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Article

Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab

  • Authors:
    • Aniello De Rosa
    • Silvia Zappavigna
    • Maria Rosaria Villa
    • Salvatore  Improta
    • Elena Cesario
    • Lucia  Mastrullo
    • Michele  Caraglia
    • Paola Stiuso
  • View Affiliations / Copyright

    Affiliations: Local Sanitary Agency, Naples 3 Southern, Torre del Grecco, Naples I‑80059, Italy, Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Naples I‑80138, Italy, Hematology Unit, San Gennaro Hospital, Naples I‑80131, Italy
  • Pages: 1327-1332
    |
    Published online on: December 19, 2014
       https://doi.org/10.3892/ol.2014.2817
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Abstract

Principally located in the outer mitochondrial membrane, the translocator protein (TSPO) is an 18‑kDa transmembrane protein that is a key component of the mitochondrial permeability transition pore. TSPO is associated with a number of biological processes, including apoptosis, the regulation of cellular proliferation, porphyrin transport and heme biosynthesis, immunomodulation, anion transport and the regulation of steroidogenesis. Thus, numerous studies have proposed TSPO as a promising target for novel therapeutic agents, particularly for the treatment of cancer. In the present study, the response of 30 consecutive chronic lymphocytic leukemia (CLL) patients to bendamustine and rituximab treatment was evaluated according to TSPO expression levels. Furthermore, thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) levels, as well as caspase‑3 activity were determined. Compared with the lymphocytes of healthy donors, the 30 consecutive CLL patients exhibited increased TSPO expression levels, decreased TBARS and NO levels and reduced caspase‑3 activity. Six months after the treatment commenced, the TSPO/mitochondria ratio resembled that of the healthy controls in 24/30 CLL patients. In addition, an increase in TBARS and NO levels, two markers of oxidative stress, and a potentiation of caspase‑3 activity in all responder patients was observed. Notably, the six patients who appeared to be resistant to treatment also displayed higher TSPO levels, and lower caspase‑3 activity and TBARS levels. These data indicate that TSPO expression may be a molecular prognostic factor in CLL patients.
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Copy and paste a formatted citation
Spandidos Publications style
De Rosa A, Zappavigna S, Villa MR, Improta S, Cesario E, Mastrullo L, Caraglia M and Stiuso P: Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab. Oncol Lett 9: 1327-1332, 2015.
APA
De Rosa, A., Zappavigna, S., Villa, M.R., Improta, S., Cesario, E., Mastrullo, L. ... Stiuso, P. (2015). Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab. Oncology Letters, 9, 1327-1332. https://doi.org/10.3892/ol.2014.2817
MLA
De Rosa, A., Zappavigna, S., Villa, M. R., Improta, S., Cesario, E., Mastrullo, L., Caraglia, M., Stiuso, P."Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab". Oncology Letters 9.3 (2015): 1327-1332.
Chicago
De Rosa, A., Zappavigna, S., Villa, M. R., Improta, S., Cesario, E., Mastrullo, L., Caraglia, M., Stiuso, P."Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab". Oncology Letters 9, no. 3 (2015): 1327-1332. https://doi.org/10.3892/ol.2014.2817
Copy and paste a formatted citation
x
Spandidos Publications style
De Rosa A, Zappavigna S, Villa MR, Improta S, Cesario E, Mastrullo L, Caraglia M and Stiuso P: Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab. Oncol Lett 9: 1327-1332, 2015.
APA
De Rosa, A., Zappavigna, S., Villa, M.R., Improta, S., Cesario, E., Mastrullo, L. ... Stiuso, P. (2015). Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab. Oncology Letters, 9, 1327-1332. https://doi.org/10.3892/ol.2014.2817
MLA
De Rosa, A., Zappavigna, S., Villa, M. R., Improta, S., Cesario, E., Mastrullo, L., Caraglia, M., Stiuso, P."Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab". Oncology Letters 9.3 (2015): 1327-1332.
Chicago
De Rosa, A., Zappavigna, S., Villa, M. R., Improta, S., Cesario, E., Mastrullo, L., Caraglia, M., Stiuso, P."Prognostic role of translocator protein and oxidative stress markers in chronic lymphocytic leukemia patients treated with bendamustine plus rituximab". Oncology Letters 9, no. 3 (2015): 1327-1332. https://doi.org/10.3892/ol.2014.2817
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