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Article

Occurrence of HHIP gene CpG island methylation in gastric cancer

  • Authors:
    • Yu Song
    • Yun Zuo
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Zhangjiagang First People's Hospital, Suzhou, Jiangsu 215600, P.R. China
  • Pages: 2340-2344
    |
    Published online on: September 10, 2014
       https://doi.org/10.3892/ol.2014.2518
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Abstract

The present study aimed to observe the methylation status of the CpG islands at the human hedgehog interacting protein (HHIP) gene in gastric cancer tissues, peritumoral tissues and the AGS cell line, to analyze the association between the methylation status of the CpG islands and the tumorigenesis of gastric cancer. The HHIP mRNA expression in 60 human gastric carcinnoma tissues, peritumoral tissues and the gastric carcinoma AGS cell line were detected by reverse transcription polymerase chain reaction (RT‑PCR). The HHIP methylation status of the promoter region in the gastric carcinnoma tissues and peritumoral tissues was detected by methylation‑specific PCR (MSP). Prior to and following treatment with methyl transferase inhibitor 5‑aza‑2'‑deoxycitydine (5‑aza‑dc), the HHIP mRNA expression level, the methylation status of the promoter region and the methylation site loci on the CpG islands in the AGS cells were detected by RT‑PCR, MSP and bisulfite sequencing PCR (BSP), respectively. The correlation between the methylation status of the CpG islands at the HHIP promoter region and the HHIP mRNA expression level were analyzed. It was found that the expression level of the HHIP mRNA in the gastric carcinoma tissues was significantly lower than that in the adjacent tissues (0.82±0.38 vs. 1.60±0.26, respectively; P<0.001). No significant correlations were observed between the expression of HHIP mRNA and age, gender, tumor‑node‑metastasis stage, differentiation degree and presence of lymph node metastasis (P>0.05). The degree of methylation of the HHIP gene promotor in the peritumoral tissues (17.7±3.59%) was significantly lower than that in the gastric cancer tissues (62.9±6.14%) and in the AGS cells (99.7±0.67%) (P<0.05). Compared with prior to 5‑aza‑dc intervention, the HHIP mRNA expression level in the AGS cells was significantly increased subsequent to intervention (0.21±0.12 vs. 4.68±0.22; P<0.01), while the degree of methylation in the AGS cells was significantly decreased (90.2±0.67 vs. 10.1±0.21%; P<0.01), and the methylation sites in CpG islands were significantly reduced. The degree of HHIP methylation showed a negative correlation with the level of mRNA expression (r=‑0.693; P<0.01). It can be hypothesized that a high degree of methylation of the HHIP gene promoter CpG islands in gastric cancer tissues and cells causes a decrease in HHIP mRNA expression, which may be involved in the carcinogenesis of gastric cancer.
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1 

de Martel C, Forman D and Plummer M: Gastric cancer: epidemiology and risk factors. Gastroenterol Clin North Am. 42:219–240. 2013.

2 

Fock KM: Review article: the epidemiology and prevention of gastric cancer. Aliment Pharmacol There. 40:250–260. 2014.

3 

Bollschweiler E, Berlth F, Baltin C, et al: Treatment of early gastric cancer in the Western World. World J Gastroenterol. 20:5672–5678. 2014.

4 

Sheikh A, Alvi AA, Aslam HM and Haseeb A: Hedgehog pathway inhibitors - current status and future propects. Infect Agent Cancer. 7:292012.

5 

Gerardo Valadez J, Grover VK, Carter MD, et al: Identification of Hedgehog pathway responsive glioblastomas by isocitrate dehydrogenase mutation. Cancer Lett. 328:297–306. 2013.

6 

Yun JI, Kim HR, Park H, et al: Small molecule inhibitors of the hedgehog signaling pathway for the treatment of cancer. Arch Pharm Res. 35:1317–1333. 2012.

7 

Queiroz KC, Spek CA and Peppelenbosch MP: Targeting Hedgehog signaling and understanding refractory response to treatment with Hedgehog pathway inhibitors. Drug Resist Updat. 15:211–222. 2012.

8 

Cobourne MT and Green JB: Hedgehog signaling in development of the secondary palate. Front Oral Biol. 16:52–59. 2012.

9 

Büller NV, Rosekrans SL, Westerlund J, et al: Hedgehog signaling and maintenance of homeostasis in intestinal epithelium. Physiology (Bethesda). 27:148–155. 2012.

