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Article

Effect of insulin on the mRNA expression of procollagen N-proteinases in chondrosarcoma OUMS-27 cells

  • Authors:
    • Sumeyya Akyol
    • İsmail Cömertoğlu
    • Ridvan Firat
    • Özlem Çakmak
    • Yunus Yukselten
    • Gönül Erden
    • Veli Ugurcu
    • Kadir Demircan
  • View Affiliations / Copyright

    Affiliations: Department of Medical Biology, Turgut Ozal University School of Medicine, Ankara, Ankara 06170, Turkey, Department of Medical Genetics, Faculty of Medicine, Mevlana University, Selcuklu, Konya 42003, Turkey, Division of Clinical Biochemistry Laboratory, Golbasi Hospital, Ministry of Health, Golbasi, Ankara 06830, Turkey, Department of Biology, Faculty of Education, Gazi University, Ankara, Ankara 06500, Turkey, Department of Medical Biology, Faculty of Medicine, Ankara University, Sihhiye, Ankara 06100, Turkey, Department of Clinical Biochemistry, Faculty of Medicine, Hacettepe University, Ankara, Ankara 06100, Turkey, Department of Medical Biochemistry, Faculty of Medicine, Dumlupinar University, Kutahya, Kutahya 43266, Turkey
  • Pages: 1091-1096
    |
    Published online on: June 4, 2015
       https://doi.org/10.3892/ol.2015.3317
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Abstract

Chondrosarcoma is one of the most common bone tumors, and at present, there is no non‑invasive treatment option for this cancer. The chondrosarcoma OUMS‑27 cell line produces proteoglycan and type II, IX, and XI collagens, which constitutes cartilage tissue. A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) proteases are a group of secreted proteases, which include the procollagen N‑proteinases ADAMTS‑2, ‑3 and ‑14. These procollagen N‑proteinases perform a role in the processing of procollagens to collagen and the maturation of type I collagen. The present study aimed to improve the understanding of the causes of metastasis, local invasion and resistance to chemo‑ and radiotherapy in chondrosarcoma, as well as the effect of insulin on cancer cells. The present study was designed to reveal the effects of insulin on procollagen N‑proteinases in chondrosarcoma OUMS‑27 cells. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) alone or in DMEM containing 10 µg/ml insulin. The medium was changed every other day for 11 days. The cells were harvested on days 1, 3, 7 and 11, and total RNA isolation was performed immediately following harvesting. The expression levels of ADAMTS2, ADAMTS3 and ADAMTS14 mRNA were estimated by reverse transcription‑quantitative polymerase chain reaction using appropriate primers. ADAMTS2 mRNA expression was found to be decreased on day 7 (P=0.028) and increased at day 11 compared with the control group (P=0.016). The increase in mRNA concentration at day 11 was significantly different compared to the concentrations on days 3 (P=0.047) and 7 (P=0.008). The expression of ADAMTS3 mRNA decreased immediately subsequent to insulin induction on day 1 compared with the control group (P=0.008). The most evident decrease in mRNA concentration was seen at day 7 subsequent to insulin induction (P=0.008). The present results demonstrated that ADAMTS2 and ADAMTS3 may perform a role in the invasion and metastasis of tumors, and may also possess proteolytic activity that results in the breakdown of the extracellular matrix (ECM). Insulin itself can modulate the biosynthesis of ECM macromolecules that are altered in diabetes through various pathways.
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1 

Qasem SA and DeYoung BR: Cartilage-forming tumors. Semin Diagn Pathol. 31:10–20. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Söderström M, Böhling T, Ekfors T, Nelimarkka L, Aro HT and Vuorio E: Molecular profiling of human chondrosarcomas for matrix production and cancer markers. Int J Cancer. 100:144–151. 2002. View Article : Google Scholar : PubMed/NCBI

3 

Yamamoto S, Tanaka K, Sakimura R, et al: Suberoylanilide hydroxamic acid (SAHA) induces apoptosis or autophagy-associated cell death in chondrosarcoma cell lines. Anticancer Res. 28:1585–1591. 2008.PubMed/NCBI

4 

Clark JC, Dass CR and Choong PF: Development of chondrosarcoma animal models for assessment of adjuvant therapy. ANZ J Surg. 79:327–336. 2009. View Article : Google Scholar : PubMed/NCBI

