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Article

Tumstatin induces apoptosis mediated by Fas signaling pathway in oral squamous cell carcinoma SCC-VII cells

  • Authors:
    • Jeon Hwang‑Bo
    • Jong‑Hwa Park
    • In Sik Chung
  • View Affiliations / Copyright

    Affiliations: Department of Genetic Engineering and Graduate School of Biotechnology, Kyung Hee University, Yongin, Gyeonggi 446‑701, Republic of Korea
  • Pages: 1016-1022
    |
    Published online on: May 25, 2015
       https://doi.org/10.3892/ol.2015.3261
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Abstract

Oral squamous cell carcinoma is a cancer originating in the tissues lining the mouth and lips. The present study investigated the effects of recombinant tumstatin, an anti‑angiogenic agent with distinct antitumor activity, on oral squamous cell carcinoma SCC-VII cells. Apoptosis was characterized by YO-PRO-1 staining, sub-G1 population, and DNA fragmentation analysis. Apoptotic mechanism of tumstatin was also investigated. The antitumor activity of tumstatin was further evaluated using an SCC-VII animal model. Recombinant tumstatin was found to decrease the viability of SCC‑VII cells in a dose‑dependent manner. The number of cells stained with the apoptotic marker YO‑PRO‑1, the sub‑G1 cell population and the level of apoptotic DNA fragmentation increased in the SCC‑VII cells following treatment with recombinant tumstatin. In addition, recombinant tumstatin treatment increased the expression of the Fas gene at the transcript and protein levels, and the inhibition of cell viability by recombinant tumstatin was suppressed by a neutralizing anti‑Fas antibody. Furthermore, treatment with recombinant tumstatin decreased the volume and weight of tumors in C3H/HeJ mice implanted with SCC‑VII cells. In conclusion, the results indicated that tumstatin induced apoptosis that is mediated by the Fas signaling pathway in SCC‑VII cells and inhibited tumor growth in an SCC-VII animal model.
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1 

Koch WM, Stafford E and Bajaj G: Cancer of the oral cavity. Part A: General principles and managementHead and neck cancer: A multidisciplinary approach. Harrison LB, Sessions RB and Hong WK: 3rd. Lippincott Williams and Wilkins; Philadelphia, PA: pp. 250–265. 2009

2 

Feller L and Lemmer J: Oral squamous cell carcinoma: Epidemiology, clinical presentation and treatment. J Cancer Ther. 3:263–268. 2012. View Article : Google Scholar

3 

Raff MC, Barres BA, Burne JF, Coles HS, Ishizaki Y and Jacobson MD: Programmed cell death and the control of cell survival: Lessons from the nervous system. Science. 262:695–700. 1993. View Article : Google Scholar : PubMed/NCBI

4 

Wyllie AH, Kerr JF and Currie AR: Cell death: The significance of apoptosis. Int Rev Cytol. 68:251–306. 1980.PubMed/NCBI

5 

Kaufmann SH and Earnshaw WC: Induction of apoptosis by cancer chemotherapy. Exp Cell Res. 256:42–49. 2000. View Article : Google Scholar : PubMed/NCBI

6 

King KL and Cidlowski JA: Cell cycle regulation and apoptosis. Annu Rev Physiol. 60:601–617. 1998. View Article : Google Scholar : PubMed/NCBI

7 

Boedefeld WM 2nd, Bland KI and Heslin MJ: Recent insights into angiogenesis, apoptosis, invasion and metastasis in colorectal carcinoma. Ann Surg Oncol. 10:839–851. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Maeshima Y, Colorado PC, Torre A, Holthaus KA, Grunkemeyer JA, Ericksen MB, Hopfer H, Xiao Y, Stillman IE and Kalluri R: Distinct antitumor properties of a type IV collagen domain derived from basement membrane. J Biol Chem. 275:21340–21348. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Hamano Y and Kalluri R: Tumstatin, the NC1 domain of α3 chain of type IV collagen, is an endogenous inhibitor of pathological angiogenesis and suppresses tumor growth. Biochem Biophys Res Commun. 333:292–298. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Pasco S, Brassart B, Ramont L, Maquart FX and Monboisse JC: Control of melanoma cell invasion by type IV collagen. Cancer Detect Prev. 29:260–266. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Pasco S, Ramont L, Venteo L, Pluot M, Maquart FX and Monboisse JC: n vivo overexpression of tumstatin domains by tumor cells inhibits their invasive properties in a mouse melanoma model. Exp Cell Res. 301:251–265. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Kawaguchi T, Yamashita Y, Kanamori M, Endersby R, Bankiewicz KS, Baker SJ, Bergers G and Pieper RO: The PTEN/Akt pathway dictates the direct alphaVbeta3-dependent growth-inhibitory action of an active fragment of tumstatin in glioma cells n vitro and n vivo. Cancer Res. 66:11331–11340. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Chung IS, Son YI, Ko YJ, Baek CH, Cho JK and Jeong HS: Peritumor injections of purified tumstatin delay tumor growth and lymphatic metastasis in an orthotopic oral squamous cell carcinoma model. Oral Oncol. 44:1118–1126. 2008. View Article : Google Scholar : PubMed/NCBI

14 

Jeon HK, Chang KH, Kim KI and Chung IS: Functional expression of recombinant tumstatin in stably transformed Drosophila melanogaster S2 cells. Biotechnol Lett. 25:185–189. 2003. View Article : Google Scholar : PubMed/NCBI

