Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development

  • Authors:
    • Lan‑Mei Liu
    • Hong‑Ai Sun
    • Xiao Li
    • Yang Chen
    • Bao‑Fu Wei
    • Xiu‑Ju Li
  • View Affiliations

  • Published online on: April 29, 2015     https://doi.org/10.3892/ol.2015.3163
  • Pages: 273-276
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Abstract

Osteosarcoma (OS) is an aggressive primary bone cancer that usually affects children and young adolescents. Previous studies have demonstrated the implications of a small sub‑population of cancer stem cells on treatment failure and tumor recurrence. The present study analyzed the characteristic features of the stem‑like cells within the human OS‑55 cell line. It was identified that 2.3% of the OS‑55 cells were cancer stem‑like side population (SP) cells. Following treatment with verapamil, the population of SP cells was reduced to 0.7%. The sphere formation assay revealed that the OS cells were able to rapidly form tumor spheres (also known as sarcospheres). Immunofluorescence analysis identified that the OS‑55 cells expressed the cluster of differentiation 44, octamer‑binding transcription factor‑3/4A and Nanog stem cell surface markers. The results of the present study suggest that, as with other tumors, OS also contains a sub‑population of cancer stem‑like cells, which may have important implications in cancer diagnosis and treatment.
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July-2015
Volume 10 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu LM, Sun HA, Li X, Chen Y, Wei BF and Li XJ: Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development. Oncol Lett 10: 273-276, 2015
APA
Liu, L., Sun, H., Li, X., Chen, Y., Wei, B., & Li, X. (2015). Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development. Oncology Letters, 10, 273-276. https://doi.org/10.3892/ol.2015.3163
MLA
Liu, L., Sun, H., Li, X., Chen, Y., Wei, B., Li, X."Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development". Oncology Letters 10.1 (2015): 273-276.
Chicago
Liu, L., Sun, H., Li, X., Chen, Y., Wei, B., Li, X."Cluster of differentiation 44- and octamer-binding transcription factor-4-positive stem-like osteosarcoma cells involved in tumor development". Oncology Letters 10, no. 1 (2015): 273-276. https://doi.org/10.3892/ol.2015.3163