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Article

Long‑fragment DNA as a potential marker for stool‑based detection of colorectal cancer

  • Authors:
    • Yibo Zhang
    • Yutaka Suehiro
    • Yoshitaro Shindo
    • Kouhei Sakai
    • Shoichi Hazama
    • Shingo Higaki
    • Isao Sakaida
    • Masaaki Oka
    • Takahiro Yamasaki
  • View Affiliations / Copyright

    Affiliations: Department of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755‑8505, Japan, Department of Digestive Surgery and Surgical Oncology (Surgery II), Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755‑8505, Japan, Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi 755‑8505, Japan
  • Pages: 454-458
    |
    Published online on: October 24, 2014
       https://doi.org/10.3892/ol.2014.2632
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Abstract

Neoplastic cells that are exfoliated from the colorectal epithelium exhibit dysfunctional apoptotic mechanisms, and thus it is possible to identify high‑molecular‑weight DNA fragments (long DNA) in feces. In the present study, the sensitivity and specificity of fecal‑based long DNA assays were evaluated for the detection of colorectal cancer (CRC). Feces were collected from 54 healthy volunteers and 130 patients with CRC prior to surgical treatment. The presence of long DNA of the adenomatosis polyposis coli, Kirsten rat sarcoma viral oncogene homolog (KRAS), B‑raf proto‑oncogene, serine/threonine kinase and p53 genes was assessed by polymerase chain reaction followed by electrophoresis. The identification of long DNA in feces was found to exhibit a sensitivity of 56.2% and specificity of 96.3% for CRC detection. In addition, long DNA was identified in the feces of 58/90 (64.4%) patients with distal CRC and 15/40 (37.5%) patients with proximal CRC. This study indicates the potential of the fecal long DNA assay as a non‑invasive and easily performed method for the detection of individuals with CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Suehiro Y, Shindo Y, Sakai K, Hazama S, Higaki S, Sakaida I, Oka M and Yamasaki T: Long‑fragment DNA as a potential marker for stool‑based detection of colorectal cancer. Oncol Lett 9: 454-458, 2015.
APA
Zhang, Y., Suehiro, Y., Shindo, Y., Sakai, K., Hazama, S., Higaki, S. ... Yamasaki, T. (2015). Long‑fragment DNA as a potential marker for stool‑based detection of colorectal cancer. Oncology Letters, 9, 454-458. https://doi.org/10.3892/ol.2014.2632
MLA
Zhang, Y., Suehiro, Y., Shindo, Y., Sakai, K., Hazama, S., Higaki, S., Sakaida, I., Oka, M., Yamasaki, T."Long‑fragment DNA as a potential marker for stool‑based detection of colorectal cancer". Oncology Letters 9.1 (2015): 454-458.
Chicago
Zhang, Y., Suehiro, Y., Shindo, Y., Sakai, K., Hazama, S., Higaki, S., Sakaida, I., Oka, M., Yamasaki, T."Long‑fragment DNA as a potential marker for stool‑based detection of colorectal cancer". Oncology Letters 9, no. 1 (2015): 454-458. https://doi.org/10.3892/ol.2014.2632
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Suehiro Y, Shindo Y, Sakai K, Hazama S, Higaki S, Sakaida I, Oka M and Yamasaki T: Long‑fragment DNA as a potential marker for stool‑based detection of colorectal cancer. Oncol Lett 9: 454-458, 2015.
APA
Zhang, Y., Suehiro, Y., Shindo, Y., Sakai, K., Hazama, S., Higaki, S. ... Yamasaki, T. (2015). Long‑fragment DNA as a potential marker for stool‑based detection of colorectal cancer. Oncology Letters, 9, 454-458. https://doi.org/10.3892/ol.2014.2632
MLA
Zhang, Y., Suehiro, Y., Shindo, Y., Sakai, K., Hazama, S., Higaki, S., Sakaida, I., Oka, M., Yamasaki, T."Long‑fragment DNA as a potential marker for stool‑based detection of colorectal cancer". Oncology Letters 9.1 (2015): 454-458.
Chicago
Zhang, Y., Suehiro, Y., Shindo, Y., Sakai, K., Hazama, S., Higaki, S., Sakaida, I., Oka, M., Yamasaki, T."Long‑fragment DNA as a potential marker for stool‑based detection of colorectal cancer". Oncology Letters 9, no. 1 (2015): 454-458. https://doi.org/10.3892/ol.2014.2632
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