15 

Alessandri G, Filippeschi S, Sinibaldi P, Mornet F, Passera P, Spreafico F, Cappa PM and Gullino PM: Influence of gangliosides on primary and metastatic neoplastic growth in human and murine cells. Cancer Res. 47:4243–4247. 1987.PubMed/NCBI

16 

Maeshima Y, Colorado PC and Kalluri R: Two RGD-independent alpha v beta 3 integrin binding sites on tumstatin regulate distinct anti-tumor properties. J Biol Chem. 275:23745–23750. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Maeshima Y, Sudhakar A, Lively JC, Ueki K, Kharbanda S, Kahn CR, Sonenberg N, Hynes RO and Kalluri R: Tumstatin, an endothelial cell-specific inhibitor of protein synthesis. Science. 295:140–143. 2002. View Article : Google Scholar : PubMed/NCBI

18 

Maeshima Y, Yerramalla UL, Dhanabal M, et al: Extracellular matrix-derived peptide binds to alpha(v)beta (3) integrin and inhibits angiogenesis. J Biol Chem. 276:31959–31968. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Monboisse JC, Garnotel R, Bellon G, Ohno N, Perreau C, Borel JP and Kefalides NA: The alpha 3 chain of type IV collagen prevents activation of human polymorphonuclear leukocytes. J Biol Chem. 269:25475–25482. 1994.PubMed/NCBI

20 

Li YJ, Sun LC, He Y, Liu XH, Liu M, Wang QM and Jin XM: The anti-tumor properties of two tumstatin peptide fragments in human gastric carcinoma. Acta Pharmacol Sin. 30:1307–1315. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Liu Y, Li J, Xu H, Zhang Y, Liu Y and Liu X: Mitochondria-mediated tumstatin peptide-induced HepG2 cell apoptosis. Int J Mol Med. 24:653–659. 2009. View Article : Google Scholar : PubMed/NCBI

22 

López-Hernández FJ, Ortiz MA and Piedrafita FJ: The extrinsic and intrinsic apoptotic pathways are differentially affected by temperature upstream of mitochondrial damage. Apoptosis. 11:1339–1347. 2006. View Article : Google Scholar : PubMed/NCBI

23 

Yin XM: Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 10:161–167. 2000. View Article : Google Scholar : PubMed/NCBI

24 

Maeshima Y, Manfredi M, Reimer C, Holthaus KA, Hopfer H, Chandamuri BR, Kharbanda S and Kalluri R: Identification of the anti-angiogenic site within vascular basement membrane-derived tumstatin. J Biol Chem. 276:15240–15248. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Oliveira-Ferrer L, Wellbrock J, Bartsch U, Penas EM, Hauschild J, Klokow M, Bokemeyer C, Fiedler W and Schuch G: Combination therapy targeting integrins reduces glioblastoma tumor growth through antiangiogenic and direct antitumor activity and leads to activation of the pro-proliferative prolactin pathway. Mol Cancer. 12:1442013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Hwang‑Bo J, Park JH and Chung IS: Tumstatin induces apoptosis mediated by Fas signaling pathway in oral squamous cell carcinoma SCC-VII cells. Oncol Lett 10: 1016-1022, 2015.
APA
Hwang‑Bo, J., Park, J., & Chung, I.S. (2015). Tumstatin induces apoptosis mediated by Fas signaling pathway in oral squamous cell carcinoma SCC-VII cells. Oncology Letters, 10, 1016-1022. https://doi.org/10.3892/ol.2015.3261
MLA
Hwang‑Bo, J., Park, J., Chung, I. S."Tumstatin induces apoptosis mediated by Fas signaling pathway in oral squamous cell carcinoma SCC-VII cells". Oncology Letters 10.2 (2015): 1016-1022.
Chicago
Hwang‑Bo, J., Park, J., Chung, I. S."Tumstatin induces apoptosis mediated by Fas signaling pathway in oral squamous cell carcinoma SCC-VII cells". Oncology Letters 10, no. 2 (2015): 1016-1022. https://doi.org/10.3892/ol.2015.3261
Copy and paste a formatted citation
x
Spandidos Publications style
Hwang‑Bo J, Park JH and Chung IS: Tumstatin induces apoptosis mediated by Fas signaling pathway in oral squamous cell carcinoma SCC-VII cells. Oncol Lett 10: 1016-1022, 2015.
APA
Hwang‑Bo, J., Park, J., & Chung, I.S. (2015). Tumstatin induces apoptosis mediated by Fas signaling pathway in oral squamous cell carcinoma SCC-VII cells. Oncology Letters, 10, 1016-1022. https://doi.org/10.3892/ol.2015.3261
MLA
Hwang‑Bo, J., Park, J., Chung, I. S."Tumstatin induces apoptosis mediated by Fas signaling pathway in oral squamous cell carcinoma SCC-VII cells". Oncology Letters 10.2 (2015): 1016-1022.
Chicago
Hwang‑Bo, J., Park, J., Chung, I. S."Tumstatin induces apoptosis mediated by Fas signaling pathway in oral squamous cell carcinoma SCC-VII cells". Oncology Letters 10, no. 2 (2015): 1016-1022. https://doi.org/10.3892/ol.2015.3261
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