10 

Viatte S, Plant D and Raychaudhuri S: Genetics and epigenetics of rheumatoid arthritis. Nat Rev Rheumatol. 9:141–153. 2013.

11 

Eising E, A Datson N, van den Maagdenberg AM and Ferrari MD: Epigenetic mechanisms in migraine: a promising avenue. BMC Med. 11:262013.

12 

Hodes GE: Sex, stress, and epigenetics: regulation of behavior in animal models of mood disorders. Biol Sex Differ. 4:12013.

13 

Toraño EG, Petrus S, Fernandez AF and Fraga MF: Global DNA hypomethylation in cancer: review of validated and clinical significance. Clin Chem Lab Med. 50:1733–1742. 2012.

14 

Savio AJ, Lemire M, Mrkonjic M, et al: MLH1 region polymorphisms show a significant association with CpG island shore methylation in a large cohort of healthy individuals. PLoS One. 7:e515312012.

15 

Kim JG, Takeshima H, Niwa T, et al: Comprehensive DNA methylation and extensive mutation analyses reveal an association between the CpG island methylator phenotype and oncogenic mutations in gastric cancers. Cancer Lett. 330:33–40. 2013.

16 

Anastasiadou C, Malousi A, Maglaveras N and Kouidou S: Human epigenome data reveal increased CpG methylation in alternatively spliced sites and putative exonic splicing enhancers. DNA Cell Biol. 30:267–275. 2011.

17 

Lu JT, Zhao WD, He W and Wei W: Hedgehog signaling pathway mediates invasion and metastasis of hepatocellular carcinoma via ERK pathway. Acta Pharmacol Sin. 33:691–700. 2012.

18 

Yun JI, Kim HR, Park H, et al: Small molecule inhibitors of the hedgehog signaling pathway for the treatment of cancer. Arch Pharm Res. 35:1317–1333. 2012.

19 

Pan JY and Zhou SH: The hedgehog signaling, a new therapeutic target for treatment of ischemic heart disease. Pharmazie. 67:475–481. 2012.

20 

Lee JH, Park SJ, Abraham SC, et al: Frequent CpG island methylation in precursor lesions and early gastric adenocarcinomas. Oncogene. 23:4646–4654. 2004.

21 

Berman A, Mezey M, Kobayashi M, et al: Gerontological nursing content in baccalaureate nursing programs: comparison of findings from 1997 and 2003. J Prof Nurs. 21:268–275. 2005.

22 

Shahi MH, Lorente A and Castresana JS: Hedgehog signalling in medulloblas toma, glioblastoma and neuroblastoma. Oncol Rep. 19:681–688. 2008.

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Copy and paste a formatted citation
Spandidos Publications style
Song Y and Zuo Y: Occurrence of HHIP gene CpG island methylation in gastric cancer. Oncol Lett 8: 2340-2344, 2014.
APA
Song, Y., & Zuo, Y. (2014). Occurrence of HHIP gene CpG island methylation in gastric cancer. Oncology Letters, 8, 2340-2344. https://doi.org/10.3892/ol.2014.2518
MLA
Song, Y., Zuo, Y."Occurrence of HHIP gene CpG island methylation in gastric cancer". Oncology Letters 8.5 (2014): 2340-2344.
Chicago
Song, Y., Zuo, Y."Occurrence of HHIP gene CpG island methylation in gastric cancer". Oncology Letters 8, no. 5 (2014): 2340-2344. https://doi.org/10.3892/ol.2014.2518
Copy and paste a formatted citation
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Spandidos Publications style
Song Y and Zuo Y: Occurrence of HHIP gene CpG island methylation in gastric cancer. Oncol Lett 8: 2340-2344, 2014.
APA
Song, Y., & Zuo, Y. (2014). Occurrence of HHIP gene CpG island methylation in gastric cancer. Oncology Letters, 8, 2340-2344. https://doi.org/10.3892/ol.2014.2518
MLA
Song, Y., Zuo, Y."Occurrence of HHIP gene CpG island methylation in gastric cancer". Oncology Letters 8.5 (2014): 2340-2344.
Chicago
Song, Y., Zuo, Y."Occurrence of HHIP gene CpG island methylation in gastric cancer". Oncology Letters 8, no. 5 (2014): 2340-2344. https://doi.org/10.3892/ol.2014.2518
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