5 

Kunisada T, Miyazaki M, Mihara K, et al: A new human chondrosarcoma cell line (OUMS-27) that maintains chondrocytic differentiation. Int J Cancer. 77:854–859. 1998. View Article : Google Scholar : PubMed/NCBI

6 

Nishida K, Kunisada T, Shen ZN, Kadota Y, Hashizume K and Ozaki T: Chondrosarcoma and peroxisome proliferator-activated receptor. PPAR Res. 2008:2505682008. View Article : Google Scholar : PubMed/NCBI

7 

Akyol S, Yukselten Y, Cakmak O, et al: Hydrogen peroxide-induced oxidative damage in human chondrocytes: The prophylactic effects of Hypericum perforatum Linn extract on deoxyribonucleic acid damage, apoptosis and matrix remodeling by a disintegrin-like and metalloproteinase with thrombospondin motifs proteinases. Arch Rheumatol. 29:203–214. 2014. View Article : Google Scholar

8 

Akyol S, Acar M, Unal ZN, et al: The effects of caffeic acid phenethyl ester (CAPE), royal jelly, and curcumin on gene expression of ADAMTS −1, −5 and −9 in OUMS −27 chondrosarcoma cells: A preliminary study. Ann Paediatr Rheum. 2:27–37. 2013. View Article : Google Scholar

9 

Uysal S, Ünal ZN, Erdoğan S, et al: Augmentation of ADAMTS9 gene expression by IL-1β is reversed by NFκB and MAPK inhibitors, but not PI3 kinase inhibitors. Cell Biochem Funct. 31:539–544. 2013.PubMed/NCBI

10 

Przemyslaw L, Boguslaw HA, Elzbieta S and Malgorzata SM: ADAM and ADAMTS family proteins and their role in the colorectal cancer etiopathogenesis. BMB Rep. 46:139–150. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Demircan K, Cömertoğlu I, Akyol S, et al: A new biological marker candidate in female reproductive system diseases: Matrix metalloproteinase with thrombospondin motifs (ADAMTS). J Turk Ger Gynecol Assoc. 15:250–255. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Demircan K, Hirohata S, Nishida K, et al: ADAMTS-9 is synergistically induced by interleukin-1beta and tumor necrosis factor alpha in OUMS-27 chondrosarcoma cells and in human chondrocytes. Arthritis Rheum. 52:1451–1460. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Guo F, Lai Y, Tian Q, Lin EA, Kong L and Liu C: Granulin-epithelin precursor binds directly to ADAMTS-7 and ADAMTS-12 and inhibits their degradation of cartilage oligomeric matrix protein. Arthritis Rheum. 62:2023–2036. 2010.PubMed/NCBI

14 

Porter S, Clark IM, Kevorkian L and Edwards DR: The ADAMTS metalloproteinases. Biochem J. 386:15–27. 2005. View Article : Google Scholar : PubMed/NCBI

15 

Colige A, Nuytinck L, Hausser I, et al: Novel types of mutation responsible for the dermatosparactic type of Ehlers-Danlos syndrome (Type VIIC) and common polymorphisms in the ADAMTS2 gene. J Invest Dermatol. 123:656–663. 2004. View Article : Google Scholar : PubMed/NCBI

16 

Le Goff C, Somerville RP, Kesteloot F, et al: Regulation of procollagen amino-propeptide processing during mouse embryogenesis by specialization of homologous ADAMTS proteases: insights on collagen biosynthesis and dermatosparaxis. Development. 133:1587–1596. 2006. View Article : Google Scholar : PubMed/NCBI

17 

Fernandes RJ, Hirohata S, Engle JM, et al: Procollagen II amino propeptide processing by ADAMTS-3. Insights on dermatosparaxis. J Biol Chem. 276:31502–31509. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Poonpet T, Honsawek S, Tammachote N, Kanitnate S and Tammachote R: ADAMTS14 gene polymorphism associated with knee osteoarthritis in Thai women. Genetics Mol Res. 12:5301–5309. 2013. View Article : Google Scholar

