Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
January-2015 Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study

  • Authors:
    • Elizabeth Ferreira Martinez
    • Ana Paula Dias Demasi
    • Marcelo Henrique Napimoga
    • Carolina Amália Barcellos Silva
    • Natalia Festugatto Navarini
    • Ney Soares Araújo
    • Vera Cavalcanti de Araújo
  • View Affiliations / Copyright

    Affiliations: Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Campinas, São Paulo 13045-610, Brazil
  • Pages: 313-317
    |
    Published online on: October 17, 2014
       https://doi.org/10.3892/ol.2014.2624
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Myoepithelial cells have been implicated in the regulation of the transition from in situ to invasive neoplasia in salivary gland tumors. Considering the importance of the microenvironment of the tumor, the present in vitro study therefore analyzed the morphological and phenotypic changes undergone by benign myoepithelial cells from pleomorphic adenoma (PA) stimulated by tumor‑conditioned medium. The benign myoepithelial cells were obtained from PA and were cultured with fibronectin extracellular matrix protein, supplemented with tumor‑conditioned medium, which was harvested from breast ductal adenocarcinoma AU‑565 and melanoma Hs 852.T cells. The morphological alterations were assessed by immunofluorescence analysis using vimentin antibody. The α‑smooth muscle actin (α‑SMA) and fibroblast growth factor (FGF)‑2 proteins were analyzed by indirect immunofluorescence and quantitative polymerase chain reaction (qPCR). No morphological changes were observed in the myoepithelial cells cultured in fibronectin protein under stimulation from either tumor‑conditioned medium. The immunofluorescence results, which were supported by qPCR analysis, revealed that only α‑SMA was upregulated in the fibronectin substratum, with or without tumor‑conditioned medium obtained from breast ductal adenocarcinoma and melanoma cells. No significant difference in FGF‑2 mRNA expression was detected when the cells were cultured either in the tumor‑conditioned medium or in the fibronectin substratum. The tumor‑conditioned medium harvested from breast ductal adenocarcinoma and melanoma did not affect myoepithelial cell differentiation and function, which was reflected by the fact that there was no observed increase in α‑SMA and FGF‑2 expression, respectively.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Barsky SH and Karlin NJ: Mechanisms of disease: breast tumor pathogenesis and the role of the myoepithelial cell. Nat Clin Pract Oncol. 3:138–151. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Nguyen M, Lee MC, Wang JL, Tomlinson JS, Shao ZM, Alpaugh ML and Barsky SH: The human myoepithelial cell displays a multifaceted anti-angiogenic phenotype. Oncogene. 19:3449–3459. 2000. View Article : Google Scholar : PubMed/NCBI

3 

Deugnier MA, Teulière J, Faraldo MM, Thiery JP and Glukhova MA: The importance of being a myoepithelial cell. Breast Cancer Res. 4:224–230. 2002. View Article : Google Scholar : PubMed/NCBI

4 

Polyak K and Hu M: Do myoepithelial cells hold the key for breast tumor progression? J Mammary Gland Biol Neoplasia. 10:231–247. 2005. View Article : Google Scholar

5 

da Silva A, Silva CA, Montalli VA, Martinez EF, de Araújo VC and Furuse C: In vitro evaluation of the suppressor potential of conditioned medium from benign myoepithelial cells from pleomorphic adenoma in malignant cell invasion. J Oral Pathol Med. 41:610–614. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Bissell MJ, Kenny PA and Radisky DC: Microenvironmental regulators of tissue structure and function also regulate tumor induction and progression: the role of extracellular matrix and its degrading enzymes. Cold Spring Harb Symp Quant Biol. 70:343–356. 2005. View Article : Google Scholar

7 

de Araújo VC, Altemani A, Furuse C, Martins MT and de Araújo NS: Immunoprofile of reatctive salivary myoepithelial cells in intraductal areas of carcinoma ex-pleomorphic adenoma. Oral Oncol. 42:1011–1016. 2006. View Article : Google Scholar

8 

Martinez EF, Demasi AP, Miguita L, Altemani A, de Araújo NS and de Araújo VC: FGF-2 is overexpressed in myoepithelial cells of carcinoma ex-pleomorphic adenoma in situ structures. Oncol Rep. 24:155–160. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Martinez EF, Demasi AP, Napimoga MH, Arana-Chavez VE, Altemani A, de Araújo NS and de Araújo VC: In vitro influence of the extracellular matrix in myoepithelial cells stimulated by malignant conditioned medium. Oral Oncol. 48:102–109. 2012. View Article : Google Scholar

10 

Martinez EF, Montaldi PT, de Araújo NS, Altemani A and de Araújo VC: A proposal of an in vitro model which mimics in situ areas of carcinoma. J Cell Comun Signal. 6:107–109. 2012. View Article : Google Scholar

11 

Canel M, Serrels A, Frame MC and Brunton VG: E-cadherin-integrin crosstalk in cancer invasion and metastasis. J Cell Sci. 126:393–401. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Altemani A, Martins MT, Freitas L, Soares F, Araújo NS and Araújo VC: Carcinoma ex pleomorphic adenoma (CXPA): immunoprofile of the cells involved in carcinomatous progression. Histophatol. 46:635–641. 2005. View Article : Google Scholar

13 

Pia-Foschini M, Reis-Filho JS, Eusebi V and Lakhani SR: Salivary gland-like tumors of the breast: surgical and molecular pathology. J Clin Pathol. 56:497–506. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Gudjonsson T, Adriance MC, Sternlicht MD, Petersen OW and Bissel MJ: Myoepithelial cells: their origin and function in breast morphogenesis and neoplasia. J Mammary Gland Biol Neoplasia. 10:261–272. 2005. View Article : Google Scholar

15 

Kang KH, Ling TY, Liou HH, Huang YK, Hour MJ, Liou HC and Fu WM: Enhancement role of host 12/15-lipoxygenase in melanoma progression. Eur J Cancer. 49:2747–2759. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Araújo VC, Furuse C, Cury PR, Altemani A, Alves VA and de Araújo NS: Tenascin and fibronectin expression in carcinoma ex pleomorphic adenoma. Appl Immunohistochem Mol Morphol. 16:48–53. 2008.

