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Article

Increased BRAF copy number in lung adenocarcinoma

  • Authors:
    • Hidefumi Sasaki
    • Masahiko Maekawa
    • Tsutomu Tatematsu
    • Katsuhiro  Okuda
    • Satoru Moriyama
    • Motoki Yano
    • Yoshitaka Fujii
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, Immunology and Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Aichi 467‑8601, Japan, GSP Lab Inc., Kawasaki, Kanagawa 212‑0032, Japan
  • Pages: 709-712
    |
    Published online on: November 20, 2014
       https://doi.org/10.3892/ol.2014.2719
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Abstract

Point mutation of the BRAF gene is a genetic event that occurs in a subset of lung adenocarcinoma cases. For example, BRAF V600E is a driver mutation that can be effectively targeted using selective BRAF and/or MEK inhibitors. The present study hypothesized that an increase in BRAF copy number may be correlated with certain clinicopathological features of lung adenocarcinoma in Japanese patients. The BRAF gene copy number was analyzed using quantitative polymerase chain reaction amplifications in 29 surgically treated lung adenocarcinoma cases without EGFR or Kras mutations from Nagoya City University Hospital (Nagoya, Japan). Seven BRAF‑mutant cases were included. Increased BRAF gene copy number was identified in three lung adenocarcinoma patients (10.3%), all of which exhibited the V600E mutation. Using fluorescence in situ hybridization with BRAF‑specific and chromosome 7 centromeric probes, increased copy number status was associated with gene amplification or gain of chromosome 7. Although increased BRAF copy number was correlated with BRAF V600E mutations, numerical changes in BRAF copy number were rare and mild in lung adenocarcinoma, resulting in no significant difference in pathological tumor status or tumor stage.
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Copy and paste a formatted citation
Spandidos Publications style
Sasaki H, Maekawa M, Tatematsu T, Okuda K, Moriyama S, Yano M and Fujii Y: Increased BRAF copy number in lung adenocarcinoma. Oncol Lett 9: 709-712, 2015.
APA
Sasaki, H., Maekawa, M., Tatematsu, T., Okuda, K., Moriyama, S., Yano, M., & Fujii, Y. (2015). Increased BRAF copy number in lung adenocarcinoma. Oncology Letters, 9, 709-712. https://doi.org/10.3892/ol.2014.2719
MLA
Sasaki, H., Maekawa, M., Tatematsu, T., Okuda, K., Moriyama, S., Yano, M., Fujii, Y."Increased BRAF copy number in lung adenocarcinoma". Oncology Letters 9.2 (2015): 709-712.
Chicago
Sasaki, H., Maekawa, M., Tatematsu, T., Okuda, K., Moriyama, S., Yano, M., Fujii, Y."Increased BRAF copy number in lung adenocarcinoma". Oncology Letters 9, no. 2 (2015): 709-712. https://doi.org/10.3892/ol.2014.2719
Copy and paste a formatted citation
x
Spandidos Publications style
Sasaki H, Maekawa M, Tatematsu T, Okuda K, Moriyama S, Yano M and Fujii Y: Increased BRAF copy number in lung adenocarcinoma. Oncol Lett 9: 709-712, 2015.
APA
Sasaki, H., Maekawa, M., Tatematsu, T., Okuda, K., Moriyama, S., Yano, M., & Fujii, Y. (2015). Increased BRAF copy number in lung adenocarcinoma. Oncology Letters, 9, 709-712. https://doi.org/10.3892/ol.2014.2719
MLA
Sasaki, H., Maekawa, M., Tatematsu, T., Okuda, K., Moriyama, S., Yano, M., Fujii, Y."Increased BRAF copy number in lung adenocarcinoma". Oncology Letters 9.2 (2015): 709-712.
Chicago
Sasaki, H., Maekawa, M., Tatematsu, T., Okuda, K., Moriyama, S., Yano, M., Fujii, Y."Increased BRAF copy number in lung adenocarcinoma". Oncology Letters 9, no. 2 (2015): 709-712. https://doi.org/10.3892/ol.2014.2719
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