Analysis of AC3-33 gene expression in multiple organ cancer and adjacent normal tissue by RNA in situ hybridization

Hu,Fen; Yang,Shaoqing; Lv,Shaobo; Peng,Yan; Meng,Lijun; Gou,Lixia; Zhang,Xiujun

doi:10.3892/ol.2015.3112

June-2015 Volume 9 Issue 6

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June-2015 Volume 9 Issue 6

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Article

Analysis of AC3-33 gene expression in multiple organ cancer and adjacent normal tissue by RNA in situ hybridization

Authors:
- Fen Hu
- Shaoqing Yang
- Shaobo Lv
- Yan Peng
- Lijun Meng
- Lixia Gou
- Xiujun Zhang
View Affiliations / Copyright

Affiliations: College of Life Sciences, College of Psychology, Hebei United University, Tangshan, Hebei 063000, P.R. China, Department of Environment and Chemical Engineering, Tangshan College, Tangshan, Hebei 063000, P.R. China
Pages: 2795-2798
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Published online on: April 14, 2015

https://doi.org/10.3892/ol.2015.3112
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Abstract

The AC3-33 gene encodes a secretory protein that can inhibit Elk1 transcriptional activity via the ERK1/2 pathway. In the current study, in situ RNA hybridization was used to detect the AC3‑33 gene expression in multiple organ cancer and cancer‑adjacent normal tissue. The results showed that the expression level of AC3‑33 varies across different tissues. AC3‑33 exhibited positive expression in squamous cell carcinoma of the esophagus, adenocarcinoma of the rectum, hepatocellular carcinoma, squamous cell carcinoma (SCC) of the lung, cancer‑adjacent normal hepatic tissue, clear cell carcinoma of the kidney, invasive ductal carcinoma of the breast, SCC of the uterine cervix and cancer‑adjacent normal kidney tissue. Negative expression of AC3‑33 was observed in adenocarcinoma of the stomach and colon, cancer‑adjacent normal esophageal tissue, cancer‑adjacent normal gastric tissue, cancer‑adjacent normal colon tissue, cancer‑adjacent normal rectal tissue, serous adenocarcinoma of the ovary and cancer‑adjacent normal ovarian tissue. However, the expression of AC3‑33 in cancer adjacent normal breast tissue was partially positive. In conclusion, the AC3‑33 gene does exhibit positive expression in certain carcinomas, which may indicate that AC3-33 has a significant involvement in the development and progression of these carcinomas.

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1	Zhang X, Ma X, Xue Y, et al: Prokaryotic expression and characterization of human AC3-33 protein. Front Biosci (Elite Ed). 2:1134–1142. 2010. View Article : Google Scholar : PubMed/NCBI
2	Angel P and Karin M: The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation. Biochim Biophys Acta. 1072:129–157. 1991.PubMed/NCBI
3	Hao D, Gao P, Liu P, et al: AC3-33, a novel secretory protein, inhibits Elk1 transcriptional activity via ERK pathway. Mol Biol Rep. 38:1375–1382. 2011. View Article : Google Scholar : PubMed/NCBI
4	Liu P, Deng WW, Gao P, et al: Molecular cloning and preliminary function study of a novel human gene AC3-33 related to suppress AP-1 activity. Yi Chuan. 30:575–582. 2008.(In Chinese). View Article : Google Scholar : PubMed/NCBI
5	Bollschweiler E and Holscher AH: Carcinoma of the esophagus-actual epidemiology in Germany. Onkologie. 24:180–184. 2001.(In German). View Article : Google Scholar : PubMed/NCBI
6	Suita S, Zaizen Y, Kaneko M, et al: What is the benefit of aggressive chemotherapy for advanced neuroblastoma with N-myc amplification? A report from the Japanese study group for the treatment of advanced neuroblastoma. J Pediatr Surg. 29:746–750. 1994. View Article : Google Scholar : PubMed/NCBI
7	Kitagawa Y, Ueda M, Ando N, Shinozawa Y, Shimizu N and Abe O: Significance of int-2/hst-1 coamplification as a prognostic factor in patients with esophageal squamous carcinoma. Cancer Res. 51:1504–1508. 1991.PubMed/NCBI
8	Shinozaki H, Ozawa S, Ando N, et al: Cyclin D1 amplification as a new predictive classification for squamous cell carcinoma of the esophagus, adding gene information. Clin Cancer Res. 2:1155–1161. 1996.PubMed/NCBI
9	Yamasaki M, Miyata H, Fujiwara Y, et al: p53 genotype predicts response to chemotherapy in patients with squamous cell carcinoma of the esophagus. Ann Surg Oncol. 17:634–642. 2010. View Article : Google Scholar : PubMed/NCBI
10	National Cancer Institute, . Colon Cancer Treatment (PDQ®). http://www.cancer.gov/cancertopics/pdq/treatment/colon/Patient/page1/AllPagesAccessed. 29–June. 2014
11	Comprehensive molecular characterization of human colon and rectal cancer. Nature. 487:330–337. 2012. View Article : Google Scholar : PubMed/NCBI
12	Olson RM, Perencevich NP, Malcolm AW, Chaffey JT and Wilson RE: Patterns of recurrence following curative resection of adenocarcinoma of the colon and rectum. Cancer. 45:2969–2974. 1980. View Article : Google Scholar : PubMed/NCBI
13	Ratto C, Sofo L, Ippoliti M, Merico M, Doglietto GB and Crucitti F: Prognostic factors in colorectal cancer. Literature review for clinical application. Dis Colon Rectum. 41:1033–1049. 1998. View Article : Google Scholar : PubMed/NCBI
14	Jurach MT, Meurer L and Moreira LF: Expression of the p53 protein and clinical and pathologic correlation in adenocarcinoma of the rectum. Arq Gastroenterol. 43:9–14. 2016.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Analysis of AC3-33 gene expression in multiple organ cancer and adjacent normal tissue by RNA in situ hybridization

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