Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin

Dong,Hanzhang; Liu,Gaogao; Jiang,Biao; Guo,Jiubing; Tao,Guoquan; Yiu,Wei; Zhou,Jingsong; Li,Guoxin

doi:10.3892/ol.2014.2463

Overexpression of the Survivin gene in SGC7901 cell resistance to cisplatin

Authors:
- Hanzhang Dong
- Gaogao Liu
- Biao Jiang
- Jiubing Guo
- Guoquan Tao
- Wei Yiu
- Jingsong Zhou
- Guoxin Li
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Affiliations: Department of General Surgery, The Affiliated Nanfang Hospital of Southern Medical University, Guangzhou, Guangdong 510515, P.R. China, Department of General Surgery, The Affiliated Beijiao Hospital of Southern Medical University, Shunde, Guangdong 528311, P.R. China
Published online on: August 20, 2014 https://doi.org/10.3892/ol.2014.2463
Pages: 1953-1956

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Abstract

The aim of the present study was to investigate the mechanism of SGC7901 cell resistance to cisplatin (CDDP). SGC7901/CDDP cells were established by the long‑term continuous exposure of SGC7901 cells to CDDP in stepwise concentration increments. The morphologies of the SGC7901/CDDP and SGC7901 cells were observed by microscopy. The expression levels of Survivin mRNA and protein in the SGC7901/CDDP and SGC7901 cells were examined by reverse transcription polymerase chain reaction and western blotting respectively. The results revealed morphological differences between the SGC7901 and SGC7901/CDDP cells. The expression levels of Survivin mRNA and protein were significantly higher in the SGC7901/CDDP cells than in the SGC7901 cells. Therefore, high expression levels of the Survivin gene may explain SGC7901 cell resistance to CDDP.

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