Association between LMP2 and LMP7 gene polymorphisms and the risk of gastric cancer: A case-control study

Ma,Xiang; Yang,Chao; Tang,Ran; Xu,Zekuan; Zhang,Zhihong; Wang,Younan; Zhang,Jingjing; Yang,Li

doi:10.3892/ol.2015.3154

Association between LMP2 and LMP7 gene polymorphisms and the risk of gastric cancer: A case-control study

Authors:
- Xiang Ma
- Chao Yang
- Ran Tang
- Zekuan Xu
- Zhihong Zhang
- Younan Wang
- Jingjing Zhang
- Li Yang
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Affiliations: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China, Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
Published online on: April 27, 2015 https://doi.org/10.3892/ol.2015.3154
Pages: 509-517

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Abstract

The integrality of low molecular weight protein (LMP)2/LMP7 function plays an important role in the processing of GC cell antigens. The purpose of the present hospital-based case-control study was to estimate the effect of polymorphisms in the LMP2 and LMP7 genes on the risk of GC. Polymerase chain reaction‑restriction fragment length polymorphism analysis was used to distinguish the Arg to His substitution at codon 60 of LMP2 (LMP2‑60) and the Gln to Lys substitution at codon 145 of LMP7 (LMP7‑145) in 502 gastric cancer patients and 502 age and gender‑matched cancer‑free control individuals. The Lys allele of the LMP7‑145 variant was more frequent in GC patients compared with control individuals [P=0.004; adjusted odds ratio (OR), 1.39; 95% confidence interval (CI), 1.11‑1.74]. The Gln/Lys and Lys/Lys genotypes increased the risk of GC compared with the Gln/Gln genotype (P=0.049 and P=0.041, respectively; adjusted OR, 1.32 and 2.13, respectively; 95% CI, 1.00‑1.73 and 1.03‑4.39, respectively). Compared with the Gln/Gln genotype, the LMP7‑145 Gln/Lys and Lys/Lys variants of the LMP7 gene were also associated with increased susceptibility to GC (P=0.017; adjusted OR, 1.38; 95% CI, 1.06‑1.80). Haplotype analysis revealed that the LMP2 (Arg)‑LMP7 (Lys) haplotype was associated with increased risk of GC (P=0.013, adjusted OR=1.34, 95% CI=1.06‑1.70). Stratified analysis revealed that the association between the risk of GC and the variant genotypes of LMP7‑145 was stronger in older individuals (>59 years), males and non‑smokers. However, no association between the LMP2‑60 polymorphism and the risk of GC was observed. The present results suggest that the LMP7‑145 genetic variant contributes to increased susceptibility to GC, and the Lys allele is an independent risk factor for GC.

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