Expression and significance of glucose-regulated protein 78 in human osteosarcoma

  • Authors:
    • Yongkui Zhang
    • Nianhu Li
    • Dongli Wang
    • Yiqiang Chen
    • Gang Li
  • View Affiliations

  • Published online on: March 12, 2015     https://doi.org/10.3892/ol.2015.3030
  • Pages: 2268-2274
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Abstract

The present study aimed to investigate the expression of glucose-regulated protein 78 (GRP78) in osteosarcoma cells, and analyze the differences in expression between tumor and normal tissues, pre‑ and post‑chemotherapy patients and metastatic and non‑metastatic tumors. According to these results, the associations between the expression of GRP78 and tumor growth, metastasis and chemotherapeutics could be determined. Between 2007 and 2012, 60 patients who had been diagnosed with osteosarcoma were selected for the present study. Of these patients, 20 presented with non‑metastatic tumors and 40 with metastatic tumors, and 20 had been treated without chemotherapy and 40 with chemotherapy. In addition, 60 specimens obtained from adjacent normal tissues were collected for the control groups. Immunofluorescence staining was used to examine the expression of GRP78 in the different tissues. The total RNA and protein were extracted from crushed tissues and used in the reverse transcription polymerase chain reaction and western blot analysis. GRP78 was primarily located in the intracavity of the endoplasmic reticulum. The expression level of GRP78 in the tumor tissue was higher than that in the normal tissue surrounding the tumor (P<0.01). In addition, the level was higher in the metastatic tumors compared with the non‑metastatic tumors (P<0.05), and in the non‑chemotherapy‑treated patients compared with the chemotherapy‑treated patients (P<0.01). The expression level of GRP78 mRNA in the tumor tissue was higher than that in the normal tissue (P<0.01). Furthermore, the level was higher in the metastasis group than in the non‑metastasis group (P<0.05), and in the non‑chemotherapy group than in the chemotherapy group (P<0.01). The expression level of GRP78 protein was higher in the tumor tissue compared with the normal tissue (P<0.01), in the metastasis group compared with the non‑metastasis group (P<0.05), and in the non‑chemotherapy group compared with the chemotherapy group (P<0.01). In conclusion, the present study detected the expression of GRP78 in patients with osteosarcoma and revealed a higher expression level in the tumor tissues compared with the normal tissues around the tumor, in the metastasis group compared with the non‑metastasis group and in the non‑chemotherapy‑treated group compared with the chemotherapy‑treated group.
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May-2015
Volume 9 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang Y, Li N, Wang D, Chen Y and Li G: Expression and significance of glucose-regulated protein 78 in human osteosarcoma. Oncol Lett 9: 2268-2274, 2015
APA
Zhang, Y., Li, N., Wang, D., Chen, Y., & Li, G. (2015). Expression and significance of glucose-regulated protein 78 in human osteosarcoma. Oncology Letters, 9, 2268-2274. https://doi.org/10.3892/ol.2015.3030
MLA
Zhang, Y., Li, N., Wang, D., Chen, Y., Li, G."Expression and significance of glucose-regulated protein 78 in human osteosarcoma". Oncology Letters 9.5 (2015): 2268-2274.
Chicago
Zhang, Y., Li, N., Wang, D., Chen, Y., Li, G."Expression and significance of glucose-regulated protein 78 in human osteosarcoma". Oncology Letters 9, no. 5 (2015): 2268-2274. https://doi.org/10.3892/ol.2015.3030