Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development

Liu,Lan‑Mei; Sun,Hong‑Ai; Li,Xiao; Chen,Yang; Wei,Bao‑Fu; Li,Xiu‑Ju

doi:10.3892/ol.2015.3163

July-2015 Volume 10 Issue 1

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July-2015 Volume 10 Issue 1

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Article

Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development

Authors:
- Lan‑Mei Liu
- Hong‑Ai Sun
- Xiao Li
- Yang Chen
- Bao‑Fu Wei
- Xiu‑Ju Li
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Affiliations: Department of Orthopedics, Linyi People's Hospital, Linyi, Shangdong 276000, P.R. China, Red Cross Blood Center of Linyi City, Linyi, Shangdong 276001, P.R. China, Department of Orthopedics, Shandong Provincial Hospital, Jinan, Shandong 250021, P.R. China, Department of General Surgery, Linyi Cancer Hospital, Linyi, Shangdong 276000, P.R. China
Pages: 273-276
|
Published online on: April 29, 2015

https://doi.org/10.3892/ol.2015.3163
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Abstract

Osteosarcoma (OS) is an aggressive primary bone cancer that usually affects children and young adolescents. Previous studies have demonstrated the implications of a small sub‑population of cancer stem cells on treatment failure and tumor recurrence. The present study analyzed the characteristic features of the stem‑like cells within the human OS‑55 cell line. It was identified that 2.3% of the OS‑55 cells were cancer stem‑like side population (SP) cells. Following treatment with verapamil, the population of SP cells was reduced to 0.7%. The sphere formation assay revealed that the OS cells were able to rapidly form tumor spheres (also known as sarcospheres). Immunofluorescence analysis identified that the OS‑55 cells expressed the cluster of differentiation 44, octamer‑binding transcription factor‑3/4A and Nanog stem cell surface markers. The results of the present study suggest that, as with other tumors, OS also contains a sub‑population of cancer stem‑like cells, which may have important implications in cancer diagnosis and treatment.

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