17 

Araújo VC, Demasi AP, Furuse C, Altemani A, Alves VA, Freitas LL and Araújo NS: Collagen type I may influence the expression of E-cadherin and beta-catenin in carcinoma ex-pleomorphic adenoma. Appl Immunohistochem Mol Morphol. 17:312–318. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Park J and Schwarzbauer JE: Mammary epithelial cell interactions with fibronectin stimulate epithelial-mesenchymal transition. Oncogene. 27:1649–1657. 2014. View Article : Google Scholar

19 

Gehler S, Ponik SM, Riching KM and Keely PJ: Bi-directional signaling: extracellular matrix and integrin regulation of breast tumor progression. Crit Rev Eukaryot Gene Expr. 23:139–157. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Whiteside TL: The role of death receptor ligands in shaping tumor microenvironment. Immunol Invest. 36:25–46. 2007. View Article : Google Scholar

21 

Bianchi G, Borgonovo G, Pistoia V and Raffaghello L: Immunosuppressive cells and tumour microenvironment: focus on mesenchymal stem cells and myeloid derived suppressor cells. Histol Histopathol. 26:941–951. 2011.PubMed/NCBI

22 

Martinez EF, Napimoga MH, Montalli VA, de Araújo NS and de Araújo VC: In vitro cytokine expression in in situ-like areas of malignant neoplasia. Arch Oral Biol. 58:552–557. 2013. View Article : Google Scholar

23 

Sternlicht MD, Kedeshian P, Shao ZM, Safarians S and Barsky SH: The human myoepithelial cell is a natural tumor suppressor. Clin Cancer Res. 3:1949–1958. 1997.

24 

Sternlicht MD and Barsky SH: The myoepithelial defense: a host defense against cancer. Med Hypotheses. 48:37–46. 1997. View Article : Google Scholar : PubMed/NCBI

25 

Barsky SH and Alpaugh ML: Myoepithelium: methods of culture and study. Culture of Human Tumor Cells. Freshney RI: Wiley-Liss; New Jersey: pp. 221–261. 2004

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ferreira Martinez E, Dias Demasi AP, Napimoga MH, Barcellos Silva CA, Festugatto Navarini N, Soares Araújo N and Cavalcanti de Araújo V: Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study. Oncol Lett 9: 313-317, 2015.
APA
Ferreira Martinez, E., Dias Demasi, A.P., Napimoga, M.H., Barcellos Silva, C.A., Festugatto Navarini, N., Soares Araújo, N., & Cavalcanti de Araújo, V. (2015). Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study. Oncology Letters, 9, 313-317. https://doi.org/10.3892/ol.2014.2624
MLA
Ferreira Martinez, E., Dias Demasi, A. P., Napimoga, M. H., Barcellos Silva, C. A., Festugatto Navarini, N., Soares Araújo, N., Cavalcanti de Araújo, V."Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study". Oncology Letters 9.1 (2015): 313-317.
Chicago
Ferreira Martinez, E., Dias Demasi, A. P., Napimoga, M. H., Barcellos Silva, C. A., Festugatto Navarini, N., Soares Araújo, N., Cavalcanti de Araújo, V."Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study". Oncology Letters 9, no. 1 (2015): 313-317. https://doi.org/10.3892/ol.2014.2624
Copy and paste a formatted citation
x
Spandidos Publications style
Ferreira Martinez E, Dias Demasi AP, Napimoga MH, Barcellos Silva CA, Festugatto Navarini N, Soares Araújo N and Cavalcanti de Araújo V: Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study. Oncol Lett 9: 313-317, 2015.
APA
Ferreira Martinez, E., Dias Demasi, A.P., Napimoga, M.H., Barcellos Silva, C.A., Festugatto Navarini, N., Soares Araújo, N., & Cavalcanti de Araújo, V. (2015). Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study. Oncology Letters, 9, 313-317. https://doi.org/10.3892/ol.2014.2624
MLA
Ferreira Martinez, E., Dias Demasi, A. P., Napimoga, M. H., Barcellos Silva, C. A., Festugatto Navarini, N., Soares Araújo, N., Cavalcanti de Araújo, V."Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study". Oncology Letters 9.1 (2015): 313-317.
Chicago
Ferreira Martinez, E., Dias Demasi, A. P., Napimoga, M. H., Barcellos Silva, C. A., Festugatto Navarini, N., Soares Araújo, N., Cavalcanti de Araújo, V."Myoepithelial cells from pleomorphic adenoma are not influenced by tumor conditioned media from breast ductal denocarcinoma and melanoma cells: An in vitro study". Oncology Letters 9, no. 1 (2015): 313-317. https://doi.org/10.3892/ol.2014.2624
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team