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Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma

  • Authors:
    • Zhenshun Cheng
    • Qiuyue Tan
    • Weijun Tan
    • Li Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430070, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 641-646
    |
    Published online on: June 5, 2015
       https://doi.org/10.3892/ol.2015.3329
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Abstract

The aim of the present study was to determine the effect of cigarette smoke on the expression of Notch proteins in lung adenocarcinoma (LAC). Protein expression levels of Notch1 and Notch3 were analyzed using immunohistochemistry in 102 human LAC specimens. Of these, 52 were obtained from smokers and 50 from non‑smokers. In addition, cigarette smoke extract (CSE) at varying concentrations (1, 2.5 and 5%) was administered to A549 cells. The expression of Notch1 and Notch3 protein was then detected by western blot analysis at different time points (0, 8, 24 and 48 h). Of the 102 LAC specimens, 42 (41.2%) were positive for Notch1 and 63 (61.8%) were positive for Notch3. There was no significant difference in the level of Notch1 expression between smokers and non‑smokers with LAC (P>0.05). The positive rate and staining intensity of Notch3 expression were increased in the smokers compared with the non‑smokers (P<0.05). The expression of Notch3 protein in A549 cells increased in a time‑ and dose‑dependent manner following treatment with CSE, whilst the expression of Notch1 protein appeared stable. The results suggested that cigarette smoke was able to induce the expression of Notch3, not Notch1, protein in LAC. The data revealed an upregulation of Notch3 in LAC following cigarette smoke exposure. Such findings may provide a novel therapeutic target for the treatment of LAC.
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Copy and paste a formatted citation
Spandidos Publications style
Cheng Z, Tan Q, Tan W and Zhang L: Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma. Oncol Lett 10: 641-646, 2015.
APA
Cheng, Z., Tan, Q., Tan, W., & Zhang, L. (2015). Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma. Oncology Letters, 10, 641-646. https://doi.org/10.3892/ol.2015.3329
MLA
Cheng, Z., Tan, Q., Tan, W., Zhang, L."Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma". Oncology Letters 10.2 (2015): 641-646.
Chicago
Cheng, Z., Tan, Q., Tan, W., Zhang, L."Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma". Oncology Letters 10, no. 2 (2015): 641-646. https://doi.org/10.3892/ol.2015.3329
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng Z, Tan Q, Tan W and Zhang L: Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma. Oncol Lett 10: 641-646, 2015.
APA
Cheng, Z., Tan, Q., Tan, W., & Zhang, L. (2015). Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma. Oncology Letters, 10, 641-646. https://doi.org/10.3892/ol.2015.3329
MLA
Cheng, Z., Tan, Q., Tan, W., Zhang, L."Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma". Oncology Letters 10.2 (2015): 641-646.
Chicago
Cheng, Z., Tan, Q., Tan, W., Zhang, L."Cigarette smoke induces the expression of Notch3, not Notch1, protein in lung adenocarcinoma". Oncology Letters 10, no. 2 (2015): 641-646. https://doi.org/10.3892/ol.2015.3329
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