NPY1R is a novel peripheral blood marker predictive of metastasis and prognosis in breast cancer patients

Liu,Lei; Xu,Qian; Cheng,Luyang; Ma,Chunhu; Xiao,Lijun; Xu,Dawei; Gao,Yaxian; Wang,Jianping; Song,Hongru

doi:10.3892/ol.2014.2721

February-2015 Volume 9 Issue 2

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

February-2015 Volume 9 Issue 2

Full Size Image

Article

NPY1R is a novel peripheral blood marker predictive of metastasis and prognosis in breast cancer patients

Authors:
- Lei Liu
- Qian Xu
- Luyang Cheng
- Chunhu Ma
- Lijun Xiao
- Dawei Xu
- Yaxian Gao
- Jianping Wang
- Hongru Song
View Affiliations / Copyright

Affiliations: Department of Immunology, Basic Medical Institute, Chengde Medical College, Chengde, Hebei 067000, P.R. China, Department of Central Laboratory, Basic Medical Institute, Chengde Medical College, Chengde, Hebei 067000, P.R. China, Clinical Skills Center, Chengde Medical College, Chengde, Hebei 067000, P.R. China
Pages: 891-896
|
Published online on: November 20, 2014

https://doi.org/10.3892/ol.2014.2721
Expand metrics +

Abstract

The aim of the current study was to evaluate a novel tumor marker, neuropeptide Y receptor Y1 (NPY1R), for the detection of circulating cancer cells and to investigate its clinical significance in breast cancer patients. The Digital Gene Expression Displayer tool of the Cancer Genome Anatomy Project was used to identify the marker gene NPY1R, which is able to detect circulating cancer cells. Nested quantitative polymerase chain reaction was performed to correlate the NPY1R expression levels with the clinicopathological features of 142 breast cancer patients. A follow‑up study of 131 of the breast cancer patients was conducted for 38 months. Compared with the 60 normal control individuals, NPY1R was highly expressed in the cancer patients (P<0.01). These high levels of NPY1R expression were positively correlated with the clinical stage and lymph node metastasis status of the disease, as well as with the status of the estrogen and progesterone receptors (P<0.05). Breast cancer patients with circulating cancer cells that expressed NPY1R exhibited shorter tumor‑specific survival when compared with those with no NPY1R expression (P<0.01). Additionally, the mortality rate was associated with HER2 expression in the NPY1R positive and negative groups. These results indicate that NPY1R may serve as a useful marker to predict breast cancer metastasis and to evaluate the prognosis of breast cancer patients.

