Apoptosis induced in MCF-7 human breast cancer cells by 2',4'-dihydroxy-6-methoxy-3,5-dimethylchalcone isolated from Eugenia aquea Burm f. leaves

Subarnas,Anas; Diantini,Ajeng; Abdulah,Rizky; Zuhrotun,Ade; Hadisaputri,Yuni  E.; Puspitasari,Irma  M.; Yamazaki,Chiho; Kuwano,Hiroyuki; Koyama,Hiroshi

doi:10.3892/ol.2015.2981

May-2015 Volume 9 Issue 5

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May-2015 Volume 9 Issue 5

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Article

Apoptosis induced in MCF-7 human breast cancer cells by 2',4'-dihydroxy-6-methoxy-3,5-dimethylchalcone isolated from Eugenia aquea Burm f. leaves

Authors:
- Anas Subarnas
- Ajeng Diantini
- Rizky Abdulah
- Ade Zuhrotun
- Yuni E. Hadisaputri
- Irma M. Puspitasari
- Chiho Yamazaki
- Hiroyuki Kuwano
- Hiroshi Koyama
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Affiliations: Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, Indonesia, Department of Biological Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, West Java, Indonesia, Department of General Surgical Science, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan, Department of Public Health, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan
Pages: 2303-2306
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Published online on: February 24, 2015

https://doi.org/10.3892/ol.2015.2981
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Abstract

During a previous study that aimed to identify anticancer agents within primate‑consumed plants, the present group identified that Eugenia aquea (E. aquea) possessed potential as a source of anticancer agents. The ethanol extract of E. aquea leaves exhibited strong inhibitory activity against the proliferation of the human breast adenocarcinoma MCF‑7 cell line. The inhibition of proliferation was determined using an MTT assay. The present study was performed to isolate the active compound within the E. aquea leaves that generated the aforementioned activity, and resulted in the isolation of 2',4'‑dihydroxy‑6‑methoxy‑3,5‑dimethylchalcone, which was identified through the analysis of spectroscopic data. This compound was examined for its inhibitory activity against the MCF‑7 cell line using a MTT assay, and the ability of 2',4'‑dihydroxy‑6‑methoxy‑3,5‑dimethylchalcone to induce apoptosis through the activation of the poly(adenosine diphosphate‑ribose) polymerase (PARP) protein was also investigated. The results of the present study revealed that the isolated compound inhibited cell proliferation in a dose‑dependent manner, possessed an IC50 of 74.5 µg/ml (250 µM) and promoted apoptosis via the activation of PARP. It was concluded that these results indicated a requirement for additional investigations into 2',4'‑dihydroxy‑6‑methoxy‑3,5‑dimethylchalcone in order to provide a basis for the use of this compound in the management of cancer.

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