Effect of hyperthermic CO2-treated dendritic cell-derived exosomes on the human gastric cancer AGS cell line

Wang,Jinlin; Wang,Zhiyong; Mo,Yanxia; Zeng,Zhaohui; Wei,Pei; Li,Tao

doi:10.3892/ol.2015.3155

Effect of hyperthermic CO2-treated dendritic cell-derived exosomes on the human gastric cancer AGS cell line

Authors:
- Jinlin Wang
- Zhiyong Wang
- Yanxia Mo
- Zhaohui Zeng
- Pei Wei
- Tao Li
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Affiliations: Department of General Surgery, Dongguan People's Hospital, Dongguan, Guangdong 523059, P.R. China, Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical College, Dongguan, Guangdong 523808, P.R. China
Published online on: April 27, 2015 https://doi.org/10.3892/ol.2015.3155
Pages: 71-76
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to determine the antitumor effects of hyperthermic CO2 (HT-CO2)-treated dendritic cell (DC)-derived exosomes (Dex) on human gastric cancer AGS cells. Mouse‑derived DCs were incubated in HT‑CO2 at 43˚C for 4 h. The exosomes in the cell culture supernatant were then isolated. Cell proliferation was analyzed using the cell counting kit‑8 (CCK‑8) assay. Cell apoptosis was observed using flow cytometry, Hoechst 33258 staining and the analysis of caspase‑3 activity. In addition, the proliferation of tumor cells was evaluated in xenotransplant nude mice. HT‑CO2 markedly inhibited cell proliferation, as assessed by the CCK‑8 assay, and also induced apoptosis in a time‑dependent manner, as demonstrated by Annexin V/propidium iodide flow cytometry, caspase‑3 activity and morphological analysis using Hoechst fluorescent dye. It was also revealed that HT‑CO2‑treated Dex decreased the expression of heat shock protein 70 and inhibited tumor growth in nude mice. In conclusion, HT‑CO2 exerted an efficacious immune‑enhancing effect on DCs. These findings may provide a novel strategy for the elimination of free cancer cells during laparoscopic resection. However, the potential cellular mechanisms underlying this process require further investigation.

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