View Figures

View References

1	Suh MA, Atashili J, Fuh EA and Eta VA: Breast self-examination and breast cancer awareness in women in developing countries: a survey of women in Buea, Cameroon. BMC Res Notes. 5:6272012. View Article : Google Scholar : PubMed/NCBI
2	Yilmaz YE, Lawless JF, Andrulis IL and Bull SB: Insights from mixture cure modeling of molecular markers for prognosis in breast cancer. J Clin Oncol. 31:2047–2054. 2013. View Article : Google Scholar : PubMed/NCBI
3	Park Y, Chang M, Lee S, et al: Heterogeneity of triple negative breast cancer (TNBC): TNBC might be divided into two or more subgroups by clinicopathologic findings. Cancer Res. 69:(Abstract 6032; Suppl 24). 2009. View Article : Google Scholar
4	Kohno D and Yada T: Arcuate NPY neurons sense and integrate peripheral metabolic signals to control feeding. Neuropeptides. 46:315–319. 2012. View Article : Google Scholar : PubMed/NCBI
5	Memminger M, Keller M, Lopuch M, et al: The neuropeptide y y(1) receptor: a diagnostic marker? Expression in mcf-7 breast cancer cells is down-regulated by antiestrogens in vitro and in xenografts. PLoS One. 7:e510322012. View Article : Google Scholar : PubMed/NCBI
6	Edge SB and Compton CC: The American Joint Committee on Cancer: the 7th edition of the AJCC cancer staging manual and the future of TNM. Ann Surg Oncol. 17:1471–1474. 2010. View Article : Google Scholar : PubMed/NCBI
7	Wolff AC, Hammond ME, Schwartz JN, et al: American Society of Clinical Oncology/College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer. J Clin Oncol. 25:118–145. 2007. View Article : Google Scholar
8	Rahbari NN, Bork U, Motschall E, et al: Molecular detection of tumor cells in regional lymph nodes is associated with disease recurrence and poor survival in node-negative colorectal cancer: a systematic review and meta-analysis. J Clin Oncol. 30:60–70. 2012. View Article : Google Scholar
9	Liu L, Liao GQ, He P, Zhu H, et al: Detection of circulating cancer cells in lung cancer patients with a panel of marker genes. Biochem Biophys Res Commun. 372:756–760. 2008. View Article : Google Scholar : PubMed/NCBI
10	Kavak E, Ünlü M, Nistér M and Koman A: Meta-analysis of cancer gene expression signatures reveals new cancer genes, SAGE tags and tumor associated regions of co-regulation. Nucleic Acids Res. 38:7008–7021. 2010. View Article : Google Scholar : PubMed/NCBI
11	Blackhall F, Peters S, Kerr KM, et al: Biomarkers. Ann Onc. 23:ix73–ix94. 2012. View Article : Google Scholar
12	Higuchi H: Molecular analysis of central feeding regulation by neuropeptide Y (NPY) neurons with NPY receptor small interfering RNAs (siRNAs). Neurochem Int. 61:936–941. 2012. View Article : Google Scholar : PubMed/NCBI
13	Kiyokawa M, Matsuzaki T, Iwasa T, et al: Neuropeptide Y mediates orexin A-mediated suppression of pulsatile gonadotropin-releasing hormone secretion in ovariectomized rats. J Med Invest. 58:11–18. 2011. View Article : Google Scholar : PubMed/NCBI
14	Körner M, Waser B and Reubi JC: High expression of neuropeptide Y1 receptors in ewing sarcoma tumors. Clin Cancer Res. 14:5043–5049. 2008. View Article : Google Scholar : PubMed/NCBI
15	Lu C, Tilan JU, Everhart L, et al: Dipeptidyl peptidases as survival factors in Ewing sarcoma family of tumors: implications for tumor biology and therapy. J Biol Chem. 286:27494–27505. 2011. View Article : Google Scholar : PubMed/NCBI
16	Ruscica M, Dozio E, Boghossian S, et al: Activation of the Y1 receptor by neuropeptide Y regulates the growth of prostate cancer cells. Endocrinology. 147:1466–1473. 2006. View Article : Google Scholar
17	Körner M, Waser B and Reubi JC: High expression of neuropeptide y receptors in tumors of the human adrenal gland and extra-adrenal paraganglia. Clin Cancer Res. 10:8426–8433. 2004. View Article : Google Scholar : PubMed/NCBI
18	Reubi JC, Gugger M, Waser B and Schaer JC: Y(1)-mediated effect of neuropeptide Y in cancer: breast carcinomas as targets. Cancer Res. 61:4636–4641. 2001.PubMed/NCBI
19	Amlal H, Faroqui S, Balasubramaniam A and Sheriff S: Estrogen up-regulates neuropeptide Y Y1 receptor expression in a human breast cancer cell line. Cancer Res. 66:3706–3714. 2006. View Article : Google Scholar : PubMed/NCBI
20	Sheriff S, Ali M, Yahya A, et al: Neuropeptide Y Y5 receptor promotes cell growth through extracellular signal-regulated kinase signaling and cyclic AMP inhibition in a human breast cancer cell line. Mol Cancer Res. 8:604–614. 2010. View Article : Google Scholar : PubMed/NCBI
21	Lennon S, Barton C, Banken L, et al: Utility of serum HER2 extracellular domain assessment in clinical decision making: pooled analysis of four trials of trastuzumab in metastatic breast cancer. J Clin Oncol. 27:1685–1693. 2009. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

NPY1R is a novel peripheral blood marker predictive of metastasis and prognosis in breast cancer patients

This article is mentioned in:

Abstract

Figure 1

Figure 2

Related Articles