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Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells

  • Authors:
    • Lin‑Lin Yan
    • Yuan‑Jiao Huang
    • Xiang Yi
    • Xue‑Min Yan
    • Yan Cai
    • Qin He
    • Zi‑Jian Han
  • View Affiliations / Copyright

    Affiliations: Medical Scientific Research Center, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, Department of Otolaryngology ‑ Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
    Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2534-2540
    |
    Published online on: March 31, 2015
       https://doi.org/10.3892/ol.2015.3090
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Abstract

The calcium-binding S100 proteins are involved in functions such as cell growth, differentiation, migration, adhesion and signal transduction. S100A8 and S100A9 are highly expressed in a variety of tumor cells, and are implicated in tumor development and progression. However, the role of S100A8 and S100A9 in nasopharyngeal carcinoma (NPC) cell migration is unclear. The present study investigated the effect of S100A8 and S100A9 on migration using a NPC cell line, CNE1. The CNE1 cells were transfected with S100A8 or S100A9 small interfering RNA (siRNA). Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect S100A8 and S100A9 gene expression. Following the downregulation of S100A8 or S100A9, the effects on cell migration were determined using wound‑healing assays. The expression of matrix metalloproteinase‑7 (MMP7), a member of the MMP family that is associated with tumor cell invasion and migration, was also detected by RT‑qPCR. S100A8 and S100A9 siRNAs effectively suppressed S100A8 and S100A9 gene expression, and substantially inhibited the migration of the CNE1 cells. In addition, MMP7 expression was reduced to varying extents in S100A8 and S100A9 siRNA-treated cells compared with controls. Thus, S100A8 and S100A9 promoted the migration of CNE1 NPC cells.
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1 

Sham JS, Wei WI, Zong YS, Choy D, Guo YQ, Luo Y, Lin ZX and Ng MH: Detection of subclinical nasopharyngeal carcinoma by fibreoptic endoscopy and multiple biopsy. Lancet. 335:371–374. 1990. View Article : Google Scholar : PubMed/NCBI

2 

Chang ET and Adami HO: The enigmatic epidemiology of nasopharyngeal carcinoma. Cancer Epidemiol Biomarkers Prev. 15:1765–1777. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Shin HR, Curado MP, Ferlay J, Heanue M, Edwards B and Storm H: Comparability and Quality of DataCancer Incidence in Five Continents. Parkin DM and Muir CS: IARC Sci Publ; pp. 45–173. 1992

4 

Nielsen NH, Mikkelsen F and Hansen JP: Nasopharyngeal cancer in Greenland. The incidence in an Arctic Eskimo population. Acta Pathol Microbiol Scand A. 85:850–858. 1977.PubMed/NCBI

5 

Yu MC and Yuan JM: Epidemiology of nasopharyngeal carcinoma. Semin Cancer Biol. 12:421–429. 2002. View Article : Google Scholar : PubMed/NCBI

6 

Gu AD, Mo HY, Bei JX, Xie YB, Chen LZ, Feng QS, Kang T and Zeng YX: Evaluation of antibodies against different Epstein-Barr virus nuclear antigen 1 peptides in diagnosis of nasopharyngeal carcinoma. Clin Vaccine Immunol. 16:592–593. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Chiesa F and De Paoli F: Distant metastases from nasopharyngeal cancer. ORL J Otorhinolaryngol Relat Spec. 63:214–216. 2001. View Article : Google Scholar : PubMed/NCBI

8 

Korndörfer IP, Brueckner F and Skerra A: The crystal structure of the human (S100A8/S100A9)2 heterotetramer, calprotectin, illustrates how conformational changes of interacting alpha-helices can determine specific association of two EF-hand proteins. J Mol Biol. 370:887–898. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Ott HW, Lindner H, Sarg B, Mueller-Holzner E, Abendstein B, Bergant A, Fessler S, Schwaerzler P, Zeimet A, Marth C, et al: Calgranulins in cystic fluid and serum from patients with ovarian carcinomas. Cancer Res. 63:7507–7514. 2003.PubMed/NCBI

10 

Donato R: S100: A multigenic family of calcium-modulated proteins of the EF-hand type with intracellular and extracellular functional roles. Int J Biochem Cell Biol. 33:637–668. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Kong JP, Ding F, Zhou CN, Wang XQ, Miao XP, Wu M and Liu ZH: Loss of myeloid-related proteins 8 and myeloid-related proteins 14 expression in human esophageal squamous cell carcinoma correlates with poor differentiation. World J Gastroenterol. 10:1093–1097. 2004.PubMed/NCBI

