Letters 8, no. 5 (2014): 2110-2116. https://doi.org/10.3892/ol.2014.2422