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Isolation, cultivation and identification of human lung adenocarcinoma stem cells

  • Authors:
    • De‑Geng Zhang
    • Ai‑Gui Jiang
    • Hui‑Yu Lu
    • Li‑Xin Zhang
    • Xiao‑Yan Gao
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China, Department of Respiratory Medicine, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China, Institute of Clinical Medicine, Taizhou People's Hospital Affiliated to Medical College of Nantong University, Taizhou, Jiangsu 225300, P.R. China
  • Pages: 47-54
    |
    Published online on: October 24, 2014
       https://doi.org/10.3892/ol.2014.2639
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Abstract

Recently, an increasing number of studies have demonstrated that lung cancer is a stem cell disease. However, ideal cell surface markers for isolating stem cells in lung cancer are yet to be identified. In the present study, a cell population with a cluster of differentiation (CD)133+ phenotype was successfully isolated from a single cell suspension of lung adenocarcinoma tissue using magnetic‑activated cell sorting (MACS) and enriched in a serum‑free culture. In comparison to CD133‑ cells, the CD133+ cells exhibited an enhanced capacity for self‑renewal and differentiation, and a greater potential for in vivo tumor formation, in non‑obese diabetic/severe combined immunodeficient (NOD/SCID) mice. Tumors could be induced in NOD/SCID mice by the transplantation of 102 stem‑like cells per mouse. The results of the present study demonstrated that CD133 may serve as a specific cell surface marker for lung adenocarcinoma stem cells, and that MACS combined with serum‑free culture is an effective method for isolating and enriching lung cancer stem cells.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang DG, Jiang AG, Lu HY, Zhang LX and Gao XY: Isolation, cultivation and identification of human lung adenocarcinoma stem cells. Oncol Lett 9: 47-54, 2015.
APA
Zhang, D., Jiang, A., Lu, H., Zhang, L., & Gao, X. (2015). Isolation, cultivation and identification of human lung adenocarcinoma stem cells. Oncology Letters, 9, 47-54. https://doi.org/10.3892/ol.2014.2639
MLA
Zhang, D., Jiang, A., Lu, H., Zhang, L., Gao, X."Isolation, cultivation and identification of human lung adenocarcinoma stem cells". Oncology Letters 9.1 (2015): 47-54.
Chicago
Zhang, D., Jiang, A., Lu, H., Zhang, L., Gao, X."Isolation, cultivation and identification of human lung adenocarcinoma stem cells". Oncology Letters 9, no. 1 (2015): 47-54. https://doi.org/10.3892/ol.2014.2639
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang DG, Jiang AG, Lu HY, Zhang LX and Gao XY: Isolation, cultivation and identification of human lung adenocarcinoma stem cells. Oncol Lett 9: 47-54, 2015.
APA
Zhang, D., Jiang, A., Lu, H., Zhang, L., & Gao, X. (2015). Isolation, cultivation and identification of human lung adenocarcinoma stem cells. Oncology Letters, 9, 47-54. https://doi.org/10.3892/ol.2014.2639
MLA
Zhang, D., Jiang, A., Lu, H., Zhang, L., Gao, X."Isolation, cultivation and identification of human lung adenocarcinoma stem cells". Oncology Letters 9.1 (2015): 47-54.
Chicago
Zhang, D., Jiang, A., Lu, H., Zhang, L., Gao, X."Isolation, cultivation and identification of human lung adenocarcinoma stem cells". Oncology Letters 9, no. 1 (2015): 47-54. https://doi.org/10.3892/ol.2014.2639
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