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Article Open Access

Acquired resistance to decitabine and cross-resistance to gemcitabine during the long-term treatment of human HCT116 colorectal cancer cells with decitabine

  • Authors:
    • Mika Hosokawa
    • Mai Saito
    • Aiko Nakano
    • Sakura Iwashita
    • Ayano Ishizaka
    • Kumiko Ueda
    • Seigo Iwakawa
  • View Affiliations / Copyright

    Affiliations: Department of Pharmaceutics, Kobe Pharmaceutical University, Higashinada‑ku, Kobe 658‑8558, Japan
    Copyright: © Hosokawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 761-767
    |
    Published online on: May 22, 2015
       https://doi.org/10.3892/ol.2015.3253
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Abstract

The aim of the present study was to determine the effects of long-term exposure of decitabine (DAC) to HCT116 colorectal cancer (CRC) cells on the acquisition of resistance to DAC as well as cross-resistance to anticancer drugs used for CRC or other epigenetic modifiers. In the present study, DAC‑resistant HCT116 CRC cells were established through long‑term treatment with increasing concentrations of DAC (10 to 540 nM); and the cross‑resistance to other drugs was subsequently examined. DAC‑resistant HCT116 cells were obtained following a 104‑day treatment with DAC, including DAC‑free intervals. The results demonstrated that the IC50 value of DAC was increased ~100‑fold in DAC‑resistant HCT116 cells. Messenger (m)RNA expression of secreted frizzed‑related protein 1 (SFRP1), which is regulated by DNA methylation, was not detected in DAC‑resistant cells; however, SFRP1 mRNA was present in HCT116 cells treated with DAC for 52 days. DNA methyltransferase 1 (DNMT1) protein levels were slightly decreased until day 81 and then returned to control levels in DAC‑resistant cells. Further experiments using DAC‑resistant HCT116 cells revealed that these cells exhibited cross‑resistance to gemcitabine (Gem); however, cross-resistance was not observed for other DNMT inhibitors (azacitidine and zebularine), histone deacetylase inhibitors (trichostatin A, vorinostat and valproic acid) or anticancer drugs for CRC (5‑fluorouracil, irinotecan and oxaliplatin). Furthermore, the protein expression levels of cytidine deaminase (CDA) were increased, while those of deoxycytidine kinase (dCK) were decreased in DAC‑resistant HCT116 cells; by contrast, the mRNA expression levels for these proteins were not significantly altered. In conclusion, the results of the present study indicated that the long‑term treatment of HCT116 cells with DAC led to the acquisition of resistance to both DAC and Gem. In addition, these results may be partly attributed to changes in CDA and/or dCK, which are involved in metabolic pathways common to these two drugs.
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1 

Sarkar S, Horn G, Moulton K, et al: Cancer development, progression, and therapy: An epigenetic overview. Int J Mol Sci. 14:21087–21113. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Ishikawa T: Novel therapeutic strategies using hypomethylating agents in the treatment of myelodysplastic syndrome. Int J Clin Oncol. 19:10–15. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Foulks JM, Parnell KM, Nix RN, et al: Epigenetic drug discovery: Targeting DNA methyltransferases. J Biomol Screen. 17:2–17. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Christman JK: 5-Azacytidine and 5-aza-2′-deoxycytidine as inhibitors of DNA methylation: Mechanistic studies and their implications for cancer therapy. Oncogene. 21:5483–5495. 2002. View Article : Google Scholar : PubMed/NCBI

5 

Pluchino KM, Hall MD, Goldsborough AS, et al: Collateral sensitivity as a strategy against cancer multidrug resistance. Drug Resist Updat. 15:98–105. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Stordal B, Pavlakis N and Davey R: Oxaliplatin for the treatment of cisplatin-resistant cancer: A systematic review. Cancer Treat Rev. 33:347–357. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Fedier A, Dedes KJ, Imesch P, et al: The histone deacetylase inhibitors suberoylanilide hydroxamic (Vorinostat) and valproic acid induce irreversible and MDR1-independent resistance in human colon cancer cells. Int J Oncol. 31:633–641. 2007.PubMed/NCBI

