Calligonum comosum extract inhibits diethylnitrosamine-induced hepatocarcinogenesis in rats

Abdo,Walied; Hirata,Akihiro; Shukry,Mostafa; Kamal,Tarek; Abdel-Sattar,Essam; Mahrous,Engi; Yanai,Tokuma

doi:10.3892/ol.2015.3313

Calligonum comosum extract inhibits diethylnitrosamine-induced hepatocarcinogenesis in rats

Authors:
- Walied Abdo
- Akihiro Hirata
- Mostafa Shukry
- Tarek Kamal
- Essam Abdel-Sattar
- Engi Mahrous
- Tokuma Yanai
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Affiliations: Department of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr el‑Sheikh 33516, Egypt, Division of Animal Experiment, Life Science Research Center, Gifu University, Gifu 501‑1193, Japan, Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr el‑Sheikh 33516, Egypt, Department of Biochemistry, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr el‑Sheikh 33516, Egypt, Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11562, Egypt, Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501‑1193, Japan
Published online on: June 3, 2015 https://doi.org/10.3892/ol.2015.3313
Pages: 716-722
Copyright: © Abdo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Calligonum comosum (C. comosum) is an Egyptian desert plant that contains polyphenol antioxidants. The present study examined the chemopreventive effect of an extract of C. comosum in a rat model of hepatocarcinogenesis. Male Wistar rats (n=40) were administered 100 mg/kg diethylnitrosamine (DEN) by intraperitoneal (i.p.) injection once a week for 3 weeks. Subsequently, depending on whether the rats received further administration of 0.8 mg/kg carbon tetrachloride (CCl4) i.p. once a week for 7 weeks and 100 mg/kg C. comosum extract in their diet for 7 weeks, the rats were divided into four groups as follows: Group 1, treatment with DEN alone; group 2, treatment with DEN and C. comosum extract; group 3, treatment with DEN and CCl4; and group 4, treatment with DEN, CCl4 and C. comosum extract. The supplementation of C. comosum extract significantly suppressed the elevation in serum liver enzyme levels, including aspartate aminotransferase, alanine transaminase and γ‑glutamyl transferase, and reduced the degree of oval cell proliferation induced by DEN and CCl4. In addition, C. comosum extract significantly decreased the number and area of glutathione S‑transferase placental form‑positive preneoplastic hepatic foci induced by DEN, with or without CCl4 treatment. To the best of our knowledge, the present study is the first to provide definitive evidence of the hepatoprotective and chemopreventive effects of C. comosum.

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