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Article

Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells

  • Authors:
    • Sheng Li
    • Sheng Jiang
    • Wei Jiang
    • Yue Zhou
    • Xiu‑Yin Shen
    • Tao Luo
    • Ling‑Ping Kong
    • Hua‑Qiao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy and Neurobiology, Zhongshan School of Medicine, Sun Yat‑sen University, Guangzhou, Guangdong 510080, P.R. China
  • Pages: 1254-1260
    |
    Published online on: January 13, 2015
       https://doi.org/10.3892/ol.2015.2869
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Abstract

Crocetin is the main pharmacologically‑active component of saffron and has been considered as a promising candidate for cancer chemoprevention. The purpose of the present study was to investigate the anticancer effects of crocetin and the possible mechanisms of these properties in the esophageal squamous cell carcinoma cell line KYSE‑150. The KYSE‑150 cells were cultured in Dulbecco's modified Eagle's medium and incubated with 0, 12.5, 25, 50, 100 or 200 µmol/l crocetin for 48 h. Cell proliferation was measured using an MTT assay. Hoechst 33258 staining and observation under fluorescent microscopy were used to analyze the proapoptotic effects of crocetin. The migration rate was assessed by a wound‑healing assay. The cell cycle distribution was analyzed using flow cytometry analysis subsequent to propidium iodide staining. The expression of B-cell lymphoma-2-associated X protein (Bax) and cleaved caspase 3 was determined by western blot analysis. It was found that treatment of KYSE‑150 cells with crocetin for 48 h significantly inhibited the proliferation of the cells in a concentration‑dependent manner, and the inhibition of proliferation was associated with S phase arrest. Crocetin was also found to induce morphological changes and cell apoptosis in a dose‑dependent manner through increased expression of proapoptotic Bax and activated caspase 3. In addition, crocetin suppressed the migration of KYSE‑150 cells. The present study provides evidence that crocetin exerts a prominent chemopreventive effect against esophageal cancer through the inhibition of cell proliferation, migration and induction of apoptosis. These findings reveal that crocetin may be considered to be a promising future chemotherapeutic agent for esophageal cancer therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Li S, Jiang S, Jiang W, Zhou Y, Shen XY, Luo T, Kong LP and Wang HQ: Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells. Oncol Lett 9: 1254-1260, 2015.
APA
Li, S., Jiang, S., Jiang, W., Zhou, Y., Shen, X., Luo, T. ... Wang, H. (2015). Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells. Oncology Letters, 9, 1254-1260. https://doi.org/10.3892/ol.2015.2869
MLA
Li, S., Jiang, S., Jiang, W., Zhou, Y., Shen, X., Luo, T., Kong, L., Wang, H."Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells". Oncology Letters 9.3 (2015): 1254-1260.
Chicago
Li, S., Jiang, S., Jiang, W., Zhou, Y., Shen, X., Luo, T., Kong, L., Wang, H."Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells". Oncology Letters 9, no. 3 (2015): 1254-1260. https://doi.org/10.3892/ol.2015.2869
Copy and paste a formatted citation
x
Spandidos Publications style
Li S, Jiang S, Jiang W, Zhou Y, Shen XY, Luo T, Kong LP and Wang HQ: Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells. Oncol Lett 9: 1254-1260, 2015.
APA
Li, S., Jiang, S., Jiang, W., Zhou, Y., Shen, X., Luo, T. ... Wang, H. (2015). Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells. Oncology Letters, 9, 1254-1260. https://doi.org/10.3892/ol.2015.2869
MLA
Li, S., Jiang, S., Jiang, W., Zhou, Y., Shen, X., Luo, T., Kong, L., Wang, H."Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells". Oncology Letters 9.3 (2015): 1254-1260.
Chicago
Li, S., Jiang, S., Jiang, W., Zhou, Y., Shen, X., Luo, T., Kong, L., Wang, H."Anticancer effects of crocetin in human esophageal squamous cell carcinoma KYSE-150 cells". Oncology Letters 9, no. 3 (2015): 1254-1260. https://doi.org/10.3892/ol.2015.2869
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