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Article

Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients

  • Authors:
    • Jing Wang
    • Yuhai Zhang
    • Shudi Xu
    • Weijie Li
    • Zhangqin Chen
    • Zhe Wang
    • Xinpeng Han
    • Yiling Zhao
    • Shengqing Li
  • View Affiliations / Copyright

    Affiliations: Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China, Department of Medical Statistics, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China, Department of Respiratory Medicine, Shaanxi Provincial Second People's Hospital, Xi'an, Shaanxi, P.R. China, Department of Pathology, Fourth Military Medical University, Xi'an, Shaanxi, P.R. China
  • Pages: 1266-1272
    |
    Published online on: January 2, 2015
       https://doi.org/10.3892/ol.2015.2842
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Abstract

The aim of the present study was to retrospectively assess the correlation between the expression levels of proteins involved in G2/M arrest signaling pathways in non‑small cell lung cancer (NSCLC) tissue, as determined by immunohistochemical (IHC) methods, and the overall survival of patients with advanced stage NSCLC. IHC analysis of advanced NSCLC specimens was used to determine the expression levels of proteins involved in G2/M arrest signaling pathways, including ataxia telangiectasia mutated (ATM) kinase, ataxia telangiectasia and Rad3‑related (ATR) kinase, checkpoint kinase (Chk) 1, Chk2, cell division cycle 25C (Cdc25C), total cyclin‑dependent kinase 1 (Cdk1) and active Cdk1 signaling pathways, the latter of which refers to dephospho‑Cdk1 (Tyr15) and phospho‑Cdk1 (Thr161). Patients were enrolled continuously and followed up for ≥2 years. Univariate analysis demonstrated that the protein expression levels of dephospho‑Cdk1 (P=0.015) and phospho‑Cdk1 (P=0.012) exhibited prognostic significance, while the expression of the other proteins was not significantly associated with patient survival (ATM, P=0.843; ATR, P=0.245; Chk1, P=0.341; Chk2, P=0.559; Cdc25C, P=0.649; total Cdk1, P=0.093). Furthermore, the patients with tumors exhibiting low expression levels of active Cdk1 survived significantly longer than those with tumors exhibiting high active Cdk1 expression levels (P<0.05). In addition, Cox regression analysis demonstrated that the expression of active Cdk1 [odds ratio (OR), 0.624; 95% confidence ratio (CI), 0.400‑0.973; P=0.038] and the pathological tumor‑node‑metastasis stage (OR, 0.515; 95% CI, 0.297‑0.894; P=0.018) were significant independent prognostic factors for NSCLC. Therefore, the results of the present study indicated that active Cdk1 protein is an independent prognostic factor for advanced NSCLC and may validate Cdk1 as a therapeutic target for advanced NSCLC patients.
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Copy and paste a formatted citation
Spandidos Publications style
Wang J, Zhang Y, Xu S, Li W, Chen Z, Wang Z, Han X, Zhao Y and Li S: Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients. Oncol Lett 9: 1266-1272, 2015.
APA
Wang, J., Zhang, Y., Xu, S., Li, W., Chen, Z., Wang, Z. ... Li, S. (2015). Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients. Oncology Letters, 9, 1266-1272. https://doi.org/10.3892/ol.2015.2842
MLA
Wang, J., Zhang, Y., Xu, S., Li, W., Chen, Z., Wang, Z., Han, X., Zhao, Y., Li, S."Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients". Oncology Letters 9.3 (2015): 1266-1272.
Chicago
Wang, J., Zhang, Y., Xu, S., Li, W., Chen, Z., Wang, Z., Han, X., Zhao, Y., Li, S."Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients". Oncology Letters 9, no. 3 (2015): 1266-1272. https://doi.org/10.3892/ol.2015.2842
Copy and paste a formatted citation
x
Spandidos Publications style
Wang J, Zhang Y, Xu S, Li W, Chen Z, Wang Z, Han X, Zhao Y and Li S: Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients. Oncol Lett 9: 1266-1272, 2015.
APA
Wang, J., Zhang, Y., Xu, S., Li, W., Chen, Z., Wang, Z. ... Li, S. (2015). Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients. Oncology Letters, 9, 1266-1272. https://doi.org/10.3892/ol.2015.2842
MLA
Wang, J., Zhang, Y., Xu, S., Li, W., Chen, Z., Wang, Z., Han, X., Zhao, Y., Li, S."Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients". Oncology Letters 9.3 (2015): 1266-1272.
Chicago
Wang, J., Zhang, Y., Xu, S., Li, W., Chen, Z., Wang, Z., Han, X., Zhao, Y., Li, S."Prognostic significance of G2/M arrest signaling pathway proteins in advanced non-small cell lung cancer patients". Oncology Letters 9, no. 3 (2015): 1266-1272. https://doi.org/10.3892/ol.2015.2842
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