8 

Dedes KJ, Dedes I, Imesch P, et al: Acquired vorinostat resistance shows partial cross-resistance to ‘second-generation’ HDAC inhibitors and correlates with loss of histone acetylation and apoptosis but not with altered HDAC and HAT activities. Anticancer Drugs. 20:321–333. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Imesch P, Dedes KJ, Furlato M, Fink D and Fedier A: MLH1 protects from resistance acquisition by the histone deacetylase inhibitor trichostatin A in colon tumor cells. Int J Oncol. 35:631–640. 2009.PubMed/NCBI

10 

Qin T, Castoro R, El Ahdab S, Jelinek J, Wang X, Si J, Shu J, He R, Zhang N, Chung W, Kantarjian HM and Issa JP: Mechanisms of resistance to decitabine in the myelodysplastic syndrome. PLoS One. 6:e233722011. View Article : Google Scholar : PubMed/NCBI

11 

Qin T, Jelinek J, Si J, Shu J and Issa JP: Mechanisms of resistance to 5-aza-2′-deoxycytidine in human cancer cell lines. Blood. 113:659–667. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Sripayap P, Nagai T, Uesawa M, Kobayashi H, Tsukahara T, Ohmine K, Muroi K and Ozawa K: Mechanisms of resistance to azacitidine in human leukemia cell lines. Exp Hematol. 42:294–306. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Mahfouz RZ, Jankowska A, Ebrahem Q, Gu X, Visconte V, Tabarroki A, Terse P, Covey J, Chan K, Ling Y, Engelke KJ, et al: Increased CDA expression/activity in males contributes to decreased cytidine analog half-life and likely contributes to worse outcomes with 5-azacytidine or decitabine therapy. Clin Cancer Res. 19:938–948. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Damaraju VL, Mowles D, Yao S, Ng A, Young JD, Cass CE and Tong Z: Role of human nucleoside transporters in the uptake and cytotoxicity of azacitidine and decitabine. Nucleosides Nucleotides Nucleic Acids. 31:236–255. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Hummel-Eisenbeiss J, Hascher A, Hals PA, Sandvold ML, Müller-Tidow C, Lyko F and Rius M: The role of human equilibrative nucleoside transporter 1 on the cellular transport of the DNA methyltransferase inhibitors 5-azacytidine and CP-4200 in human leukemia cells. Mol Pharmacol. 84:438–450. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Arimany-Nardi C, Errasti-Murugarren E, Minuesa G, Martinez-Picado J, Gorboulev V, Koepsell H and Pastor-Anglada M: Nucleoside transporters and human organic cation transporter 1 determine the cellular handling of DNA-methyltransferase inhibitors. Br J Pharmacol. 171:3868–3880. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Cowan LA, Talwar S and Yang AS: Will DNA methylation inhibitors work in solid tumors? A review of the clinical experience with azacitidine and decitabine in solid tumors. Epigenomics. 2:71–86. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Azad N, Zahnow CA, Rudin CM and Baylin SB: The future of epigenetic therapy in solid tumours - lessons from the past. Nat Rev Clin Oncol. 10:256–266. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Flis S, Gnyszka A and Flis K: DNA methyltransferase inhibitors improve the effect of chemotherapeutic agents in SW48 and HT-29 colorectal cancer cells. PLoS One. 9:e923052014. View Article : Google Scholar : PubMed/NCBI

20 

Ikehata M, Ogawa M, Yamada Y, Tanaka S, Ueda K and Iwakawa S: Different effects of epigenetic modifiers on the cytotoxicity induced by 5-fluorouracil, irinotecan or oxaliplatin in colon cancer cells. Biol Pharm Bull. 37:67–73. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Baylin SB and Ohm JE: Epigenetic gene silencing in cancer - a mechanism for early oncogenic pathway addiction? Nat Rev Cancer. 6:107–116. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Ahmed D, Eide PW, Eilertsen IA, Danielsen SA, Eknæs M, Hektoen M, Lind GE and Lothe RA: Epigenetic and genetic features of 24 colon cancer cell lines. Oncogenesis. 2:e712013. View Article : Google Scholar : PubMed/NCBI