19 

Otsu K, Geary ES and Stevens RL: Aberrant regulation of the metabolism of the insulin receptor in Swarm rat chondrosarcoma chondrocytes. Biochem J. 254:203–209. 1988.PubMed/NCBI

20 

Stevens RL and Hascall VC: Characterization of proteoglycans synthesized by rat chondrosarcoma chondrocytes treated with multiplication-stimulating activity and insulin. J Biol Chem. 256:2053–2058. 1981.PubMed/NCBI

21 

Foley TP Jr, Nissley SP, Stevens RL, et al: Demonstration of receptors for insulin and insulin-like growth factors on Swarm rat chondrosarcoma chondrocytes. Evidence that insulin stimulates proteoglycan synthesis through the insulin receptor. J Biol Chem. 257:663–669. 1982.PubMed/NCBI

22 

Stevens RL, Schwartz LB, Austen KF, Lohmander LS and Kimura JH: Effect of tunicamycin on insulin binding and on proteoglycan synthesis and distribution in Swarm rat chondrosarcoma cell cultures. J Biol Chem. 257:5745–5750. 1982.PubMed/NCBI

23 

Uğurcu V, Akyol S, Altuntaş A, Firat R, et al: The effects of insulin on the expression levels of ADAMTS6 & 19 in OUMS-27 cells. Dicle Med J. 41:451–456. 2014. View Article : Google Scholar

24 

Firat R, Akyol S, Kurşunlu SF, et al: ADAMTS13 expression in human chondrosarcoma cells induced by insulin. J Clin Exp Invest. 5:226–232. 2014. View Article : Google Scholar

25 

Le Goff C and Cormier-Daire V: The ADAMTS(L) family and human genetic disorders. Hum Mol Genet. 20:R163–R167. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Hartigan N, Garrigue-Antar L and Kadler KE: Bone morphogenetic protein-1 (BMP-1). Identification of the minimal domain structure for procollagen C-proteinase activity. J Biol Chem. 278:18045–18049. 2003. View Article : Google Scholar : PubMed/NCBI

27 

Nusgens BV, Goebels Y, Shinkai H and Lapière CM: Procollagen type III N-terminal endopeptidase in fibroblast culture. Biochem J. 191:699–706. 1980.PubMed/NCBI

28 

Siefert SA and Sarkar R: Matrix metalloproteinases in vascular physiology and disease. Vascular. 20:210–216. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Peng WJ, Yan JW, Wan YN, et al: Matrix metalloproteinases: A review of their structure and role in systemic sclerosis. J Clin Immunol. 32:1409–1414. 2012. View Article : Google Scholar : PubMed/NCBI

30 

Deryugina EI and Quigley JP: Matrix metalloproteinases and tumor metastasis. Cancer Metastasis Rev. 25:9–34. 2006. View Article : Google Scholar : PubMed/NCBI

31 

Tang BL: ADAMTS: A novel family of extracellular matrix proteases. Int J Biochem Cell Biol. 33:33–44. 2001. View Article : Google Scholar : PubMed/NCBI

32 

Zhou H, Hickford JG and Fang Q: A premature stop codon in the ADAMTS2 gene is likely to be responsible for dermatosparaxis in Dorper sheep. Anim Genet. 43:471–473. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Colige A, Vandenberghe I, Thiry M, et al: Cloning and characterization of ADAMTS-14, a novel ADAMTS displaying high homology with ADAMTS-2 and ADAMTS-3. J Biol Chem. 277:5756–5766. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Matullo G, Guarrera S, Betti M, et al: Genetic variants associated with increased risk of malignant pleural mesothelioma: a genome-wide association study. PLoS One. 8:e612532013. View Article : Google Scholar : PubMed/NCBI

35 

Carinci F, Lo Muzio L, Piattelli A, et al: Potential markers of tongue tumor progression selected by cDNA microarray. Int J Immunopathol Pharmacol. 18:513–524. 2005.PubMed/NCBI

36 

Lee CW, Hwang I, Park CS, et al: Expression of ADAMTS −2, −3, −13 and −14 in culprit coronary lesions in patients with acute myocardial infarction or stable angina. J Thromb Thrombolysis. 33:362–370. 2012. View Article : Google Scholar : PubMed/NCBI