12 

Gebhardt C, Németh J, Angel P and Hess J: S100A8 and S100A9 in inflammation and cancer. Biochem Pharmacol. 72:1622–1631. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Salama I, Malone PS, Mihaimeed F and Jones JL: A review of the S100 proteins in cancer. Eur J Surg Oncol. 34:357–364. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Kim HJ, Kang HJ, Lee H, Lee ST, Yu MH, Kim H and Lee C: Identification of S100A8 and S100A9 as serological markers for colorectal cancer. J Proteome Res. 8:1368–1379. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Hermani A, Hess J, De Servi B, et al: Calcium-binding proteins S100A8 and S100A9 as novel diagnostic markers in human prostate cancer. Clin Cancer Res. 11:5146–5152. 2005. View Article : Google Scholar : PubMed/NCBI

16 

Saha A, Lee YC, Zhang Z, Chandra G, Su SB and Mukherjee AB: Lack of an endogenous anti-inflammatory protein in mice enhances colonization of B16F10 melanoma cells in the lungs. J Biol Chem. 285:10822–10831. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Yong HY and Moon A: Roles of calcium-binding proteins, S100A8 and S100A9, in invasive phenotype of human gastric cancer cells. Arch Pharm Res. 30:75–81. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Han RR, Huang YJ, Chen L, Xiao XL, Yi X, Cai HW and Wu YH: Determination of S100A8 and S100A9 protein in plasma of nasopharyngeal carcinoma patients and its clinical significance. Chin J Clin Lab Sci. 32:252–254. 2014.[(In Chinese)].

19 

Hermani A, De Servi B, Medunjanin S, Tessier PA and Mayer D: S100A8 and S100A9 activate MAP kinase and NF-kappaB signaling pathways and trigger translocation of RAGE in human prostate cancer cells. Exp Cell Res. 312:184–197. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Hiratsuka S, Watanabe A, Aburatani H and Maru Y: Tumour-mediated upregulation of chemoattractants and recruitment of myeloid cells predetermines lung metastasis. Nat Cell Biol. 8:1369–1375. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Kwon CH, Moon HJ, Park HJ, Choi JH and Park Y: S100A8 and S100A9 promotes invasion and migration through p38 mitogen-activated protein kinase-dependent NF-κB activation in gastric cancer cells. Mol Cells. 35:226–234. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Roy R, Yang J and Moses MA: Matrix metalloproteinases as novel biomarkers and potential therapeutic targets in human cancer. J Clin Oncol. 27:5287–5297. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Moon A, Yong HY, Song JI, Cukovic D, Salagrama S, Kaplan D, Putt D, Kim H, Dombkowski A and Kim HR: Global gene expression profiling unveils S100A8/A9 as candidate markers in H-ras-mediated human breast epithelial cell invasion. Mol Cancer Res. 6:1544–1553. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Duan L, Wu R, Ye L, Wang H, Yang X, Zhang Y, Chen X, Zuo G, Zhang Y, Weng Y, et al: S100A8 and S100A9 are associated with colorectal carcinoma progression and contribute to colorectal carcinoma cell survival and migration via Wnt/β-catenin pathway. PLoS One. 8:e620922013. View Article : Google Scholar : PubMed/NCBI

25 

Ichikawa M, Williams R, Wang L, Vogl T and Srikrishna G: S100A8/A9 activate key genes and pathways in colon tumor progression. Mol Cancer Res. 9:133–148. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yan LL, Huang YJ, Yi X, Yan XM, Cai Y, He Q and Han ZJ: Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells. Oncol Lett 9: 2534-2540, 2015.
APA
Yan, L., Huang, Y., Yi, X., Yan, X., Cai, Y., He, Q., & Han, Z. (2015). Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells. Oncology Letters, 9, 2534-2540. https://doi.org/10.3892/ol.2015.3090
MLA
Yan, L., Huang, Y., Yi, X., Yan, X., Cai, Y., He, Q., Han, Z."Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells". Oncology Letters 9.6 (2015): 2534-2540.
Chicago
Yan, L., Huang, Y., Yi, X., Yan, X., Cai, Y., He, Q., Han, Z."Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells". Oncology Letters 9, no. 6 (2015): 2534-2540. https://doi.org/10.3892/ol.2015.3090
Copy and paste a formatted citation
x
Spandidos Publications style
Yan LL, Huang YJ, Yi X, Yan XM, Cai Y, He Q and Han ZJ: Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells. Oncol Lett 9: 2534-2540, 2015.
APA
Yan, L., Huang, Y., Yi, X., Yan, X., Cai, Y., He, Q., & Han, Z. (2015). Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells. Oncology Letters, 9, 2534-2540. https://doi.org/10.3892/ol.2015.3090
MLA
Yan, L., Huang, Y., Yi, X., Yan, X., Cai, Y., He, Q., Han, Z."Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells". Oncology Letters 9.6 (2015): 2534-2540.
Chicago
Yan, L., Huang, Y., Yi, X., Yan, X., Cai, Y., He, Q., Han, Z."Effects of silencing S100A8 and S100A9 with small interfering RNA on the migration of CNE1 nasopharyngeal carcinoma cells". Oncology Letters 9, no. 6 (2015): 2534-2540. https://doi.org/10.3892/ol.2015.3090
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