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August-2015 Volume 10 Issue 2

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

RhoC is essential in TGF‑β1 induced epithelial‑mesenchymal transition in cervical cancer cells

  • Authors:
    • Xiaoqi He
    • Ying Qian
    • Huilan Cai
    • Shouhua Yang
    • Jing Cai
    • Zehua Wang

  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
  • Pages: 985-989
    |
    Published online on: May 27, 2015
       https://doi.org/10.3892/ol.2015.3287
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Abstract

Epithelial‑mesenchymal transition (EMT) is a critical process in the promotion of epithelial tumor progression and metastasis. The present study aimed to investigate the role of Ras homolog gene family, member C (RhoC) guanosine triphosphatase (GTPase) in transforming growth factor (TGF)‑β1 induced EMT. EMT occurred in human cervical carcinoma SiHa cells following TGF‑β1 stimulation for 4 days, as demonstrated by the appearance of mesenchymal morphology, reorganization of the actin cytoskeleton, reduced E‑cadherin expression and increased Vimentin expression, which was associated with increased RhoC expression and activity. However, EMT was not observed in cells that were pretreated with RhoC siRNA prior to TGF‑β1 stimulation. Downregulation of RhoC 4 days following TGF‑β1 stimulation was not able to reverse the existing EMT. In addition, TGF‑β1 promoted the invasion of the control SiHa cells but not that of the cells in which RhoC was downregulated. In conclusion, RhoC expression is activated by TGF‑β1, and sufficient RhoC expression levels are essential for TGF‑β1-induced EMT.
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Copy and paste a formatted citation
Spandidos Publications style
He X, Qian Y, Cai H, Yang S, Cai J and Wang Z: RhoC is essential in TGF‑β1 induced epithelial‑mesenchymal transition in cervical cancer cells. Oncol Lett 10: 985-989, 2015.
APA
He, X., Qian, Y., Cai, H., Yang, S., Cai, J., & Wang, Z. (2015). RhoC is essential in TGF‑β1 induced epithelial‑mesenchymal transition in cervical cancer cells. Oncology Letters, 10, 985-989. https://doi.org/10.3892/ol.2015.3287
MLA
He, X., Qian, Y., Cai, H., Yang, S., Cai, J., Wang, Z."RhoC is essential in TGF‑β1 induced epithelial‑mesenchymal transition in cervical cancer cells". Oncology Letters 10.2 (2015): 985-989.
Chicago
He, X., Qian, Y., Cai, H., Yang, S., Cai, J., Wang, Z."RhoC is essential in TGF‑β1 induced epithelial‑mesenchymal transition in cervical cancer cells". Oncology Letters 10, no. 2 (2015): 985-989. https://doi.org/10.3892/ol.2015.3287
Copy and paste a formatted citation
x
Spandidos Publications style
He X, Qian Y, Cai H, Yang S, Cai J and Wang Z: RhoC is essential in TGF‑β1 induced epithelial‑mesenchymal transition in cervical cancer cells. Oncol Lett 10: 985-989, 2015.
APA
He, X., Qian, Y., Cai, H., Yang, S., Cai, J., & Wang, Z. (2015). RhoC is essential in TGF‑β1 induced epithelial‑mesenchymal transition in cervical cancer cells. Oncology Letters, 10, 985-989. https://doi.org/10.3892/ol.2015.3287
MLA
He, X., Qian, Y., Cai, H., Yang, S., Cai, J., Wang, Z."RhoC is essential in TGF‑β1 induced epithelial‑mesenchymal transition in cervical cancer cells". Oncology Letters 10.2 (2015): 985-989.
Chicago
He, X., Qian, Y., Cai, H., Yang, S., Cai, J., Wang, Z."RhoC is essential in TGF‑β1 induced epithelial‑mesenchymal transition in cervical cancer cells". Oncology Letters 10, no. 2 (2015): 985-989. https://doi.org/10.3892/ol.2015.3287
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