Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study

Ferreira Martinez,Elizabeth; Dias Demasi,Ana  Paula; Napimoga,Marcelo  Henrique; Barcellos Silva,Carolina  Amália; Festugatto Navarini,Natalia; Soares Araújo,Ney; Cavalcanti de Araújo,Vera

doi:10.3892/ol.2014.2624

Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study

Authors:
- Elizabeth Ferreira Martinez
- Ana Paula Dias Demasi
- Marcelo Henrique Napimoga
- Carolina Amália Barcellos Silva
- Natalia Festugatto Navarini
- Ney Soares Araújo
- Vera Cavalcanti de Araújo
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Affiliations: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-610, Brazil
Published online on: October 17, 2014 https://doi.org/10.3892/ol.2014.2624
Pages: 313-317

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Abstract

Myoepithelial cells have been implicated in the regulation of the transition from in situ to invasive neoplasia in salivary gland tumors. Considering the importance of the microenvironment of the tumor, the present in vitro study therefore analyzed the morphological and phenotypic changes undergone by benign myoepithelial cells from pleomorphic adenoma (PA) stimulated by tumor‑conditioned medium. The benign myoepithelial cells were obtained from PA and were cultured with fibronectin extracellular matrix protein, supplemented with tumor‑conditioned medium, which was harvested from breast ductal adenocarcinoma AU‑565 and melanoma Hs 852.T cells. The morphological alterations were assessed by immunofluorescence analysis using vimentin antibody. The α‑smooth muscle actin (α‑SMA) and fibroblast growth factor (FGF)‑2 proteins were analyzed by indirect immunofluorescence and quantitative polymerase chain reaction (qPCR). No morphological changes were observed in the myoepithelial cells cultured in fibronectin protein under stimulation from either tumor‑conditioned medium. The immunofluorescence results, which were supported by qPCR analysis, revealed that only α‑SMA was upregulated in the fibronectin substratum, with or without tumor‑conditioned medium obtained from breast ductal adenocarcinoma and melanoma cells. No significant difference in FGF‑2 mRNA expression was detected when the cells were cultured either in the tumor‑conditioned medium or in the fibronectin substratum. The tumor‑conditioned medium harvested from breast ductal adenocarcinoma and melanoma did not affect myoepithelial cell differentiation and function, which was reflected by the fact that there was no observed increase in α‑SMA and FGF‑2 expression, respectively.

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