Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26

Nishikawa,Shimpei; Konno,Masamitsu; Hamabe,Atsushi; Hasegawa,Shinichiro; Kano,Yoshihiro; Fukusumi,Takahito; Satoh,Taroh; Takiguchi,Shuji; Mori,Masaki; Doki,Yuichiro; Ishii,Hideshi

doi:10.3892/ol.2015.3063

May-2015 Volume 9 Issue 5

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May-2015 Volume 9 Issue 5

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Article

Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26

Authors:
- Shimpei Nishikawa
- Masamitsu Konno
- Atsushi Hamabe
- Shinichiro Hasegawa
- Yoshihiro Kano
- Takahito Fukusumi
- Taroh Satoh
- Shuji Takiguchi
- Masaki Mori
- Yuichiro Doki
- Hideshi Ishii
View Affiliations / Copyright

Affiliations: Department of Frontier Science for Cancer and Chemotherapy, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan, Department of Otorhinolaryngology‑Head and Neck Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan, Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka 565‑0871, Japan
Pages: 2361-2367
|
Published online on: March 20, 2015

https://doi.org/10.3892/ol.2015.3063
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Abstract

Cancer tissue is maintained by relatively small populations of cancer stem cells (CSCs), which are involved in chemotherapy resistance, recurrence and metastasis. As tumor tissues are comprised of various cells, studies of human clinical samples are important for the characterization of CSCs. In the present study, an expression profiling study was performed in which an anti‑cell surface marker antibody‑based array platform, a flow cytometry‑based cell separation technique and a tumorigenicity analysis in immunodeficient animals were utilized. These approaches revealed that the markers cluster of differentiation (CD)44 and CD26 facilitated the fractionation of surgically resected human gastric cancer (GC) cells into the following subset populations with distinct tumorigenic potentials: Highly tumorigenic CD26+CD44+ cells (6/6 mice formed tumors), moderately tumorigenic CD26+CD44‑ cells (5/6 mice formed tumors), and weakly or non‑tumorigenic CD26‑CD44‑ cells (2/6 mice formed tumors). Furthermore, exposure to 5‑fluorouracil significantly increased the proportion of CD26+ cells in vitro. The present study demonstrated that the combined expression of CD26 and CD44 presents a potential marker of human GC stem cells.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Surgically resected human tumors reveal the biological significance of the gastric cancer stem cell markers CD44 and CD26

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