37 

Porter S, Scott SD, Sassoon EM, et al: Dysregulated expression of adamalysin-thrombospondin genes in human breast carcinoma. Clin Cancer Res. 10:2429–2440. 2004. View Article : Google Scholar : PubMed/NCBI

38 

Bolz H, Ramírez A, von Brederlow B and Kubisch C: Characterization of ADAMTS14, a novel member of the ADAMTS metalloproteinase family. Biochim Biophys Acta. 1522:221–225. 2001. View Article : Google Scholar : PubMed/NCBI

39 

Goertsches R, Comabella M, Navarro A, Perkal H and Montalban X: Genetic association between polymorphisms in the ADAMTS14 gene and multiple sclerosis. J Neuroimmunol. 164:140–147. 2005. View Article : Google Scholar : PubMed/NCBI

40 

Rodriguez-Lopez J, Pombo-Suarez M, Loughlin J, et al: Association of a nsSNP in ADAMTS14 to some osteoarthritis phenotypes. Osteoarthritis Cartilage. 17:321–327. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Al Nakouzi N, Bawa O, Le Pape A, et al: The IGR-CaP1 xenograft model recapitulates mixed osteolytic/blastic bone lesions observed in metastatic prostate cancer. Neoplasia. 14:376–387. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Akyol S, Cömertoğlu İ, Firat R, Çakmak Ö, Yukselten Y, Erden G, Ugurcu V and Demircan K: Effect of insulin on the mRNA expression of procollagen N-proteinases in chondrosarcoma OUMS-27 cells. Oncol Lett 10: 1091-1096, 2015.
APA
Akyol, S., Cömertoğlu, İ., Firat, R., Çakmak, Ö., Yukselten, Y., Erden, G. ... Demircan, K. (2015). Effect of insulin on the mRNA expression of procollagen N-proteinases in chondrosarcoma OUMS-27 cells. Oncology Letters, 10, 1091-1096. https://doi.org/10.3892/ol.2015.3317
MLA
Akyol, S., Cömertoğlu, İ., Firat, R., Çakmak, Ö., Yukselten, Y., Erden, G., Ugurcu, V., Demircan, K."Effect of insulin on the mRNA expression of procollagen N-proteinases in chondrosarcoma OUMS-27 cells". Oncology Letters 10.2 (2015): 1091-1096.
Chicago
Akyol, S., Cömertoğlu, İ., Firat, R., Çakmak, Ö., Yukselten, Y., Erden, G., Ugurcu, V., Demircan, K."Effect of insulin on the mRNA expression of procollagen N-proteinases in chondrosarcoma OUMS-27 cells". Oncology Letters 10, no. 2 (2015): 1091-1096. https://doi.org/10.3892/ol.2015.3317
Copy and paste a formatted citation
x
Spandidos Publications style
Akyol S, Cömertoğlu İ, Firat R, Çakmak Ö, Yukselten Y, Erden G, Ugurcu V and Demircan K: Effect of insulin on the mRNA expression of procollagen N-proteinases in chondrosarcoma OUMS-27 cells. Oncol Lett 10: 1091-1096, 2015.
APA
Akyol, S., Cömertoğlu, İ., Firat, R., Çakmak, Ö., Yukselten, Y., Erden, G. ... Demircan, K. (2015). Effect of insulin on the mRNA expression of procollagen N-proteinases in chondrosarcoma OUMS-27 cells. Oncology Letters, 10, 1091-1096. https://doi.org/10.3892/ol.2015.3317
MLA
Akyol, S., Cömertoğlu, İ., Firat, R., Çakmak, Ö., Yukselten, Y., Erden, G., Ugurcu, V., Demircan, K."Effect of insulin on the mRNA expression of procollagen N-proteinases in chondrosarcoma OUMS-27 cells". Oncology Letters 10.2 (2015): 1091-1096.
Chicago
Akyol, S., Cömertoğlu, İ., Firat, R., Çakmak, Ö., Yukselten, Y., Erden, G., Ugurcu, V., Demircan, K."Effect of insulin on the mRNA expression of procollagen N-proteinases in chondrosarcoma OUMS-27 cells". Oncology Letters 10, no. 2 (2015): 1091-1096. https://doi.org/10.3892/ol.2015.3317
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