23 

Issa JP: CpG island methylator phenotype in cancer. Nat Rev Cancer. 4:988–993. 2004. View Article : Google Scholar : PubMed/NCBI

24 

Kakumoto M, Takara K, Sakaeda T, Tanigawara Y, Kita T and Okumura K: MDR1-mediated interaction of digoxin with antiarrhythmic or antianginal drugs. Biol Pharm Bull. 25:1604–1607. 2002. View Article : Google Scholar : PubMed/NCBI

25 

de Jonge HJ, Fehrmann RS, de Bont ES, Hofstra RM, Gerbens F, Kamps WA, de Vries EG, van der Zee AG, te Meerman GJ and ter Elst A: Evidence based selection of housekeeping genes. PLoS One. 2:e8982007. View Article : Google Scholar : PubMed/NCBI

26 

Wong ML and Medrano JF: Real-time PCR for mRNA quantitation. Biotechniques. 39:75–85. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Suzuki H, Watkins DN, Jair KW, Schuebel KE, Markowitz SD, Chen WD, Pretlow TP, Yang B, Akiyama Y, Van Engeland M, Toyota M, et al: Epigenetic inactivation of SFRP genes allows constitutive WNT signaling in colorectal cancer. Nat Genet. 36:417–422. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Ueno H, Kiyosawa K and Kaniwa N: Pharmacogenomics of gemcitabine: Can genetic studies lead to tailor-made therapy? Br J Cancer. 97:145–151. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Lyons J, Bayar E, Fine G, McCullar M, Rolens R, Rubinfeld J and Rosenfeld C: Decitabine: Development of a DNA methyltransferase inhibitor for hematological malignancies. Curr Opin Investig Drugs. 4:1442–1450. 2003.PubMed/NCBI

30 

Kihslinger JE and Godley LA: The use of hypomethylating agents in the treatment of hematologic malignancies. Leuk Lymphoma. 48:1676–1695. 2007. View Article : Google Scholar : PubMed/NCBI

31 

Robak T: New nucleoside analogs for patients with hematological malignancies. Expert Opin Investig Drugs. 20:343–359. 2011. View Article : Google Scholar : PubMed/NCBI

32 

Qiu X, Hother C, Ralfkiær UM, Søgaard A, Lu Q, Workman CT, Liang G, Jones PA and Grønbæk K: Equitoxic doses of 5-azacytidine and 5-aza-2′deoxycytidine induce diverse immediate and overlapping heritable changes in the transcriptome. PLoS One. 5:e129942010. View Article : Google Scholar : PubMed/NCBI

33 

Cashen AF, Shah AK, Todt L, Fisher N and DiPersio J: Pharmacokinetics of decitabine administered as a 3-h infusion to patients with acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS). Cancer Chemother Pharmacol. 61:759–766. 2008. View Article : Google Scholar : PubMed/NCBI

34 

Marquez VE, Kelley JA, Agbaria R, Ben-Kasus T, Cheng JC, Yoo CB and Jones PA: Zebularine: A unique molecule for an epigenetically based strategy in cancer chemotherapy. Ann NY Acad Sci. 1058:246–254. 2005. View Article : Google Scholar : PubMed/NCBI

35 

Panczyk M: Pharmacogenetics research on chemotherapy resistance in colorectal cancer over the last 20 years. World J Gastroenterol. 20:9775–827. 2014. View Article : Google Scholar : PubMed/NCBI

36 

Damia G and Broggini M: Cell cycle checkpoint proteins and cellular response to treatment by anticancer agents. Cell Cycle. 3:46–50. 2004. View Article : Google Scholar : PubMed/NCBI

37 

Mini E, Nobili S, Caciagli B, Landini I and Mazzei T: Cellular pharmacology of gemcitabine. Ann Oncol. 17:v7–v12. 2006. View Article : Google Scholar : PubMed/NCBI

