Expression of human protection of telomere 1 correlates with telomere length and radiosensitivity in the human laryngeal cancer Hep-2 cell line

  • Authors:
    • Han Lei
    • Dali Feng
    • Fuxiang Zhou
    • Hui Xu
    • Tian Tang
    • Haijun Yu
    • Conghua Xie
    • Yunfeng Zhou
  • View Affiliations

  • Published online on: June 5, 2015     https://doi.org/10.3892/ol.2015.3332
  • Pages: 1149-1154
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The close association between telomere length and radiosensitivity has been established by several studies. There is also a hypothesis that telomere length may be regulated by human protection of telomere 1 (hPOT1) in human carcinoma cells. In the present study, the hPOT1 level between the radioresistant Hep‑2R cells and the wild‑type were compared, and the results showed that the hPOT1 gene was upregulated in the radioresistant Hep‑2R cell lines compared with the wild‑type. This suggested that the expression level of hPOT1 correlates with radiosensitivity. Additionally, an hPOT1‑directed short hairpin (sh)RNA plasmid was constructed and transferred into the Hep‑2R cells, which lead to telomere shortening, an increase in apoptosis and markedly decreased growth of the RNAi‑Hep‑2R cell line. These results demonstrate that hPOT1‑directed shRNAs are associated with telomere length and radiosensitivity, and maybe a potent sensitizer for laryngeal cancer radiotherapy.
View Figures
View References

Related Articles

Journal Cover

August-2015
Volume 10 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Lei H, Feng D, Zhou F, Xu H, Tang T, Yu H, Xie C and Zhou Y: Expression of human protection of telomere 1 correlates with telomere length and radiosensitivity in the human laryngeal cancer Hep-2 cell line. Oncol Lett 10: 1149-1154, 2015
APA
Lei, H., Feng, D., Zhou, F., Xu, H., Tang, T., Yu, H. ... Zhou, Y. (2015). Expression of human protection of telomere 1 correlates with telomere length and radiosensitivity in the human laryngeal cancer Hep-2 cell line. Oncology Letters, 10, 1149-1154. https://doi.org/10.3892/ol.2015.3332
MLA
Lei, H., Feng, D., Zhou, F., Xu, H., Tang, T., Yu, H., Xie, C., Zhou, Y."Expression of human protection of telomere 1 correlates with telomere length and radiosensitivity in the human laryngeal cancer Hep-2 cell line". Oncology Letters 10.2 (2015): 1149-1154.
Chicago
Lei, H., Feng, D., Zhou, F., Xu, H., Tang, T., Yu, H., Xie, C., Zhou, Y."Expression of human protection of telomere 1 correlates with telomere length and radiosensitivity in the human laryngeal cancer Hep-2 cell line". Oncology Letters 10, no. 2 (2015): 1149-1154. https://doi.org/10.3892/ol.2015.3332