38 

Kahramanoğullari O, Fantaccini G, Lecca P, Morpurgo D and Priami C: Algorithmic modeling quantifies the complementary contribution of metabolic inhibitions to gemcitabine efficacy. PLoS One. 7:e501762012. View Article : Google Scholar : PubMed/NCBI

39 

Ohmine K, Kawaguchi K, Ohtsuki S, Motoi F, Egawa S, Unno M and Terasaki T: Attenuation of phosphorylation by deoxycytidine kinase is key to acquired gemcitabine resistance in a pancreatic cancer cell line: Targeted proteomic and metabolomic analyses in PK9 cells. Pharm Res. 29:2006–2016. 2012. View Article : Google Scholar : PubMed/NCBI

40 

Hodge LS, Taub ME and Tracy TS: The deaminated metabolite of gemcitabine, 2′,2′-difluorodeoxyuridine, modulates the rate of gemcitabine transport and intracellular phosphorylation via deoxycytidine kinase. Drug Metab Dispos. 39:2013–2016. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hosokawa M, Saito M, Nakano A, Iwashita S, Ishizaka A, Ueda K and Iwakawa S: Acquired resistance to decitabine and cross-resistance to gemcitabine during the long-term treatment of human HCT116 colorectal cancer cells with decitabine. Oncol Lett 10: 761-767, 2015.
APA
Hosokawa, M., Saito, M., Nakano, A., Iwashita, S., Ishizaka, A., Ueda, K., & Iwakawa, S. (2015). Acquired resistance to decitabine and cross-resistance to gemcitabine during the long-term treatment of human HCT116 colorectal cancer cells with decitabine. Oncology Letters, 10, 761-767. https://doi.org/10.3892/ol.2015.3253
MLA
Hosokawa, M., Saito, M., Nakano, A., Iwashita, S., Ishizaka, A., Ueda, K., Iwakawa, S."Acquired resistance to decitabine and cross-resistance to gemcitabine during the long-term treatment of human HCT116 colorectal cancer cells with decitabine". Oncology Letters 10.2 (2015): 761-767.
Chicago
Hosokawa, M., Saito, M., Nakano, A., Iwashita, S., Ishizaka, A., Ueda, K., Iwakawa, S."Acquired resistance to decitabine and cross-resistance to gemcitabine during the long-term treatment of human HCT116 colorectal cancer cells with decitabine". Oncology Letters 10, no. 2 (2015): 761-767. https://doi.org/10.3892/ol.2015.3253
Copy and paste a formatted citation
x
Spandidos Publications style
Hosokawa M, Saito M, Nakano A, Iwashita S, Ishizaka A, Ueda K and Iwakawa S: Acquired resistance to decitabine and cross-resistance to gemcitabine during the long-term treatment of human HCT116 colorectal cancer cells with decitabine. Oncol Lett 10: 761-767, 2015.
APA
Hosokawa, M., Saito, M., Nakano, A., Iwashita, S., Ishizaka, A., Ueda, K., & Iwakawa, S. (2015). Acquired resistance to decitabine and cross-resistance to gemcitabine during the long-term treatment of human HCT116 colorectal cancer cells with decitabine. Oncology Letters, 10, 761-767. https://doi.org/10.3892/ol.2015.3253
MLA
Hosokawa, M., Saito, M., Nakano, A., Iwashita, S., Ishizaka, A., Ueda, K., Iwakawa, S."Acquired resistance to decitabine and cross-resistance to gemcitabine during the long-term treatment of human HCT116 colorectal cancer cells with decitabine". Oncology Letters 10.2 (2015): 761-767.
Chicago
Hosokawa, M., Saito, M., Nakano, A., Iwashita, S., Ishizaka, A., Ueda, K., Iwakawa, S."Acquired resistance to decitabine and cross-resistance to gemcitabine during the long-term treatment of human HCT116 colorectal cancer cells with decitabine". Oncology Letters 10, no. 2 (2015): 761-767. https://doi.org/10.3892/ol.2015.3253
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